AMST-antimicrobial
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| What are the two main functions of microbiology laboratory? | Diagnostic and Therapeutic
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| What is the definition of diagnostic, in terms of microbiology lab? | Detection, isolation, and identification of agents and or immunoassay (serology)
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| What is the definition of therapeutic in terms of microbiology lab? | antimicrobial susceptibility testing as a guide to appropriate therapy
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| What is the fundimental principle of antimicrobials and antimicrobial therapy? | Selective toxicity
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| What are three categories of antimicrobials? | antibacterial, antifungal, antiviral
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| What is meant by the optimal agent? | the one with the narrowest spectrum of activity, fewest side effects, and lowest toxicity
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| What are seven things that a physician must consider when treating an individual patient? | site of infection
age
status of liver and kidney
pregnancy
hypersensitivity
idiosyncrasy
drug interactions
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| What are four disadvantages to improper us of antimicrobials? | toxicity
hypersensitivity
alteration of indigenous flora
selection of resistant strains
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| What are the three purposes of antimicrobial susceptibility testing? | initiation of appropriate therapy
modification of existing therapy
determination of susceptibility patterns in local environment
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| What are the methods that can be used in antimicrobial susceptibility? | Broth dilution
Agar diffusion
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| What does broth dilution yield? | MIC value
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| What does agar diffusion yield? | SIR value
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| What is an Antibiogram? | a species susceptibility pattern
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| What is an antibiogram useful for? | guiding to an empirical therapy
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| Kirby-Bauer is what kind of method used in antimicrobial susceptibility? | Agar diffusion
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| What does SIR stand for? | Susceptible/Intermediate/Resistant
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| What do you use to determine the SIR? | the diameter of the zone of inhibition
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| What unit of measurement used in the zone of inhibition? | mM
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| What does it mean if there is a colony within the zone of inhibition? | There is a sub colony that is resistant to that antimicrobial
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| A zone has a fuzzy edge, what should be measured? | just the clear edge, not the fuzzy area
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| MIC is used to determine what? | the lowest concentration of drug that inhibits growth
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| When using a micro broth dilution for susceptibility testing, what do you look for to determine growth? | Cell button on bottom
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| If there is no cell button on the micro broth dilution susceptibility test, what can be concluded? | There was no growth or the microbe is susceptible to that concentration of the antimicrobial
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| What kind of agar is used in the kirby-bauer test? | Meuller hinton agar
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| When interpreting SIR what does it mean if a microbial is S? | MIC
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| When interpreting SIR what does it mean if a microbial is R? | MIC > achievable level by usual dosage and schedule
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| What does MIC stand for? | Minimal Inhibitory Concentration
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| When interpreting SIR what does it mean if a microbial is I? | MIC >= expected level at site of infection by usual dosage and schedule, may respond to high dosage
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| Why is it important to take note of a intermediate? | when there is no susceptible options
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| What is pseudomonus notorious for? | Naturally resistant
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Created by:
jnwells03
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