M (ASCP) EXAM
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Is widely distributed in the external environment. It may be found in 20 to 40% of the anterior nares of human adults. It also colonizes the skin, particularly in intertriginous areas such as the groin and axilla, and may be found in the vaginal canal | show 🗑
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Was discovered more than 100 years ago by Hans Christian Gram. Crystal violet-primary stain. Gram's iodine-mordant to fix the dye. GP Bacteria retain the dye in cell wall. Acid alcohol-decolorizer. GN bacteria stain red Safranin-counterstain. | show 🗑
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show | Acridine orange
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Catalase, Coagulase, Dnase, PYR, Nonmotile, Salt tolerance all + | show 🗑
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Penicillin and cephalosporin (1st generation) | show 🗑
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Is the most frequently isolated coagulase-negative staphylococci from human sources. | show 🗑
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show | S. epidermidis
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show | Drug of choice for Staphylococcus epidermidis
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Has a particular predilection for causing urinary tract infections in young, healthy, sexually active females. | show 🗑
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show | Staphylococcus intermedius
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Resistance to novobiocin is a key feature used in the presumptive identification of | show 🗑
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show | Streptococcus pyogenes
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Diseases include scarlet fever, rheumatic fever, endocarditis, and nephritis. | show 🗑
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Small transparent colonies/wide zone of beta hemolysis. ID-Low conc of bacitracin (0.04 μg)/PYR +. | show 🗑
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show | Streptococcus agalactiae
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Infections include meningitis, pneumonia, polynephritis, sepsis including endocarditis/in women, puerperal infection assctd w/ abortion and premature labor. Neonatal sepsis and meningitis | show 🗑
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show | S. pyo
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Penicillin, ampicillin, carbenicillin, cephalosporin 1st gen, erythromycin | show 🗑
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show | Group D streptococci
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Can be divided into one of 83 different capsular serotypes(Latex agg, coagg, or the Neufeld quellung rxn)3, 4, 14, and 19, are particularly virulent. The capsular material prevents phagocytosis and killing by the host phagocytic cells. | show 🗑
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Alpha hemolytic and smooth, moist, or mucoid/"checker piece" appearance/ susceptibile to either bile or ethylhydrocupreine hydrochloride (Optochin) | show 🗑
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Lobar type pneumonia and bacterial meningitis in adults, infants, and toddlers. Diabetes and alcoholism are common conditions predisposing to serious pneumococcal infections. | show 🗑
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Is the most important agent of diarrheal disease in humans. | show 🗑
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1."campy" agar to inhibit the overgrowth of the normal intestinal flora. 2.42°C, the optimum temperature for growth of this organism. 3.incubation atmosphere, consisting of 5% oxygen, 10% CO2, and 85% nitrogen. | show 🗑
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show | C. fetus
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Human infections that have been reported virtually always affect an individual who is debilitated, has compromised immune function, has a neoplastic disease, or has a chronic underlying disorder such as renal or hepatic failure. | show 🗑
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show | C. fetus
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show | Drug of choice for C. fetus and C. jejuni
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Is one of several spiral-shaped bacteria that have been observed in gastric secretions. Can be recovered from the GI tract of humans and a variety of domestic and wild animals, including several species of birds. | show 🗑
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show | Helicobacter pylori
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show | Helicobacter pylori
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show | Helicobacter pylori
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Can be suspected when GNDC or D-CB are observed in Gram stains. MacConkey growth (pinkish tinge). Lack of motility, absence of cytochrome oxidase activity, inability to reduce nitrates to nitrites, and resistance to penicillin. | show 🗑
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Nosocomial infections most commonly involve the respiratory tract, the urinary tract, the genital tract, peritonitis in patients receiving continuous peritoneal dialysis, and postsurgical wounds. | show 🗑
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Carbenicillin, Cephalosporin 2nd or 3rd, Gentamicin, Tetracycline, SXT | show 🗑
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GN nonfermenters that grow on Mac, oxidase +, and motile via peritrichous flagella. Synonymous with A. odorans/ apple odor/ green discoloration. asaccharolytic | show 🗑
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Exist in soil and water/ Blood, sputum, and urine are the most common sources and are often associated with nosocomial infections, nebulizers, respirators, and lavage fluids. | show 🗑
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Exist in soil and water/ Blood, sputum, and urine are the most common sources and are often associated with nosocomial infections, nebulizers, respirators, and lavage fluids. | show 🗑
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Pipericillin and tircarcillin clavulanate | show 🗑
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Inhabiting the epithelial cells in the upper respiratory tract only of man, to which the bacterial cells attach, invade, and survive. Highly contagious, transmitted from human to human via contaminated airborne respiratory droplets. | show 🗑
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show | Bordetella pertussis
