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MicroBio II Test 1

Review for Microbiology II Test 1

Erythromycin Macrolide/ Bacteriostatic/Protein synthesis/Gram+/Efflux, Altered target
Gentamicin Aminoglycoside/Bactericidal/Protein synthesis/Gram+, Gram-, Not anaerobic/Enzymatic destruction, Altered target, Decreased uptake
Cefazolin Beta-lactam/ Bactericidal/Cell wall/Gram+, Gram-, Not anaerobic/ Enzymatic desctruction, Altered target
Vancomycin Glycopeptide/ Bactericidal/Cell Wall/Gram+/Altered target
Levofloxacin Quinolone/Bactericidal/DNA synthesis/Gram+, Gram-/Decreases uptake, Altered target
Imipenem Intravenous β-lactam or carbapenems/ Bactericidal/Cell wall/Gram+, Gram-/ Enzymatic desctruction, Altered target
Ampicillin Beta-lactam/ Bactericidal/Cell wall/Gram+, Gram-, Not anaerobic/Enzymatic desctruction, Altered target
Piperacillin/Tazobactam combo Beta-lactam..Beta lactamase inhibitor/ Bactericidal/Cell wall/Gram+, Gram-/Enzymatic destruction has been elliminated
Gram stain morphology of Staphylococcus Gram-positive cocci in clusters
Common media for growth of Haemophilius species Chocolate
Maconkey, XLD, and HE are best described as what type of media Selective and differential
What is the gram stain morphology of Bacillu anthracis Gram-positive rods
What is the order of stains in the Gram Stain procedure Crystal violet, Grams iodine, Decolorizor, Saffrinin
A result of R/A on LIA agar will be interpreted as Lysine deamination-red slant/glucose fermentation-yellow butt. No color change in the butt=no glucose fermentation or if lysine decarboxilase is formed in the butt, it reverts back to purple.
2 factors necessary for the growth of Haemophilus influenzae X=Hemin V=NAD
Gram-negative rod is oxidase +, grows on BAP and Choc ans is associated with cat scratches Pasteurella mutocida
Animal common as reservoir for the transmission of Francisella tularenis Rabits and rodents
Single carbohydrate that will be fermented by N. gonrrhoeae Glucose
Gram-negative rod that has a bleachy odor and will pit the agar Eikanella corrodens=Human bite wounds
Pigment color seen in many strains of Pseudomonas aeruginosa Green
The optochin test in used to identify which Strep species Strep pneumoniae
A total clearing around the colony on BAP is ----- hemolysis Beta
Gramstain morphology of Neisseria meningitidis Gram-negative diplo cocci
Give the apperance of E.coli and Proteus on MAC agar E.coli=pink Proteus=clear
This aerobic gram-negative rod will produce bright red colonies on most standard media Serratia
2 factors that influence the efficacy of disinfectants Time and strength
This curved-gram-negative rod may cause gastrointestinal disease and is associated with eating chicken Campylobacter
This process is a means by which all microbial life is destroyed Sterilization
Which type of hemolysis is typical of Strep. pneumoniae Alpha
This H2S positive gram-negative organism is implicated in food borne illnesses especially eggs and poultry Salmonella
Swarming on blood agar would indicate the presence of Proteus
Bacteroides fragilis is most likely to be cultivated in this type of atmosphere Anaerobic
Two methods for measuring turbidity Hold the specimen against a white background with black lines or measuring on an instrument
Two criteria for for selection of antimicrobial agents to be tested Agent needs to have a known effect on the organism and it needs to be affective in the area or site of the bacterial growth
What if several colonies of E. coli are too large Concentration of the inoculum is to small, agar is too thin, Antimicrobial concentration is too large
What if you have small colonies within the zone of inhibition of Tetracycline with S. aureus Mixed culture or possible resistant mutations. Verify culture purity.
Gentamycin zone too small with P.aeruginosa Ca+ and/or Mg+ level too high in medium. Aquire a new lot of agar medium that will meet QC criteria
What is the relationship between MIC and zone size Inversely proportional. The smaller the MIC the larger the zone
An organism with an increased MIC value sill be expected to have an increased or decreased zone size Decreased
Name the three weeky QC organisms used for the Kirby Bauer method S.aureus ATCC 25293, E. coli ATCC 25922, P. aeruginosa ATCC 27853
Describe the action of (amoxicillin or ticarcillin)/clavulanic acid combination Clavulanic acid inactivates a wide range of beta-lactamase enzymes allowing the Ticarcillin to inhibit or kill the organism
An oxicllin disk is used to test S. pneumoniae of penicillin. Why? The penicillin test was not sufficiently sensitive to detect subtle but significant changes in suseptability
What should be done if the oxicillin suseptability test on S pneumoniae is resistant or <= to 20mm Further testing by MIC method is needed.
Define emperic antimicrobial therapy Hitting the organism with a broad spectrum antimicrobial before a definitive ID is made
Norofloxacin and Nitrofurantoin are specific for Urines
Criteria for anitmicrobial battery content and use Organism ID or group, Acquired resistance patterns common to local microbial flora, antimicrobial susceptibility testing method used, site of infection, avalability of antimicrobial agents in formulary
Susceptibility Indicates that the antimcrobial agent in question may b an appropriate choice for treating the infection. Bacterial resistance is absent or at a clinically insignificant level
Intermediate A number of possibilities: May still be effective but perhaps not as much as another choice. May be effective at a particular site of infection.An interpretive safety margin
Resistant Indicates that the antimicrobial agent may not be am appropriate choice, either because the organism is not inhibited by serum-achievable levels or because test results highly correlate with resistance
AST commonly required for: Staph, Step. pneumo, Viridans streptococci, Entrococci, Entrobacteriaceae,Pseudomonas aeruginosa, Acinetobacter spp.
AST occasionally required for: Haemophilius influenzae, Neisseria gonorrhoeae, Moraxella catarrhalis, Anearobic bacteria
AST rarely required for: Beta-hemolytic streptococci (groups A, B, C, F and G), Neisseria meningitides, Listeria monocytogenes
Requiring further Eval: Staphylococci Vancomycin intermediate or resistant, Clindamycin resistant; erythromycin susceptible, Linezolid resistant, Daptomycin resistant
Viridans streptococci Vancomycin intermediate or resistant
Strep. pneumo Vancomycin intermediate or resistant
Beta-hemolytic strep. Penicillin intermediate or resistant
Enterobacteriacea Imipenem resistant
Entrobacter/Citrobacter/Serratia/Morganella/Providencia Susceptible to ampicillin of cefazolin
Pseudomonas aeruginosa Amikacin resistant; gentamicin or tobramycin susceptable
Stenotrophomonas maltaphilia Imipenem susceptable; trimethoprim/sulfamethoxazole resistant
Neisseria gonorrheae Ceftriaxone resistant
Neisseria meningitidis Penicillin resistant
Created by: 1414395397



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