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CLLS-203
Detection Methodologies - Quiz 3 and 4
Question | Answer |
---|---|
Optical Detection | Light scattered by a cell is converted to an electrical pulse. |
Spectrophotometry | measures light transmitted by a solution to determine concentration with regard to wavelenght |
Chemilluminescence | Process of generating photons of light via an oxidative reaction in which there is a rapid increase of emitted light and the gradual decay |
Flame photometry | measures excited atoms to determine concentration of Na+, K+, and Li+ |
Fluorometry | Light is absorbed by the substance and then light of a shorter wavelength is emitted (fluoresced) |
Nephelometry | Determines concentration by scattering of light at 90 degree angle |
Atomic Absorption | measures concentration by absorption of electromagnetic radiation by atoms |
Electrophoresis | Migration of charged solutes or particles in an electrical field |
Potentiometry | Electrochemical cell consisting of two linked electrodes |
Chromatography | Used to separate complex mixtures with a mobile phase and stationary phase |
Electrical Impedence | Particles suspended in an electrolyte solution are made to pass through a small aperture through which an electrical current path has been established |
Flow Cytometry | means of measuring certain physical and chemical characteristics of cells or particles as they travel in suspension one by one past a sensing point (laser light) |
Photo-Optical Endpoint | Change in optical density of test sample indicates clot formation |
Mechanical Endpoint Detection | Detects change in electrical conductivity indicates clot formation |
Photometry | Measures light intensity without regard to wavelength |
Turbidometry | a method for determining concentration of a substance in a solution by the degree of cloudiness |
Mass Spectrophotometry | Substance is bombarded by electrons to form charged molecular ions and fragments. |