ASCP Histology Prep: STAINING
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| show | differentiating in 95% alcohol or in acid-alcohol and restain
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| stains that detect fungi | show 🗑
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| what color is a positive result for H. pylori using the Giemsa stain? | show 🗑
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| show | RED
(neg-red)
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| show | Kinyoun/
Fite/
Ziehl Neelsen/
Auramine-Rhodamine
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| H. pylori detection stains are | show 🗑
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| show | Mycobacteria
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| show | blue
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| show | BLACK
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| What step must be added to the fite acid fast stain? | show 🗑
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| show | Fite acid-fast stain
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| What are the steps of the acid fast stain procedure? | show 🗑
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| What color does H.Pylori stain using Diff Quik? | show 🗑
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| what are the steps of the gram stain? | show 🗑
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| What color do GRAM POSITIVE organisms stain using Gram stain? | show 🗑
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| show | red
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| What does Gram stain differentiate between? | show 🗑
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| show | black
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| show | Steiner/Wathin-Starry
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| show | BLACK
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| show | melanin
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| show | carbon/iron/lipochromes
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| What pigment can bind silver? | show 🗑
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| 3 most common artifactual pigments | show 🗑
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| show | melanin/hemosiderin/urates/bile/lipofuchsin
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| show | blue/black nuclei; RED COLLAGEN
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| PAS is used for ________ mucosubstances | show 🗑
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| In metachromatic staining, ___ can be varied to differ acid mucins | show 🗑
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| To suppress background staining in Congo Red procedure, _________________ is used. | show 🗑
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| Alcian Blue/ PAS is used to differentiate between ________. | show 🗑
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| show | formalin
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| show | shows sites in the tissue where the glycogen was removed (digested).
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| show | Prussian blue reaction.
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| show | Mycobacterium (RED)
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| show | 9 parts water adn one part stock solution formalin.
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| show | EM labs/Methanol
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| show | methanol
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| cross links quickly but binds slowly | show 🗑
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| show | 6.0
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| greatest binding action of formaldehyde occurs at ____ to ___ pH | show 🗑
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| show | inside the fixed and stabilized proteins.
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| show | Formaldehyde Saline: isotonic but may produce formalin pigment
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| show | Calcium Formaldehyde: for PHOSPHOLIPIDS
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| show | Acetate Formaldehyde: for Phospholipids but cases PSEUDOCALCIFICATION
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| show | 10% Neutralized Formalin: used widely but becomes acidic almost immediately.
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| Contains Sodium Phosphate monobasic & dibasic/DI/Formaldehyde | show 🗑
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| show | Modified Millonig: used in EM on long stored tissue
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| contains Absolute Ethyl Alcohol/DI/Formaldehyde | show 🗑
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| Formalin PEL = 0.75ppm in __ hours | show 🗑
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| show | 15 minutes
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| Contains Sodium Phosphate monobasic/25% glutaraldehyde/DI | show 🗑
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| Coagulant, produces shrinkage, additive, black pigment NOT preventable | show 🗑
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| show | Osmium tetroxide
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| show | Gridley fungus stain
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| show | Osmium tetroxide
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| show | Picric Acid (avoid for DNA and RNA)
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| non-coagulant, great for MITOCHONDRIA identification procedures | show 🗑
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| replacement for mercury, preserves antigenicity, may replace NBF, makes formalin pigment when unbuffered, superior nuclear detail | show 🗑
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| show | B5
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| mercuric chloride/formaldehyde/sodium acetate (formalin pigment) | show 🗑
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| show | Bouin
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| show | Bouin
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| 95% alcohol w/picric acid/formaldehyde/glacial acetic acid | show 🗑
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| alcoholic Boiun derivative which is great for carbohydrates (GLYCOGEN)remove excess picric with 80% alcohol | show 🗑
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| show | HOLLANDE
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| show | HOLLANDE
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| show | formalin pigment, when the ph is lowered or if solution is unbuffered.
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Created by:
alexinem
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