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Each neutralize the effects of the fatty acids, metalic ions, and peroxidases also contained in the media. Growth require 2 - 4 days/ 35o C. 1-2 mm in diameter, entire, dome-shaped, gray and shiny, resembling drops of mercury. GNCB/ pleomorphic in older c | show 🗑
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Produces cytochrome oxidase and catalase (weakly), is non-motile (possesses no flagella) | show 🗑
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show | Drug of choice Bordetella pertussis
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is the most common cause of human infections and is the most virulent. It is found in the Mediterranean region, Latin America, and Asia. | show 🗑
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show | B. abortus (cattle)
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show | B. suis (swine)
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show | Brucella
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Ciprofloxacin, tetracycline, streptomycin | show 🗑
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Produces yellow-green colonies/ fruity odor,Mac agar, is oxidase +, and asaccharolytic, hydrolysis of DNA, gelatin, and urea, and (-) reactions for indole, nitrate reduction, and hydrolysis of esculin and ONPG/ resistant to polymyxin B. | show 🗑
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show | Flavobacterium odoratum
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Is the species causing human infections, including 2 biovars, ss tularensis (North America), and ss palaearctica (Europe). Reservoirs of this bacterium include rabbits, rodents, squirrels, beavers, deer, and domestic animals | show 🗑
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Transmission between animals is via ticks and biting flies. Humans acquire infection via the bites of infected ticks or deerflies or by direct contact with the tissues of infected animals, as can occur during the skinning and evisceration of game animals. | show 🗑
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Growth on buffered charcoal yeast extract agar (BCYE) and no growth on blood agar is a presumptive clue that an isolate may be | show 🗑
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show | L. pneumophila
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Is the species most commonly associated with classic legionellosis.The most common manifestation is pneumonia. A milder form of the disease, Pontiac fever, presents as an influenza-like syndrome w/out sequelae and with few complications. | show 🗑
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Gray-white, smooth, opaque on BAP and choc, appear as small GNCBPR/ catalase and oxidase +. Asaccharolytic, strong DNase actvty, and the prodtn of beta lactamase/ separated from Neisseria species by hydrolysis of ester-linked butyrate groups (tributyrin) | show 🗑
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show | MTM
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show | N. gonorrhoeae
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show | Neisseria meningitidis
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show | N. lactamica
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show | Oligella urethralis
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The reduction of nitrite (but not nitrate) and + phenylalanine deaminase activity are two characteristics that, when taken together, separate __________ from Moraxella species. | show 🗑
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show | O. ureolytica
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show | O. ureolytica
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The reduction of nitrates n nitrites (most strains) n + phenylalanine deaminase activity separates from CDC group IVc-2. Most human isolates have been from the urine, specifically in patients with long-term in-dwellin catheters | show 🗑
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show | B. cepacia
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Survives in various disinfectants, including povidone-iodine n quaternary ammonium complbs, n in fluids containin cycloheximide. Nosocomial infections pneumonia, septicemia, endocarditis, n septic arthritis, cystic fibrosis. | show 🗑
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Trimethoprim sulfamethoxazole is the drug of choice for the treatment of infections with this organism. | show 🗑
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show | Pseudomonas aeruginosa
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show | Pseudomonas aeruginosa
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Produce H@S, a characteristic helpful in diffn it from C. koseri, which is H2S (-). differentiated from certain closely related Salmonella species by failure to decarboxylate lysine decarboxylase, hydrolyzing ONPG, and the ability to grow in KCN. | show 🗑
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decarboxylates ornithine and produces acid from adonitol and malonate, both of which are negative reactions for C. freundii. In contrast, C. freundii produces acid from melibiose and sucrose, both of which are negative for most strains of C. koseri. | show 🗑
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show | Edwardsiella tarda
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GNe sepsis and endotoxin-induced shock are serious complications. Urinary tract and wound infections, pneumonia in debilitated and immunocompromised patients, and neonatal meningitis are common infections. | show 🗑
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show | Escherichia coli
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show | Enterotoxigenic strains
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produce an illness characterized by fever, malaise, vomiting and diarrhea, primarily in children. | show 🗑
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penetration of the intestinal mucosa by the bacterial cells results in an inflammatory diarrhea similar to that produced by Shigella species. Blood, mucous, and segmented neutrophils are observed in fecal smears. | show 🗑
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chiefly serogroup 0157:H7, produce a toxin that has a cytotoxic effect on Vero cells (called a verotoxin), producin in vivo effects similar to that produced by Shiga toxin. | show 🗑
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show | Enterohemorrhagic strains:
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is the cause of a necrotizing lobar type pneumonia in which there is considerable hemorrhagic necrosis, leading to expectoratn of a "brick red" sputum that, when mixed with mucin, has a "currant jelly" appearance. | show 🗑
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show | K. pneumoniae
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show | K. pneumoniae
