Review for Microbiology II Test 1
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
|
|
||||
|---|---|---|---|---|---|
| Erythromycin | Macrolide/ Bacteriostatic/Protein synthesis/Gram+/Efflux, Altered target
🗑
|
||||
| Gentamicin | Aminoglycoside/Bactericidal/Protein synthesis/Gram+, Gram-, Not anaerobic/Enzymatic destruction, Altered target, Decreased uptake
🗑
|
||||
| Cefazolin | Beta-lactam/ Bactericidal/Cell wall/Gram+, Gram-, Not anaerobic/ Enzymatic desctruction, Altered target
🗑
|
||||
| Vancomycin | Glycopeptide/ Bactericidal/Cell Wall/Gram+/Altered target
🗑
|
||||
| Levofloxacin | Quinolone/Bactericidal/DNA synthesis/Gram+, Gram-/Decreases uptake, Altered target
🗑
|
||||
| Imipenem | Intravenous β-lactam or carbapenems/ Bactericidal/Cell wall/Gram+, Gram-/ Enzymatic desctruction, Altered target
🗑
|
||||
| Ampicillin | Beta-lactam/ Bactericidal/Cell wall/Gram+, Gram-, Not anaerobic/Enzymatic desctruction, Altered target
🗑
|
||||
| Piperacillin/Tazobactam combo | Beta-lactam..Beta lactamase inhibitor/ Bactericidal/Cell wall/Gram+, Gram-/Enzymatic destruction has been elliminated
🗑
|
||||
| Gram stain morphology of Staphylococcus | Gram-positive cocci in clusters
🗑
|
||||
| Common media for growth of Haemophilius species | Chocolate
🗑
|
||||
| Maconkey, XLD, and HE are best described as what type of media | Selective and differential
🗑
|
||||
| What is the gram stain morphology of Bacillu anthracis | Gram-positive rods
🗑
|
||||
| What is the order of stains in the Gram Stain procedure | Crystal violet, Grams iodine, Decolorizor, Saffrinin
🗑
|
||||
| A result of R/A on LIA agar will be interpreted as | Lysine deamination-red slant/glucose fermentation-yellow butt. No color change in the butt=no glucose fermentation or if lysine decarboxilase is formed in the butt, it reverts back to purple.
🗑
|
||||
| 2 factors necessary for the growth of Haemophilus influenzae | X=Hemin V=NAD
🗑
|
||||
| Gram-negative rod is oxidase +, grows on BAP and Choc ans is associated with cat scratches | Pasteurella mutocida
🗑
|
||||
| Animal common as reservoir for the transmission of Francisella tularenis | Rabits and rodents
🗑
|
||||
| Single carbohydrate that will be fermented by N. gonrrhoeae | Glucose
🗑
|
||||
| Gram-negative rod that has a bleachy odor and will pit the agar | Eikanella corrodens=Human bite wounds
🗑
|
||||
| Pigment color seen in many strains of Pseudomonas aeruginosa | Green
🗑
|
||||
| The optochin test in used to identify which Strep species | Strep pneumoniae
🗑
|
||||
| A total clearing around the colony on BAP is ----- hemolysis | Beta
🗑
|
||||
| Gramstain morphology of Neisseria meningitidis | Gram-negative diplo cocci
🗑
|
||||
| Give the apperance of E.coli and Proteus on MAC agar | E.coli=pink Proteus=clear
🗑
|
||||
| This aerobic gram-negative rod will produce bright red colonies on most standard media | Serratia
🗑
|
||||
| 2 factors that influence the efficacy of disinfectants | Time and strength
🗑
|
||||
| This curved-gram-negative rod may cause gastrointestinal disease and is associated with eating chicken | Campylobacter
🗑
|
||||
| This process is a means by which all microbial life is destroyed | Sterilization
🗑
|
||||
| Which type of hemolysis is typical of Strep. pneumoniae | Alpha
🗑
|
||||
| This H2S positive gram-negative organism is implicated in food borne illnesses especially eggs and poultry | Salmonella
🗑
|
||||
| Swarming on blood agar would indicate the presence of | Proteus
🗑
|
||||
| Bacteroides fragilis is most likely to be cultivated in this type of atmosphere | Anaerobic
🗑
|
||||
| Two methods for measuring turbidity | Hold the specimen against a white background with black lines or measuring on an instrument
🗑
|
||||
| Two criteria for for selection of antimicrobial agents to be tested | Agent needs to have a known effect on the organism and it needs to be affective in the area or site of the bacterial growth
🗑
|
||||
| What if several colonies of E. coli are too large | Concentration of the inoculum is to small, agar is too thin, Antimicrobial concentration is too large
🗑
|
||||
| What if you have small colonies within the zone of inhibition of Tetracycline with S. aureus | Mixed culture or possible resistant mutations. Verify culture purity.
