Busy. Please wait.
or

show password
Forgot Password?

Don't have an account?  Sign up 
or

Username is available taken
show password

why


Make sure to remember your password. If you forget it there is no way for StudyStack to send you a reset link. You would need to create a new account.
We do not share your email address with others. It is only used to allow you to reset your password. For details read our Privacy Policy and Terms of Service.


Already a StudyStack user? Log In

Reset Password
Enter the associated with your account, and we'll email you a link to reset your password.

Remove Ads
Don't know
Know
remaining cards
Save
0:01
To flip the current card, click it or press the Spacebar key.  To move the current card to one of the three colored boxes, click on the box.  You may also press the UP ARROW key to move the card to the "Know" box, the DOWN ARROW key to move the card to the "Don't know" box, or the RIGHT ARROW key to move the card to the Remaining box.  You may also click on the card displayed in any of the three boxes to bring that card back to the center.

Pass complete!

"Know" box contains:
Time elapsed:
Retries:
restart all cards




share
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.

  Normal Size     Small Size show me how

Histology

HISTOLOGY

QuestionAnswer
1. THE PURPOSE OF A FIXATIVE IS TO Harden tissue for further processing
2. THE TERM DENATURE APPLIES TO Protein
3. A "PLANOCONCAVE" MICROTOME KNIFE MAY BE DEFINED AS: Having one side concave and one side straight
4. THE PROPER ORDER OF TISSUE PROCESSING IS: Fixation, decalcification, dehydration, clearing and embedding
5. The clearing stage of tissue processing: Is an intermediate step between alcohols and paraffin. Is an intermediate step between dehydrating reagents and embedding media Can be dne with exylene, toluene, chloroform or benzene
6. FIXATIVE CAN INCLUDE: Helly's Zenker's and Boulin's
7.WHEN DEALING WITH PICRIC ACID: Always keep the picric acid wet
8. A TEST FOR DECALCIFICATION COULD BE: K ion determinations
9. THE MOST COMMON FORM OF EMBEDDING MEDIA IS: Paraffin wax
10. WHEN DECALCIFYING BONE THE VOLUME OF FLUID USED PER VOLUME OF TISSUE IS: 20x
11. PUTREFACTION IS THE SAME AS: Bacterial decomposition
12. MOST ROUTINE MICROTOMY IS PERFORMED ON THIS TYPE OF MICROTOME Rotary
13. THE ANGLE OF CLEARANCE IS: The angle made by the block face and the bevel of the knife
14. TISSUE SECTIONS WITH A CALCIFIED SURFACE SHOULD BE PLACED SO THAT: The knife passes over this part last
15. WHEN EMBEDDING MULTIPLE TISSUE SAMPLES IN ONE MOLD: The pieces of tissue should touch each other
16. MOST FIXATIVE ARE USED AT: Room temperature
17. ADVANTAGES OF FORMALIN FIXATION INCLUDE: No excessive hardening, Inexpensive, Good penetration
18. DISADVANTAGES OF FORMALINE FIXATION INCLUDE: Irritating odor,Reduction in PAS positively
19. TO PREVENT ACID FORMALDEHYDE HAEMATEIN: Use buffered formalin
20. TO REMOVE MERCURY PIGMENT: Use iodine and sodium thiosulfate alcoholic iodine
21. PICRATE ARTIFACTS ARE not DUE TO: Chromium fixative being present, precipitation of mercuric chloride, production of picrate dichloride
22. LARGE PIECES OF BRAIN: Are often embedded in cellulose nitrate
23. A DISADVANTAGE OF PARAFFIN WAX EMBEDDING IS: Inabilit to cut very thin sections
24. FIXATION: Alters the way tissues bend light, Is an artifact, Prevents autolysis
25. A STABILIZER FOR A FORMALIN FIXATIVE IS: Methanol
26. A 5% SOLUTION OF FORMALIN does not CONTAIN: 2%, 4%, 5%, 10%, formaldehyde
27. FORMALIN Preserves lipids well
28. PINK DISEASE IS SEEN IN: Formalin fixed tissue wiwth H&E stain
29. ZENKER'S FLUID CONTAINS: Mercuric chloride and acetic acid
30. CARNOY'S FLUID CAUSES: Shrinkage Lysis of red cells Preservation of glycogen
31. THE APPROPRIATE FIXATIVE FOR BONE MARROW PREPS AND BLOOD SMEARS IS: Alcohol
32. THE MORE DELICATE A TISSUE IS THE: Lower the % of alcohol it needs
33. THE MELTING POINT OF PARAFFIN WAX: Is higher as the wax gets harder
34. VACUME IMPREGNATION: Is not required for tissue such as spleen, Is not recommended for cysts. Does not require a longer fixation time.
35. TISSUE IMBEDDED IN CELLULOSE NITRATE: Requires storage in alcohol Will not ribbon Are usually very hard
36. DECALCIFICATION: Is best done in a 25 degrees celcius water bath
37. THE APPROPRIATE VOLUME OF EDTA IS_____OF THE TISSUE VOLUME: 150%
38. TRICHLOROACETIC ACID SHOULD BE USED IN A CONCENTRATION OF ___% WHILE FORMIC ACID SHOULD BE AT ___% 5/10
39. THE FIRST STEP IN SHARPENING A KNIFE IS )___ AND THE KNIFE EDGE COMES ____ honing / first
40. TO ADHERE THE TISSUE SECTION TO THE SLIDE ____ Albumin
41. IN AN H & E STAIN: The nuclei are blue
42. DRYING TISSUE SECTION SHOULD BE AT ___FOR ___HOURS 37 degree celcius / 16
43. THE MOST COMMON MORDANT FOR H & E IS: Na metabisulphite
44. MORDANTS ARE: Necessary for tissue staining
45.THE DECOLORIZER FOR H & E IS ___AND IT WORKS BY ___ Acid-alcohol / breaking the lake
46. ARTIFICIAL RIPENING AGENTS INCLUDE: Na iodate and K permanganate
47. TAKING SECTIONS TO WATER REQUIRES: Clearing and rehydrating
Created by: nugentbarbara