Detection Methodologies - Quiz 3 and 4
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| Optical Detection | Light scattered by a cell is converted to an electrical pulse.
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| Spectrophotometry | measures light transmitted by a solution to determine concentration with regard to wavelenght
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| Chemilluminescence | Process of generating photons of light via an oxidative reaction in which there is a rapid increase of emitted light and the gradual decay
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| Flame photometry | measures excited atoms to determine concentration of Na+, K+, and Li+
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| Fluorometry | Light is absorbed by the substance and then light of a shorter wavelength is emitted (fluoresced)
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| Nephelometry | Determines concentration by scattering of light at 90 degree angle
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| Atomic Absorption | measures concentration by absorption of electromagnetic radiation by atoms
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| Electrophoresis | Migration of charged solutes or particles in an electrical field
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| Potentiometry | Electrochemical cell consisting of two linked electrodes
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| Chromatography | Used to separate complex mixtures with a mobile phase and stationary phase
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| Electrical Impedence | Particles suspended in an electrolyte solution are made to pass through a small aperture through which an electrical current path has been established
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| Flow Cytometry | means of measuring certain physical and chemical characteristics of cells or particles as they travel in suspension one by one past a sensing point (laser light)
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| Photo-Optical Endpoint | Change in optical density of test sample indicates clot formation
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| Mechanical Endpoint Detection | Detects change in electrical conductivity indicates clot formation
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| Photometry | Measures light intensity without regard to wavelength
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| Turbidometry | a method for determining concentration of a substance in a solution by the degree of cloudiness
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| Mass Spectrophotometry | Substance is bombarded by electrons to form charged molecular ions and fragments.
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Created by:
ckozlak
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