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Stains/Markers
Heme Cytochemical Stains and Markers
Question | Answer |
---|---|
PAS | Periodic Acid Schiff, indicated Glycogen, seen in Erythrolukemia; ALL |
Prussian Blue | Indicates Iron, see in sideroblastic anemia |
LAP | Indicates Alkaline Phosphatase; increased in Leukemoid Reaction and P.Vera, decreased in CML |
Peroxidase/SBB | Indicates Myeloperoxidase and Lipids; AML M1 neg, M4 pos, AML M5 neg, ALL neg, neutrophils stain intensly, selectively identifies primary and secondary granueles of myeloid cells |
Specific Esterase | Indicates esterase in granulocyte percursors, AML M1 neg, M4 pos, AML M5 neg, uses chloracetate as substrate |
Non-specific Esterase | Indicates non specific esterase in Monocyte precursors, AML M4 pos, AML M5 pos |
TRAP | Indicates tartrate-resistant acid phosphatase, pos in hairy cell leukemia |
Auer Rods | Indicate coalition of primary granules, AML pos, ALL neg |
CD 13, CD 33 | Indicates Myeloblasts, AML pos, ALL neg |
CD 2, CD 3, CD 5, CD 7 | Indicates T-cell lineage, T-ALL pos, B-ALL neg, AML neg |
CD 10, CD 19, CD 22 | Indicates B-cell lineage, B-ALL pos, T-ALL neg, AML-neg |
t (15; 17) | translocation involving retinoic acid receptor gene; AML-M3 pos |
CD 34 | indicates stem cells |
CD 42, CD 61 | Indicates megakaryocytes; seen in AML M7 |
CD 116, CD 14 | Indicates monoblasts; seen in AML M4 and AML M5 |
t (9; 22) | CML; philadelphia chromosome |