exam3
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
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| Entamoeba hartmanni | "small histolytica
stool specimen
troph- 1 nucleus, central karyosome
cytoplasm- fine granular
cyst- 4 nuclei, elongated chromatoid bodies, rounded ends
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| Endolimax nana | stool specimen
troph-1 nucleus, blot like karyosome
cyst- 4 nuclei, blot like karyosome
no chromatoid bodies
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| Lodameoba buetschlii | stool specimen
troph-1 nucleu;, large, central karyosome, retractile granules
cytoplasm- coarsely ground, vaculated
cyst- 1 large nucleus, eccentric karyosome, compact, well defined glycogen mass
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| Entamoeba polecki | stool spec
troph- 1 nucleus, small discrete karyosomal chromatin
cytoplasm- coarsely granular, vaculated
cyst- 1 nucleus, sm eccentric karyosome(pleomorphic)
cytoplasm - small inclusions/mass
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| Retortamonas intestinales | rare cyst - 1 nuclei, fibrils shaped like "birds beak" troph- slightly pear shaped or oval, 1 nucleus; cytostome extends half way; 2 flagella, posterior/anterior
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| Dientamoeba fragilis | found in mucosal lining of lg intestines; unclear life cycle usually asymptomatic/diarrhea, abdominal pain no cyst troph- binuclear/1
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| 4 morphologic forms of HEMOFLAGELLATES | amastigote promastigote epimastigote trypomastigote
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| amastigote | round, 1 nucleus, kinetoplast dot-like blepharoplast, gives rise to axomneme single parabassal body, adjacent to blepharoplast no flagella
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| promastigote | long, slender body with free flagella 1 lg nucleus near center kinetoplast is anterior to nucleus rise to axoneme and flagella
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| epimastigote | body is long, wider 1 lg nucleus, posterior kinetoplast anterior to nucleus undulating membrane half way into free flagella
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| trypomastigote | C/U shape on stained blood slides; curve around RBCs long, slender body posterior kinetoplast w/ undulating membrane whole length of body flagella whole length
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| specimens of choice for hemoflagellates | blood lymph node/ulcer aspirations bone marrow tissue biopsies CSF
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| Leshmania braziliensis | specimen- biopsy of infected ulcer diagnostic stage- amastigote found Brazil, Columbia, Ecuador, Venezuela, Peru vector - Lutzomyia/psychodopygus sandfly res
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| Mucocutaneous Leishmaniasis | infection of Leshmania braziliensis large ulcers in oral/nasal mucosa; destruction of nasal septum
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| Leishmania donovani chagasi Leishmania donovani donovani Leishmania donovani infantum | Middle East vector - sandfly reservoirs- chagasi-dog, cat, fox donovani- none/fox infantum- dog, fox, jackal, porcupine
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| Kalaazar or dum dum fever | Visceral leishmaniasis enlarged liver/spleen; darkening of skin "black fever"
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| Leishmania tropica group | L. aethiopica L. major L. tropica
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| Leishmania tropica vector; attacks what human organ; reservior; infectious phase | Phlebotomus sandfly; lymphoid tissue of skin; possibly dogs; amastigote
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| Leishmania aethiopica reservior | rock hyrax
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| Leishmania major reservior | gerbils; rodents
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| "Old World Leishmaniasis"; "Oriental Sore"; "Baghdad/Delhi boil" | L. Tropica; Cutaneous Leishmaniasis; develops a small red papule with intense itching
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| Diffuse cutaneous leishmaniasis (DCL) | immune system fails and self-healing doesn't occur
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| Trypanosoma brucei gambiense | vector: Glossina palpalis/tachinoides tsetse flies humans infected by trypomastigote (diagnositic); travels to lymph system then CNS multiply by binary fission found in West/Central Africa
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