CLS 346 Exam 2
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List the two major groups of nitrogen containing compounds | show 🗑
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Convert Urea to a BUN, and BUN to Urea. | show 🗑
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Describe the synthesis of urea in the body | show 🗑
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What three conditions will have increased serum BUN | show 🗑
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What is the reason for the usage of the BUN/Creatinine ratio? | show 🗑
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show | BUN is diminished. Creatinine is normal. -> acute renal tubular necrosis, malnutrition, low protein diet, or over hydration.
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What conditions will have a high BUN/Creatinine ratio? | show 🗑
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State the basic principle of enzymatic BUN methodologies | show 🗑
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Briefly describe the synthesis of uric acid in the body: | show 🗑
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show | Elevation of uric acid greater than 7.0 mg/dl
Gout: monosodium urate forms in the joints and tissues, patients with carcinomas, arthritic attack.
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Define Hypouricemia and give a cause of the condition: | show 🗑
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show | based on the reaction of uric acid with PTA to form tungsten blue in an alkaline medium. Bi-product formed is hydrogen peroxide.
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show | uricase acts on uric acid to produce allantoin, hydrogen peroxide, and CO2. The reaction is measured in kinetic or equilibrium mode.
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State the basic principle for the HPLC method of uric acid methodology | show 🗑
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Describe the formation of Creatinine in the body | show 🗑
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List two conditions that lead to increased Creatinine levels in blood serum | show 🗑
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show | Jaffe reaction: Creatinine reacts with picrate in alkaline medium to yield orange-red complex
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State and explain the enzymatic method used in Creatinine determination | show 🗑
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