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Shared characteristics G- bacilli can culture on blood or MacConkey agar facultative anaerobes saccharolytic oxidase negative (-)
Genera included Citrobacter Enterobacter Escherichia Klebsiella Proteus Salmonella Shigella Yersinia
Citrobacter and Enterobacter motile human/animal EXTRAINTESTINAL pathogen opportunistic mixed cultures
Escherichia coli serovars based on protein-composed fimbral (F) antigens CFA-I through CFA-IV cause human disease F-4, -5, -6, and -41 cause porcine disease
Enterohemorrhagic E. coli motile lower small intestine bloody diarrhea Shigalike toxinf I and II most common serovar = O157:H7
Enteroinvasive E. coli similar to Shigella dysentery large intestines and colon
Enteropathogenic E. coli entercolitis similar to Salmonella enterotoxins and shigalike toxins
Human Enterotoxigenic E. coli transmission: ingestion or overproliferation of normal flora watery diarrhea and dehydration --> death stomach and small intestine distended with gas and fluid most produce complete hemolysis
Human Enterotoxigenic E. coli virulence factors FIMBRAE: coded for on plasmids, allow colonization HEAT-LAIBLE: destroyed @ 56C for 30m. Holotoxin. MW 85,000. 1 A increases cAMP inhibits Na+ channel. The 5B subunits bind HEAT-STABLE A&B: nonantigenic, MW=2,000. A increases cGMP. B is anti-absorptive
Enterotoxigenic E. coli w/ heat-laible enterotoxins DX LIGATED ILEAL LOOP OF LIVE RABBITS: inject w/ supernatant, see fluid accumulation. *can also use for heat-stable* TISSUE CULTURE METHOD W/ MOUSE ADRENAL OR HAMSTER OVARY: expose tissue to supernatant, look for morpholigical response to incr. of cAMP
Human Enterotoxigenic E. coli w/ heat-laible enterotoxins DX INFANT MOUSE TEST inject supernatant into infant mouse stomach, wait 4 hr, look for fluid accumulation :(
Klebsiella nonmotile opportunistic EXTRAINTESTINAL infections (cystitis, mastitis, metritis) pure culture potent endotoxin --> endotoxic shock
Proteus motile opportunistic EXTRAINTESTINAL infections mixed cultures cause UTI's in humans/domestic animals
Salmonella (basic info) all but avian-adapted are motile w/ PERITRICHOUS flagella nonhemolytic on blood agar colorless on MacConkey
Salmonella Serovars based on O antigens (#), H phase 1 antigens (a, b, c...) and H pahse 2 antigens (#) S. Dublin and S. Typhi also have VI carbohydrate capsular antigens NOT used for serotyping
Salmonella Cholerasuis pigs acute septicemia with endotoxemia, fever high MR or chronic pneumonia enteritis is rare
Salmonella Dublin cow acute enteritis with bloody diarrhea esp. feedlot cattle that have been shipped
Salmonella Typhi HUMAN TYPHOID transmission: ingestion septicemia, fever, GI symptoms VI antigen important unlike other salmonella serovars, ANAREOGENIC in glucose broth
Salmonalla Typhimurium in cows: similar to S. Dublin in horses: entercolitis, can localize to other tissues, high MR, antibiotic-induced in pigs: causes Porcine Salmonellae Entercolitis
S. Typhimurium and Porcine Entercolitis young pigs, four stages: 1)ingestion 2)infection/invasion of GI tract 3)proliferation in intestinal wall 4)bacteremia/septicemia ileum, cecum, coloncytotoxins, enterotoxins, LPS Serogroup B, C, D, or E; use agglutination tests with sero-specific antiser
Shigella dysenteriae nonmotile no hemolysis colorledd MacConkey colonies causes Human Bacilliary Dysentery
Human Bacilliary Dyesntery ingestion watery feces w/ blood + mucus polysaccharide capsule, LPS, cytotoxins (holotoxins, MW 62,000, inhibit protein synth in euk cells, also called "verotoxins", include shiga tox I + II), enterotoxins cecum + lg. intestine
Yersinia entercolitica causes enteritis in humans (milk/meat ingestion) swine reservoir ileitis, mesenteric lymphadenitis, septicemia diarrhea temp-dependent motility, peritrichous falgella
Yersinia pseudotuberculosis psuedoTB in some animals pseudoTB in humans: lesions w/ caseous necrosis temp-dependent motility w/ peritrichous flagella
Yersinia pestis nonmotile extraintestinal pathogen of rodents causes human plague capsule
Created by: jesters



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