Micro ch10 Biotech Test
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| A. DNA to be sequenced cut into large number of short fragments and separated; test strands denatured to expose single strands to act as templates for complementary strands; fragments divided into 4 separate tubes w/ fluorescent/radioactive primers indicatinB. basic scienceC. technique that separates fragments of DNA using electrophoresis and identifies them by hybridizationD. synthetic oligonucleotides; landmarks for where DNA amplification should beginE. restriction fragments of differing lengths; allow direct comparison of the DNA of two different organisms at a specific siteF. Human growth hormone, Insulin, Clotting factor VIIIG. enzyme; converts RNA into DNAH. A recombinant organism is produced through the introduction of foreign genes that have been inserted into their genome purposefully. They could also be called transgenic or genetically modified.I. sequences of DNA that are identical when read from the 5’ to 3’ direction of one strand and the 5’ to 3’ direction on the other strand. (ex. eye, racecar)J. named by combining first letter of bacterial genus, first two letters of the species, and endonuclease number. EcoRI is the first endonuclease in E. coli and HindIII is the third nuclease in Haemophilus influenzaeK. pieces of DNA produced by restriction endonucleasesL. can be made from mRNA, tRNA, rRNA, et al. Provides valuable means of synthesizing eukaryotic genes from mRNA transcripts.M. A clone is an animal that is genetically the same as its parent. It shows no recombination and no variation.N. DNAs formed in first cycle become amplicons upon denaturization; heating target DNA to 94 degrees C to separate it into two strands; then system is cooled to between 50 and 65 depending on nucleotide sequenceO. Recombinant human insulin; it was previously obtained from pigs.P. processing must be carried out at a high temperature to keep strands separated, so polymerases are isolated from thermophilic bacteriaQ. clips polynucleotide strands crosswise at selected positions; can recognize/clip palindromes, recognize foreign DNA; can break phosphodiester bonds; originate in bacterial cells; used in labs to cleave the strand at desired sitesR. uniting of two different nucleic acids at their complementary regions; different combos possible (single DNA with other single RNA or DNA, RNA with other RNA; allows for specially formulated oligonucleotide tracers (gene probes)S. oligonucleotide tracers; short stretch of DNA of a known sequence that will base-pair w/ stretch of DNA w/ complementary sequence; detects specific sequences in unknown samples; Reactions revealed by placing photographic film in contact w/ test reaction.T. Protection from foreign DNA (such as that from phages). |
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