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SIB2004 1
An Introduction To Protein Structure
Question | Answer |
---|---|
The protein Ribonuclease contains 8 cysteine amino acids. The protein is denatured in 8M urea and a reducing agent is added. Assume that all cysteine bonds are broken and can recombine randomly.How many combinations of disulphide bonds are possible? | Refer to notes |
Which of the following amino acids would you NOT expect to find in the hydrophobic core of a globular protein? a) Alanine b) Phenylalanine c) Valine d) Serine | Refer to notes |
Which amino acid side chain would have a no charged groups at pH 7.0? a) Arginine b) Glutamate c) Lysine d) Alanine | Refer to notes |
The spatial arrangement of helices and strands within a single polypeptide chain is called the: a) Primary structure b) Secondary structure c) Tertiary structure d) Quaternary structure | Refer to notes |
Which of the following amino acids is polar? a) Alanine b) Valine c) Lysine d) Leucine | Refer to notes |
What are the four levels of structure determine the shape of proteins? | Primary, Seconday, Tertiary and Quaternary. |
What is a Primary Structure? | The linear sequence of amino acids. |
What is a Secondary Structure? | the localized organization of parts of a polypeptide chain (e.g., the alpha helix or beta-pleated sheet). |
What is a Tertiary Structure? | The overall, three-dimensional arrangement of the polypeptide chain. |
What is a Quaternary Structure? | The association of two or more polypeptides into a multi-subunit complex. |
What is a protein? | Proteins are single, unbranched chains of amino acid monomers. |
How many amino acids are there? | 20. |
What determines a protein's 3D structure? (conformation) | A protein's amino acid sequence. |
What determines the function of a protein? | A protein’s structure. |
What is the structural form of L-Alanine? | Refer to notes. |
What is the structural form of D-Alanine? | Refer to notes. |
All amino acids have the same general structure but what is different for each of them? | The side chain (R group) of each is different. |
What are the nonpolar, aliphatic R groups? | Glycine, Alanine, Leucine, Methionine, Isoleucine and Valine. |
What are the aromatic R groups? | Phenylalanine, Tyrosine and Tryptophan. |
What are the polar, uncharged R groups? | Serine, Threonine, Cysteine, Proline, Asparagine and Glutamine. |
What are the positively charged R groups? | Lysine, Arginine and Histidine. |
What are the negatively charged R groups? | Asparate and Glutamate. |
In what form do isolated amino acids exist in solution? | Zwitterionic form. |
In what form do proteins exist in solution? | A polypeptide chain, with a positive N-terminus, negative C-terminus. |
What type of bond is usually represented by a dashed line? | Hydrogen bonds. |