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Two key characteristics separating species from certain closely related Enterobacter species are the lack of motility and the inability to decarboxylate ornithine. | show 🗑
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show | Klebsiella oxytoca
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show | Morganella morganii
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The combination characteristics of citrate negative, failure to produce hydrogen sulfide, and decarboxylation of ornithine | show 🗑
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show | Proteus mirabilis
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show | Proteus mirabilis
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show | P. vulgaris
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show | Providencia
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show | Salmonella
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May cause septicemia | show 🗑
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Typhoid fever. Blood + early (1st wk)/ stool + in 2nd/3rd wk | show 🗑
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show | S. arizona
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show | Serratia marcescens
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(D) Most common/ has its natural habitat in the fecal content of humans. Infections occur following ingestion of contaminated food or water/ Lactose -, nonmotile, anaerogenic | show 🗑
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Group A most severe | show 🗑
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Group B | show 🗑
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show | S. boydii
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The spread of black/ bubonic plague is via rodents to fleas, fleas to rodents. | show 🗑
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acute mesenteric lymphadenitis and "pseudotubercules | show 🗑
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show | Y. enterocolitica
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Motile by polar flagella, most are indole +, growth on mac, cellulitis, wound infections and diarrhea | show 🗑
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show | Bacteria
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show | Fungi
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Enzyme synthesis and cell elongation | show 🗑
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show | Log Phase
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Exhaustion of nutrients, less viability | show 🗑
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Dead cells exced new cells | show 🗑
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"Chinese Letters"; Metachromatic granules (Loeffler's Slants); tellurite hydrolysis (tinsdale agar); elek test determines toxin prod | show 🗑
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Tumbling motility at 25C, but not 37C; cold enrichment, neonatal menigitis and sepsis, sepsis in immunocomp hosts | show 🗑
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Test tube brush in gelatin, infection in fisherman, butchers, veterinarians | show 🗑
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show | Bacillus anthracis
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show | B. cereus
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show | Streptobacillus moniliformis
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Peridontal and jaw abscesses; high number in plague, center of colony has 4-6 pointed star | show 🗑
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show | eikenella
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show | capnocytophaga
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similar to actinobacillus, endocarditis | show 🗑
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show | cardiobacterium
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clue cells; 10% koh added to discharge= fishy odor | show 🗑
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require x and v factors | show 🗑
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schoolof fish, genital ulcers | show 🗑
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growth on BCYE, legionaires disease | show 🗑
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inverted fried egg, dienes stain not gram stain | show 🗑
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causes primary atypical pneumonia, cold agglutinin titer | show 🗑
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show | bacteroides fragilis
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gn ana pits agar, urease + | show 🗑
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show | fusobacterium nucleatum
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gn ana, rods variable in length and width | show 🗑
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show | fusobacterium mortiferum
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gp ana, pseudomembranous colitis, CCFA agar, horse stable odor, spore former | show 🗑
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show | c. perfringens
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gp ana,terminal spores, racquet shaped, tetanus | show 🗑
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show | actinomyces israelii
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gp ana, sensitive to sps | show 🗑
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rickettsial pox, house mites | show 🗑
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show | coxiella burnetti
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show | r. prowazekii
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show | r. rickettsiae
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murine typhus, rat flea | show 🗑
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show | mycobacterium tuberculosis
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environmental org, cause pulmonary disease, disseminated disease, infect immunocomp patients, nonpigmented on LJ, id by nucleic acid probes | show 🗑
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show | m. leprae
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show | EIA
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Molecular cloning of a specific DNA sequence/ if viral ab unknown matches clone, the viral id is confirmed | show 🗑
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Method in which nucleic acid seq can be amplified in vitro/ carried out in cycles, each doubling the amount of desired nucleic acid product | show 🗑
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yellow fever, dengue, st. louis encephalitis, mosquito vector | show 🗑
