Heme Cytochemical Stains and Markers
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| PAS | Periodic Acid Schiff, indicated Glycogen, seen in Erythrolukemia; ALL
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| Prussian Blue | Indicates Iron, see in sideroblastic anemia
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| LAP | Indicates Alkaline Phosphatase; increased in Leukemoid Reaction and P.Vera, decreased in CML
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| Peroxidase/SBB | Indicates Myeloperoxidase and Lipids; AML M1 neg, M4 pos, AML M5 neg, ALL neg, neutrophils stain intensly, selectively identifies primary and secondary granueles of myeloid cells
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| Specific Esterase | Indicates esterase in granulocyte percursors, AML M1 neg, M4 pos, AML M5 neg, uses chloracetate as substrate
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| Non-specific Esterase | Indicates non specific esterase in Monocyte precursors, AML M4 pos, AML M5 pos
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| TRAP | Indicates tartrate-resistant acid phosphatase, pos in hairy cell leukemia
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| Auer Rods | Indicate coalition of primary granules, AML pos, ALL neg
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| CD 13, CD 33 | Indicates Myeloblasts, AML pos, ALL neg
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| CD 2, CD 3, CD 5, CD 7 | Indicates T-cell lineage, T-ALL pos, B-ALL neg, AML neg
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| CD 10, CD 19, CD 22 | Indicates B-cell lineage, B-ALL pos, T-ALL neg, AML-neg
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| t (15; 17) | translocation involving retinoic acid receptor gene; AML-M3 pos
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| CD 34 | indicates stem cells
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| CD 42, CD 61 | Indicates megakaryocytes; seen in AML M7
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| CD 116, CD 14 | Indicates monoblasts; seen in AML M4 and AML M5
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| t (9; 22) | CML; philadelphia chromosome
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