HISTOLOGY
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| 1. THE PURPOSE OF A FIXATIVE IS TO | Harden tissue for further processing
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| 2. THE TERM DENATURE APPLIES TO | Protein
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| 3. A "PLANOCONCAVE" MICROTOME KNIFE MAY BE DEFINED AS: | Having one side concave and one side straight
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| 4. THE PROPER ORDER OF TISSUE PROCESSING IS: | Fixation, decalcification, dehydration, clearing and embedding
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| 5. The clearing stage of tissue processing: | Is an intermediate step between alcohols and paraffin.
Is an intermediate step between dehydrating reagents and embedding media
Can be dne with exylene, toluene, chloroform or benzene
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| 6. FIXATIVE CAN INCLUDE: | Helly's Zenker's and Boulin's
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| 7.WHEN DEALING WITH PICRIC ACID: | Always keep the picric acid wet
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| 8. A TEST FOR DECALCIFICATION COULD BE: | K ion determinations
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| 9. THE MOST COMMON FORM OF EMBEDDING MEDIA IS: | Paraffin wax
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| 10. WHEN DECALCIFYING BONE THE VOLUME OF FLUID USED PER VOLUME OF TISSUE IS: | 20x
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| 11. PUTREFACTION IS THE SAME AS: | Bacterial decomposition
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| 12. MOST ROUTINE MICROTOMY IS PERFORMED ON THIS TYPE OF MICROTOME | Rotary
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| 13. THE ANGLE OF CLEARANCE IS: | The angle made by the block face and the bevel of the knife
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| 14. TISSUE SECTIONS WITH A CALCIFIED SURFACE SHOULD BE PLACED SO THAT: | The knife passes over this part last
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| 15. WHEN EMBEDDING MULTIPLE TISSUE SAMPLES IN ONE MOLD: | The pieces of tissue should touch each other
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| 16. MOST FIXATIVE ARE USED AT: | Room temperature
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| 17. ADVANTAGES OF FORMALIN FIXATION INCLUDE: | No excessive hardening, Inexpensive, Good penetration
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| 18. DISADVANTAGES OF FORMALINE FIXATION INCLUDE: | Irritating odor,Reduction in PAS positively
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| 19. TO PREVENT ACID FORMALDEHYDE HAEMATEIN: | Use buffered formalin
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| 20. TO REMOVE MERCURY PIGMENT: | Use iodine and sodium thiosulfate alcoholic iodine
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| 21. PICRATE ARTIFACTS ARE not DUE TO: | Chromium fixative being present, precipitation of mercuric chloride, production of picrate dichloride
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| 22. LARGE PIECES OF BRAIN: | Are often embedded in cellulose nitrate
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| 23. A DISADVANTAGE OF PARAFFIN WAX EMBEDDING IS: | Inabilit to cut very thin sections
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| 24. FIXATION: | Alters the way tissues bend light, Is an artifact, Prevents autolysis
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| 25. A STABILIZER FOR A FORMALIN FIXATIVE IS: | Methanol
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| 26. A 5% SOLUTION OF FORMALIN does not CONTAIN: | 2%, 4%, 5%, 10%, formaldehyde
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| 27. FORMALIN | Preserves lipids well
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| 28. PINK DISEASE IS SEEN IN: | Formalin fixed tissue wiwth H&E stain
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| 29. ZENKER'S FLUID CONTAINS: | Mercuric chloride and acetic acid
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| 30. CARNOY'S FLUID CAUSES: | Shrinkage
Lysis of red cells
Preservation of glycogen
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| 31. THE APPROPRIATE FIXATIVE FOR BONE MARROW PREPS AND BLOOD SMEARS IS: | Alcohol
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| 32. THE MORE DELICATE A TISSUE IS THE: | Lower the % of alcohol it needs
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| 33. THE MELTING POINT OF PARAFFIN WAX: | Is higher as the wax gets harder
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| 34. VACUME IMPREGNATION: | Is not required for tissue such as spleen, Is not recommended for cysts. Does not require a longer fixation time.
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| 35. TISSUE IMBEDDED IN CELLULOSE NITRATE: | Requires storage in alcohol
Will not ribbon
Are usually very hard
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| 36. DECALCIFICATION: | Is best done in a 25 degrees celcius water bath
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| 37. THE APPROPRIATE VOLUME OF EDTA IS_____OF THE TISSUE VOLUME: | 150%
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| 38. TRICHLOROACETIC ACID SHOULD BE USED IN A CONCENTRATION OF ___% WHILE FORMIC ACID SHOULD BE AT ___% | 5/10
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| 39. THE FIRST STEP IN SHARPENING A KNIFE IS )___ AND THE KNIFE EDGE COMES ____ | honing / first
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| 40. TO ADHERE THE TISSUE SECTION TO THE SLIDE ____ | Albumin
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| 41. IN AN H & E STAIN: | The nuclei are blue
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| 42. DRYING TISSUE SECTION SHOULD BE AT ___FOR ___HOURS | 37 degree celcius / 16
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| 43. THE MOST COMMON MORDANT FOR H & E IS: | Na metabisulphite
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| 44. MORDANTS ARE: | Necessary for tissue staining
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| 45.THE DECOLORIZER FOR H & E IS ___AND IT WORKS BY ___ | Acid-alcohol / breaking the lake
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| 46. ARTIFICIAL RIPENING AGENTS INCLUDE: | Na iodate and K permanganate
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| 47. TAKING SECTIONS TO WATER REQUIRES: | Clearing and rehydrating
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