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Infusion/Stem Cells
Infusion/AUTOLOGOUS Stem Cells
| Question | Answer |
|---|---|
| What forms are already in the folder? | 1.Request for Cellular Therapy Processing and Infusion 2. Cumulative report (of units that are acceptable) |
| Next important step: | Get out the cellular therapy product release form (one for each BM#) |
| What is the cellular therapy product release form? | Your checklist -- a job aid. |
| Next important step: | Page the designated NP (consult schedule). |
| Next important step: | Clean H2O bath, equilibrate to 37. |
| At the same time: | Put 2.3 inches of N2 in Dewar flask (NOT above cardboard). |
| Next important form: | Cellular Therapy Processing Infusion Record, for floor (see attached) |
| Get out the card and: | find location of product and retrieve; but just put on top in freezer, to rest in vapor phase |
| What label will probably be not that helpful in an autologous HPC (A)? | biohazard label |
| when ready for infusion, do this (3 steps): | 1. put product in Dewar flask 2. sign out in log AND on card ... NOTE TIME! 3. go to floor |
| Once on the floor (3 steps) -- | 1. stamp two forms 2. PLUG IN H2O BATH, GET TO 37∘ (record on form) 3. Fill out as much as possible (stay to fill out remainder) |
| Process begins. First step? | Thaw in sterile, closed bag; edges first, do the bags with segments LAST. Keep seal of bag above water line. If there's a leak, clamp port immediately -- NP will use syringe to withdraw product from either original bag or containing bag. |
| What information is involved in reading? | Patient name, MR #, spell out first and last name; then sign form (wait for even the last one!); record infusion start time |
| On the last unit(s): | Retrieve segments |
| Don't leave without: | the yellow copy |
| What to do with segment: | CBC (first), viability (second; but vortex after each of three washes in Hank's buffered salt solution, and spin for 60 seconds; add a little before bringing to flow) |
| PAPERWORK! First step: (this is a trick question) | Numbers on form in database form "Post-thaw Recovery" -- "pre-freeze TNC" is actually the post-processing TNC ... and on this form, do NOT change the E's. One form per BM number!!! in wfm.b dir. |
| PAPERWORK! (another trick question): total volume of post-thaw? | IMPORTANT: This includes the volume of DMSO added AND the volume **NOT** transfused (total volume frozen) |
| PAPERWORK! (another trick question) ... next question, post-thaw NC/ml | Your white count on the segment. Put in database as E6, database will move the decimal point) |
| What is then calculated for you? | TNC and %NC recovery |
| Important step ... what to do before you leave this form? | Print and save -- replace first part of name with BM #, and put in patient's folder |
| Second to the last step: | In the database, do transplant (UNDER FORMS) |
| Last step: | In computer, remove from freezer file the unit(s) that were/was transfused. BMARROW/LN2 FREEZERS |
Created by:
alanh1954
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