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show | hantavirus
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assoc w/ shellfish; one of most stable viruses infectings humans | show 🗑
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show | Hepatitis c Virus (HAC)
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show | morbillivirus
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poliomyelitis, aseptic meningitis, occurs naturally only in humans | show 🗑
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seriuos respiratory infection in young children, giant multinucleated cells due to fusion of infected cells | show 🗑
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show | rhabdovirus
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common cold | show 🗑
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show | rotavirus
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show | rubivirus
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respiratory infections | show 🗑
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infection mononucleosis, chronic failure syndrome, assc w. burkett's lymphoma,heterophile ab | show 🗑
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show | zygomycota
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show | ascomycota
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septate; sexual, mushrooms,club fungi | show 🗑
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show | deuteromycota
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simialar to SAB, inhibits some candida and crytococcus, aspergilluis fumigatus and pseudallescheria | show 🗑
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show | bird seed agar
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show | corn meal agar
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show | microsporum
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show | microsporum audouinii
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thick walled macroconidia; knobby end | show 🗑
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thin walled macroconidia | show 🗑
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mostly in adults; hair skin and nails; no fluorescing hairs | show 🗑
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urease +. rose-brown reverse | show 🗑
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show | trichophyton rubrum
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show | trichophyton tonsurans
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show | epidermophyton floccosum
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show | histoplasma capsulatum
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along oh, ms valley n appalachia, may cause skin lesions, yeast (broad based bud, double-contoured wall), mycelial (lollipop forms) | show 🗑
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show | coccidioides immitis
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show | paracoccidioides brasiliensis
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found in dirt and on plants (rose gardener's mycosis), yeast (cigar bodies), mold (delicate hyphae with ovoid conidia along side or in rosettte heads) | show 🗑
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germ tube +, urease -, may be isolated in blood of immunosuppressed | show 🗑
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show | candida tropicalis
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show | geotrichum
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budding from both corners of arthroconidia urease + | show 🗑
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assimilates only glucose and trehalose, no pseudohyphae | show 🗑
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show | cryptococcus neoformans
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show | candida sp. or sporothrix schenckii
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show | histoplasma capsulatum
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show | candida sp.
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large yeast with broad based buds | show 🗑
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large yeast with multiple buds | show 🗑
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show | coccidioides immitis
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green or blue-green colonies; branching or penicillus head, sterigmata blunt | show 🗑
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show | acremonium
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show | fusarium
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show | aspergillus
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green conidia | show 🗑
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yellow conidia | show 🗑
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show | aspergillus niger
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Dog hookworm | show 🗑
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show | ancylostoma duodenale
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show | ascaris lumbricoides
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show | clonorchis sinensis
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broad fish tapeworm | show 🗑
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hydatid tapeworm | show 🗑
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pinworm | show 🗑
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show | fasciolopsis buski
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show | fasciola hepatica
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show | hymenolepis diminuta
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show | hymenolepsis nana
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eyeworm | show 🗑
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show | necator americanus
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blinding worm | show 🗑
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show | paragonimus westermani
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bladder fluke | show 🗑
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show | schistosoma japonicum
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show | schistosoma mansoni
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show | stronglyloides stercoralis
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show | taenia saginata
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show | taenia solium
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dog/cat ascarid | show 🗑
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whipworm | show 🗑
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