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Cytology Test

Enter the letter for the matching Answer
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1.
Preferred cytology fixative:
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2.
Cell block
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3.
Pap stain purpose
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4.
Direct scrapings for viral lesions (Tzank smears) treatment:
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5.
Problem: bloody specimens:
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6.
SurePath System
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7.
Alcohol vs. formalin fixation
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8.
OG-6 reagents
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Bodys fluids pleural, peritoneal, pericardial, ascites treatment:
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10.
Cell block for specimen w/ loose cellular material - agar method:
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11.
Cell block for specimen w/ cellular material entrapped in mucus:
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Washings - bronchial, esophageal, & gastric treatment:
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CSF treatment:
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14.
Nickel smear method
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15.
Direct smears include:
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Diagnostic cytology
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17.
Cytopreparation for sample with poor cellularity?
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18.
Sparsely cellular specimens
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19.
Cytopreparation
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20.
Cell block for specimen w/ loose cellular material - albumin method:
A.
To remaining sediment add melted 4% agar, mix gently, centrifuge 10 min, 2,000 rpm, refrigerate until agar solid, remove from tube, cut away cellular material, bisect, place in cassette w/ common cut side down, formalin process routinely
B.
Smear material from scraper/brush in circular motion onto slide in area the size of a nickel, fix immediately w/ 95% ethanol or spray fixative, avoid cotton swabs, avoid feathered-edge smears
C.
Alcohol better b/c crisp fixation of nuclear chromatin, avoid formalin - causes different nuclear chromatin patterns which may impact diagnosis
D.
Cytocentrifuge
E.
Sample w/ not many cells in suspension can be made more conc'd by Cytospin or centrifugation preparation: 1mL properly prepared cell suspension in funnel of cytospin, centrifuge, cells will be deposited on slide & fluid absorbed by filter paper
F.
To remaining sediment add 30% BSA, mix w/ stick, add 95% alcohol, place mass in cassette, may wrap w/ lens paper, if too large submit portion, formalin process routinely
G.
95% ethanol, rapid fixation important to prevent distortion of cell & loss of morphology,
H.
The act of preparing & staining diagnostic slides
I.
To distinguish cellular components by obtaining highly detailed chromatin, differential counterstaining, & cytoplasmic transparency
J.
Deteriorates rapidly, if can't take to lab immediately use pre-fixative holding solution - alcoholic saline or Saccomanno fluid = vol. of specimen
K.
Orange G dH2O phosphotungstic acid (mordant) 95%ethanol glacial acetic acid (opt.) (enhance specificity, decr. stain time)
L.
To remaining sediment add 95% ethanol, denatured alcohol, or 10% NBF, mix by inversion, fix 15-30 min, centrifuge 10 min, 2,000 rpm, pour off supernatant, mass in cassette, may wrap w/ lens paper, if large submit portion, formalin process routinely
M.
Examination of cellular material for disease, esp. cancer
N.
Can process, section, stain, as histo section, easier special stain, incl. IHC, made of material left after smear, make from tissue fragments, clots, heavy mucus, "block" together w/ thrombin-prothrombin clot, albumin, or agar
O.
Scrapings, brushings, viral inclusion smears, use nickel method
P.
Specimens w/ brush head in vial w/ very weak ethanol preservative, cell enrichment - centrifuge conc. abnormal cells, remove blood/mucus, specimen aliquot sediments on slides, nongynecological req. alt. suspension & centrifugation, higher yield cells
Q.
Don't fix, refrigerate immediately
R.
Prepare smear at time of collection, nickel smear, spray-fix, don't let air-dry
S.
Can obscure morphology or outnumber diagnostic cells, crosshatch can help separate cells, dip in Carnoy's or Clark to lyse rbc, commercial products can lyse rbc (maybe diagnostic too)
T.
Send entire amount collected to lab, good for cell block
Type the Question that corresponds to the displayed Answer.
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21.
Sputum & thick bronchial washings, use crush method
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22.
Commerical cytology sprays - usu. ethanol w/ acetone & polyethylene glycol (remove latter w/ alcohol before staining), 95% denatured alcohol, 80% isopropanol (b/c more shrinkage than ethanol), 100% methanol (b/c less shrinkage than ethanol)
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23.
Pleural, peritoneal, pericardial, ascites, effusions, urine, cyst fluids, washings, first centrifuge to concentrate cells, use pull-apart, or cross-hatch method
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24.
Smears fixed w/i 1-2 seconds of application to slide, thick smears/smears w/ mucus may take 2-4 seconds, liquid suspensions longer, air-drying causes nuclear swelling, distortion, loss of cytoplasmic density, cytoplasm becomes eosinophilic
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25.
Mash material between two slides to give thin, even smear, may use back & forth "slap" motion to break chunks, fix w/ 95% ethanol or spray-fixative
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26.
Specimens w/o brush head in vial w/ weak methanol preservative, homogenize & filter, filter pressed on slide to deposit cells, slide can be stained traditionally, nongynecological specimens need extra suspension & centrifugation
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Breast, thyroid, lung, liver, pancreas, cell-laden fluid removed from site, initially use pull-apart method & fix, never feathered-edge, rinse needle w/ physiologic saline (don't rinse w/ fixative) to recover remaining specimen
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28.
hematoxylin ammonia water, or Scott tap water OG-6 EA
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29.
Liquid-based cytology rather than direct smear - better for automated systems - ThinPrep system & SurePath system, smeared on slide, fixed while wet, dry & stain
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30.
2-3 loopfuls of sediment on slide, spread diagonally, back & forth, corner to corner, fix immediately w/ 95% ethanol or spray fixative, if sediment beads form, spread again for monolayer of cells, good for cellular body fluids, esp. w/ lots of blood

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