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Fixative Formulas

Formulas for mixing fixatives - CPTC

Phosphate-Buffered Glutaraldehyde Sodium Phosphate, Monobasic - 1.9 g, Sodium Hydroxide - 0.43 g, Distilled Water - 100 ml, *Mix well then add Glutaraldehyde 25% solution - 18 ml
B-5 Working Solution B-5 Stock - 20 ml, Formaldehyde - 2 ml
Bouin Picric Acid (Saturated Aqueous Solution 1.2%)- 750 ml, Formaldehyde 37-40% - 250 ml, Acetic Acid (glacial) - 50 ml
Modified Millonig Formalin Formaldehyde 37-40% - 100 ml, Distilled Water 900 ml, Sodium Phosphate, monobasic - 18.6 g, Sodium Hydroxide 4.2 g
Orth Solution Potassium dichromate - 2.5 g, Sodium Sulfate - 1 g, Distilled Water - 100 ml, *Just before use add Formaldehyde 37-40% - 10 ml
Calcium Formalin Formaldehyde 37-40% - 100 ml, Calcium Chloride - 10 g, Distilled Water - 900 ml
10% Neutralized Formalin Formaldehyde 37-40% - 100 ml, Distilled Water - 900 ml, Calcium/Magnesium Carbonate to excess
10% Neutral Buffered Formalin Formaldehyde 37-40% - 100 ml, Distilled Water - 900 ml, Sodium Phosphate, monobasic - 4 g, Sodium Phosphate, dibasic - 6.5 g
10% Aqueous Formalin Formaldehyde 37-40% - 100 ml, Distilled Water - 900 ml
Michel Transport Medium Anhydrous Citric Acid - 4.803 g. Ammonium Sulfate - 412.3 g, N-Ethylmaleimide - 625 mg(0.625g), Magnesium Sulfate - 1.23g, Distilled Water - to 1 liter
10 % Formalin Saline Formaldehyde 37-40% - 100 ml, Sodium Chorlide - 9 g, Distilled Water - 900 ml
Formalin Ammonium Bromide Formaldehyde 37-40% - 150 ml, Ammonium Bromide - 20g, Distilled Water - 850 ml
Acetate Formalin Formaldehyde 37-40% - 100ml, Sodium Acetate - 20g, Distilled Water - 900ml
Unbuffered Aqueous Zinc Formalin Zinc Sulfate - 20g, Distilled Water - 900ml, *stir until dissolved and add, Formaldehyde 37-40% - 100ml
Aqueous Zinc Formalin Zinc Sulfate(heptahydrate) - 10g, Formaldehyde 37-40% - 100ml, distilled water 900ml
Hollande Solution Copper Acetate - 25g, Picric Acid - 40g, Formaldehyde 37-40% - 100ml, Acetic Acid 15ml, Distilled Water - 1,000ml
Alcoholic Zinc Chloride Formalin Zinc Chloride - 4.5g, Distilled/Deionized Water - 1,000ml, *Stir until dissolved and add: Isopropyl Alcohol(99%) - 2,000ml, Formaldehyde 37-40% - 400ml
Zamboni (PAF) Paraformaldehyde - 20g, Picric Acid, saturated aqueous(double filtered) - 150ml, *Heat to 60 degrees, add Aqueous Sodium Hydroxide - 2.52%(dropwise to alkalinize the solution) Buffer: Sodium Phosphate Mono - 3.31g, SodiumPhosphate Di -17.88g, DW - 1,000ml
Gendre Solution Alcoholic, 95% saturated with Picric Acid - 800ml, Formaldehyde 37-40% - 150ml, Glacial Acetic Acid - 50ml
Zenker Working Solution Zenker-Helly Stock - 95ml, Acetic Acid, Glacial - 5 ml
Helly Working Solution Zenker-Helly Stock - 95ml, Formaldehyde 37-40% - 5ml
B-5 Stock Mercuric Chloride - 12g, Sodium Acetate(anhydrous) - 2.5g, Distilled Water - 200ml
Clarke Fluid Absolute Alcohol - 300ml, Acetic Acid(glacial) - 100ml
PBS - 10% Sucrose Solution Stock PBS Solution - 4ml, Distilled Water - 96ml, Sucrose - 10g
Carnoy Solution Absolute Ethyl Alcohol - 60ml, Chloroform - 30ml, Acetic Acid,glacial - 10ml
PBS Buffer Stock Solution Potassium Phosphate Dibasic - 188g, Sodium Phosphate Monobasic - 33g, Sodium Chloride - 180g, Distilled Water - 800ml
Zenker & Helly Stock Solution Mercuric Chloride - 50g, Potassium Dichromate - 25g, Sodium Sulfate(optional) - 10g, Distilled Water - 1,000ml
Created by: Megerimc



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