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Stains/Markers

Heme Cytochemical Stains and Markers

QuestionAnswer
PAS Periodic Acid Schiff, indicated Glycogen, seen in Erythrolukemia; ALL
Prussian Blue Indicates Iron, see in sideroblastic anemia
LAP Indicates Alkaline Phosphatase; increased in Leukemoid Reaction and P.Vera, decreased in CML
Peroxidase/SBB Indicates Myeloperoxidase and Lipids; AML M1 neg, M4 pos, AML M5 neg, ALL neg, neutrophils stain intensly, selectively identifies primary and secondary granueles of myeloid cells
Specific Esterase Indicates esterase in granulocyte percursors, AML M1 neg, M4 pos, AML M5 neg, uses chloracetate as substrate
Non-specific Esterase Indicates non specific esterase in Monocyte precursors, AML M4 pos, AML M5 pos
TRAP Indicates tartrate-resistant acid phosphatase, pos in hairy cell leukemia
Auer Rods Indicate coalition of primary granules, AML pos, ALL neg
CD 13, CD 33 Indicates Myeloblasts, AML pos, ALL neg
CD 2, CD 3, CD 5, CD 7 Indicates T-cell lineage, T-ALL pos, B-ALL neg, AML neg
CD 10, CD 19, CD 22 Indicates B-cell lineage, B-ALL pos, T-ALL neg, AML-neg
t (15; 17) translocation involving retinoic acid receptor gene; AML-M3 pos
CD 34 indicates stem cells
CD 42, CD 61 Indicates megakaryocytes; seen in AML M7
CD 116, CD 14 Indicates monoblasts; seen in AML M4 and AML M5
t (9; 22) CML; philadelphia chromosome
Created by: mnuesmeyer