Infusion/AUTOLOGOUS Stem Cells
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| What forms are already in the folder? | 1.Request for Cellular Therapy Processing and Infusion
2. Cumulative report (of units that are acceptable)
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| Next important step: | Get out the cellular therapy product release form (one for each BM#)
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| What is the cellular therapy product release form? | Your checklist -- a job aid.
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| Next important step: | Page the designated NP (consult schedule).
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| Next important step: | Clean H2O bath, equilibrate to 37.
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| At the same time: | Put 2.3 inches of N2 in Dewar flask (NOT above cardboard).
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| Next important form: | Cellular Therapy Processing Infusion Record, for floor (see attached)
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| Get out the card and: | find location of product and retrieve; but just put on top in freezer, to rest in vapor phase
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| What label will probably be not that helpful in an autologous HPC (A)? | biohazard label
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| when ready for infusion, do this (3 steps): | 1. put product in Dewar flask 2. sign out in log AND on card ... NOTE TIME! 3. go to floor
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| Once on the floor (3 steps) -- | 1. stamp two forms
2. PLUG IN H2O BATH, GET TO 37∘ (record on form)
3. Fill out as much as possible (stay to fill out remainder)
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| Process begins. First step? | Thaw in sterile, closed bag; edges first, do the bags with segments LAST. Keep seal of bag above water line. If there's a leak, clamp port immediately -- NP will use syringe to withdraw product from either original bag or containing bag.
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| What information is involved in reading? | Patient name, MR #, spell out first and last name; then sign form (wait for even the last one!); record infusion start time
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| On the last unit(s): | Retrieve segments
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| Don't leave without: | the yellow copy
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| What to do with segment: | CBC (first), viability (second; but vortex after each of three washes in Hank's buffered salt solution, and spin for 60 seconds; add a little before bringing to flow)
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| PAPERWORK! First step: (this is a trick question) | Numbers on form in database form "Post-thaw Recovery" -- "pre-freeze TNC" is actually the post-processing TNC ... and on this form, do NOT change the E's. One form per BM number!!! in wfm.b dir.
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| PAPERWORK! (another trick question): total volume of post-thaw? | IMPORTANT: This includes the volume of DMSO added AND the volume **NOT** transfused (total volume frozen)
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| PAPERWORK! (another trick question) ... next question, post-thaw NC/ml | Your white count on the segment. Put in database as E6, database will move the decimal point)
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| What is then calculated for you? | TNC and %NC recovery
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| Important step ... what to do before you leave this form? | Print and save -- replace first part of name with BM #, and put in patient's folder
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| Second to the last step: | In the database, do transplant (UNDER FORMS)
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| Last step: | In computer, remove from freezer file the unit(s) that were/was transfused. BMARROW/LN2 FREEZERS
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Created by:
alanh1954
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