🗑
|
||||
| Gentamycin zone too small with P.aeruginosa | Ca+ and/or Mg+ level too high in medium. Aquire a new lot of agar medium that will meet QC criteria
🗑
|
||||
| What is the relationship between MIC and zone size | Inversely proportional. The smaller the MIC the larger the zone
🗑
|
||||
| An organism with an increased MIC value sill be expected to have an increased or decreased zone size | Decreased
🗑
|
||||
| Name the three weeky QC organisms used for the Kirby Bauer method | S.aureus ATCC 25293, E. coli ATCC 25922, P. aeruginosa ATCC 27853
🗑
|
||||
| Describe the action of (amoxicillin or ticarcillin)/clavulanic acid combination | Clavulanic acid inactivates a wide range of beta-lactamase enzymes allowing the Ticarcillin to inhibit or kill the organism
🗑
|
||||
| An oxicllin disk is used to test S. pneumoniae of penicillin. Why? | The penicillin test was not sufficiently sensitive to detect subtle but significant changes in suseptability
🗑
|
||||
| What should be done if the oxicillin suseptability test on S pneumoniae is resistant or <= to 20mm | Further testing by MIC method is needed.
🗑
|
||||
| Define emperic antimicrobial therapy | Hitting the organism with a broad spectrum antimicrobial before a definitive ID is made
🗑
|
||||
| Norofloxacin and Nitrofurantoin are specific for | Urines
🗑
|
||||
| Criteria for anitmicrobial battery content and use | Organism ID or group, Acquired resistance patterns common to local microbial flora, antimicrobial susceptibility testing method used, site of infection, avalability of antimicrobial agents in formulary
🗑
|
||||
| Susceptibility | Indicates that the antimcrobial agent in question may b an appropriate choice for treating the infection. Bacterial resistance is absent or at a clinically insignificant level
🗑
|
||||
| Intermediate | A number of possibilities: May still be effective but perhaps not as much as another choice. May be effective at a particular site of infection.An interpretive safety margin
🗑
|
||||
| Resistant | Indicates that the antimicrobial agent may not be am appropriate choice, either because the organism is not inhibited by serum-achievable levels or because test results highly correlate with resistance
🗑
|
||||
| AST commonly required for: | Staph, Step. pneumo, Viridans streptococci, Entrococci, Entrobacteriaceae,Pseudomonas aeruginosa, Acinetobacter spp.
🗑
|
||||
| AST occasionally required for: | Haemophilius influenzae, Neisseria gonorrhoeae, Moraxella catarrhalis, Anearobic bacteria
🗑
|
||||
| AST rarely required for: | Beta-hemolytic streptococci (groups A, B, C, F and G), Neisseria meningitides, Listeria monocytogenes
🗑
|
||||
| Requiring further Eval: Staphylococci | Vancomycin intermediate or resistant, Clindamycin resistant; erythromycin susceptible, Linezolid resistant, Daptomycin resistant
🗑
|
||||
| Viridans streptococci | Vancomycin intermediate or resistant
🗑
|
||||
| Strep. pneumo | Vancomycin intermediate or resistant
🗑
|
||||
| Beta-hemolytic strep. | Penicillin intermediate or resistant
🗑
|
||||
| Enterobacteriacea | Imipenem resistant
🗑
|
||||
| Entrobacter/Citrobacter/Serratia/Morganella/Providencia | Susceptible to ampicillin of cefazolin
🗑
|
||||
| Pseudomonas aeruginosa | Amikacin resistant; gentamicin or tobramycin susceptable
🗑
|
||||
| Stenotrophomonas maltaphilia | Imipenem susceptable; trimethoprim/sulfamethoxazole resistant
🗑
|
||||
| Neisseria gonorrheae | Ceftriaxone resistant
🗑
|
||||
| Neisseria meningitidis | Penicillin resistant
🗑
|
Review the information in the table. When you are ready to quiz yourself you can hide individual columns or the entire table. Then you can click on the empty cells to reveal the answer. Try to recall what will be displayed before clicking the empty cell.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
Created by:
1414395397
Popular Laboratory Science sets