Busy. Please wait.
or

show password
Forgot Password?

Don't have an account?  Sign up 
or

Username is available taken
show password

why


Make sure to remember your password. If you forget it there is no way for StudyStack to send you a reset link. You would need to create a new account.
We do not share your email address with others. It is only used to allow you to reset your password. For details read our Privacy Policy and Terms of Service.


Already a StudyStack user? Log In

Reset Password
Enter the associated with your account, and we'll email you a link to reset your password.
Don't know
Know
remaining cards
Save
0:01
To flip the current card, click it or press the Spacebar key.  To move the current card to one of the three colored boxes, click on the box.  You may also press the UP ARROW key to move the card to the "Know" box, the DOWN ARROW key to move the card to the "Don't know" box, or the RIGHT ARROW key to move the card to the Remaining box.  You may also click on the card displayed in any of the three boxes to bring that card back to the center.

Pass complete!

"Know" box contains:
Time elapsed:
Retries:
restart all cards
share
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.

  Normal Size     Small Size show me how

UND 362 embedding

QuestionAnswer
what is the purpose of embedding (blocking/casting) encase tissue uniformly, create uniform density, demonstrate a desired structure
what types of media are used in embedding paraffin, celloidin/nitro cellulose, agar/gelatins, resins
give a positive and negative to paraffin per tissue inexpensive/universally used, NEG. heat used in processing step (infiltration) damages IHC
what is celloiding/nitro cellulose used for? brain microtomy (requires no heat, but takes a LONG time)
what is a drawback to using agar/gelatin takes a LONG time to process
give 4 factors and a disadv for using resins used for EM, some use heat or UV to polymerize, need glass or diamond knife, need special microtome attachment if used for light microscope. disadv - many resins are toxic/carcinogenic
what occurs if paraffin is rapidly cooled when embedding smaller crystal size therefore it will adhere closer to margin of tissue
give factors about metal molds various sizes, need to be cleaned, can be reused
give factors about plastic molds no cleanup, have static electricity, not reusable, biopsies can get stuck to edges
give the gen. rules and QC to embedding block label on top, tissue face down, light pressure applied to embed flat. QC # of specs in cassette, specific orientation, verification to accompanying logs
give steps for embedding *one cassette at a time*. Select mold size, fill mold w/paraffin, orient spec. embed on same plane, cover with cass. and fill w/ wax, cool immediately
describe large flat tissue orientation use pressure so all is at same level, flat edge down
describe elongated tissue orientation diagonal, narrow surface to microtome knife first
describe multiple desity tissue (bone and soft tissue) orientation soft tissue must be present to knife first
describe tubular tissue orientation (fall, vas def, app, temp. artery) - done in cross section (tire on its side)
describe epithelial (w/ wall) tissue orientation (ie GI, skin, cystic structure) All are done on edge (cystic structure ala dome would have cut/flat side down)
describe multiple spec tissue orientation done in a line (if skin it would have that cut last by knife)
describe bone tissue orientation put in diagonally ( with hard surface cut last)
what is forcep metastasis fragments accidentally moved from one block to another
describe needle bx's tissue orientation either verticle or diagonal (not desirable is random nor parallel horizontally)
if tissue is inked or has a notch on the top how should it be orientated ink side or notch up (per the book)
Created by: mustangvxd