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Histology

HISTOLOGY

QuestionAnswer
1. THE PURPOSE OF A FIXATIVE IS TO Harden tissue for further processing
2. THE TERM DENATURE APPLIES TO Protein
3. A "PLANOCONCAVE" MICROTOME KNIFE MAY BE DEFINED AS: Having one side concave and one side straight
4. THE PROPER ORDER OF TISSUE PROCESSING IS: Fixation, decalcification, dehydration, clearing and embedding
5. The clearing stage of tissue processing: Is an intermediate step between alcohols and paraffin. Is an intermediate step between dehydrating reagents and embedding media Can be dne with exylene, toluene, chloroform or benzene
6. FIXATIVE CAN INCLUDE: Helly's Zenker's and Boulin's
7.WHEN DEALING WITH PICRIC ACID: Always keep the picric acid wet
8. A TEST FOR DECALCIFICATION COULD BE: K ion determinations
9. THE MOST COMMON FORM OF EMBEDDING MEDIA IS: Paraffin wax
10. WHEN DECALCIFYING BONE THE VOLUME OF FLUID USED PER VOLUME OF TISSUE IS: 20x
11. PUTREFACTION IS THE SAME AS: Bacterial decomposition
12. MOST ROUTINE MICROTOMY IS PERFORMED ON THIS TYPE OF MICROTOME Rotary
13. THE ANGLE OF CLEARANCE IS: The angle made by the block face and the bevel of the knife
14. TISSUE SECTIONS WITH A CALCIFIED SURFACE SHOULD BE PLACED SO THAT: The knife passes over this part last
15. WHEN EMBEDDING MULTIPLE TISSUE SAMPLES IN ONE MOLD: The pieces of tissue should touch each other
16. MOST FIXATIVE ARE USED AT: Room temperature
17. ADVANTAGES OF FORMALIN FIXATION INCLUDE: No excessive hardening, Inexpensive, Good penetration
18. DISADVANTAGES OF FORMALINE FIXATION INCLUDE: Irritating odor,Reduction in PAS positively
19. TO PREVENT ACID FORMALDEHYDE HAEMATEIN: Use buffered formalin
20. TO REMOVE MERCURY PIGMENT: Use iodine and sodium thiosulfate alcoholic iodine
21. PICRATE ARTIFACTS ARE not DUE TO: Chromium fixative being present, precipitation of mercuric chloride, production of picrate dichloride
22. LARGE PIECES OF BRAIN: Are often embedded in cellulose nitrate
23. A DISADVANTAGE OF PARAFFIN WAX EMBEDDING IS: Inabilit to cut very thin sections
24. FIXATION: Alters the way tissues bend light, Is an artifact, Prevents autolysis
25. A STABILIZER FOR A FORMALIN FIXATIVE IS: Methanol
26. A 5% SOLUTION OF FORMALIN does not CONTAIN: 2%, 4%, 5%, 10%, formaldehyde
27. FORMALIN Preserves lipids well
28. PINK DISEASE IS SEEN IN: Formalin fixed tissue wiwth H&E stain
29. ZENKER'S FLUID CONTAINS: Mercuric chloride and acetic acid
30. CARNOY'S FLUID CAUSES: Shrinkage Lysis of red cells Preservation of glycogen
31. THE APPROPRIATE FIXATIVE FOR BONE MARROW PREPS AND BLOOD SMEARS IS: Alcohol
32. THE MORE DELICATE A TISSUE IS THE: Lower the % of alcohol it needs
33. THE MELTING POINT OF PARAFFIN WAX: Is higher as the wax gets harder
34. VACUME IMPREGNATION: Is not required for tissue such as spleen, Is not recommended for cysts. Does not require a longer fixation time.
35. TISSUE IMBEDDED IN CELLULOSE NITRATE: Requires storage in alcohol Will not ribbon Are usually very hard
36. DECALCIFICATION: Is best done in a 25 degrees celcius water bath
37. THE APPROPRIATE VOLUME OF EDTA IS_____OF THE TISSUE VOLUME: 150%
38. TRICHLOROACETIC ACID SHOULD BE USED IN A CONCENTRATION OF ___% WHILE FORMIC ACID SHOULD BE AT ___% 5/10
39. THE FIRST STEP IN SHARPENING A KNIFE IS )___ AND THE KNIFE EDGE COMES ____ honing / first
40. TO ADHERE THE TISSUE SECTION TO THE SLIDE ____ Albumin
41. IN AN H & E STAIN: The nuclei are blue
42. DRYING TISSUE SECTION SHOULD BE AT ___FOR ___HOURS 37 degree celcius / 16
43. THE MOST COMMON MORDANT FOR H & E IS: Na metabisulphite
44. MORDANTS ARE: Necessary for tissue staining
45.THE DECOLORIZER FOR H & E IS ___AND IT WORKS BY ___ Acid-alcohol / breaking the lake
46. ARTIFICIAL RIPENING AGENTS INCLUDE: Na iodate and K permanganate
47. TAKING SECTIONS TO WATER REQUIRES: Clearing and rehydrating
Created by: nugentbarbara
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