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CLLS-203

Detection Methodologies - Quiz 3 and 4

QuestionAnswer
Optical Detection Light scattered by a cell is converted to an electrical pulse.
Spectrophotometry measures light transmitted by a solution to determine concentration with regard to wavelenght
Chemilluminescence Process of generating photons of light via an oxidative reaction in which there is a rapid increase of emitted light and the gradual decay
Flame photometry measures excited atoms to determine concentration of Na+, K+, and Li+
Fluorometry Light is absorbed by the substance and then light of a shorter wavelength is emitted (fluoresced)
Nephelometry Determines concentration by scattering of light at 90 degree angle
Atomic Absorption measures concentration by absorption of electromagnetic radiation by atoms
Electrophoresis Migration of charged solutes or particles in an electrical field
Potentiometry Electrochemical cell consisting of two linked electrodes
Chromatography Used to separate complex mixtures with a mobile phase and stationary phase
Electrical Impedence Particles suspended in an electrolyte solution are made to pass through a small aperture through which an electrical current path has been established
Flow Cytometry means of measuring certain physical and chemical characteristics of cells or particles as they travel in suspension one by one past a sensing point (laser light)
Photo-Optical Endpoint Change in optical density of test sample indicates clot formation
Mechanical Endpoint Detection Detects change in electrical conductivity indicates clot formation
Photometry Measures light intensity without regard to wavelength
Turbidometry a method for determining concentration of a substance in a solution by the degree of cloudiness
Mass Spectrophotometry Substance is bombarded by electrons to form charged molecular ions and fragments.
Created by: ckozlak