Intro to Microbiology, Staphylococci, Streptococci, Gram Positive Bacilli
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| Bacitracin = S Furazolidone = R Modified oxidase = Blue Catalase = Bubbles These test results identify the MO as: | show 🗑
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| Motility = negative at 35 & positive at 25 Catalase = positive Hemolysis = beta Bile-esculin = Black Gram stain = Small, Gram-positive coccobacillary What is the identification? | show 🗑
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| show | GN & Selenite = isolation Salmonella & Shigella
LIM & Trans-Vag = isolation GBS
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| What is the purpose of the Quellung reaction? | show 🗑
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| What reagent is used for the PYR test? | show 🗑
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| show | No; many types of normal flora streptococcus (not GAS) are present
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| What is the primary disease caused by Streptococcus pneumoniae? | show 🗑
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| show | Endotoxin; found in lipopolysaccharide part of cell wall
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| Why is methylene blue stain used for detection of Corynebacterium diphtheriae? | show 🗑
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| What type of hemolysis is exhibited by Corynebacterium species? | show 🗑
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| What type of hemolysis is considered no hemolysis (nonhemolytic)? | show 🗑
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| What test is performed only on Staphylococcus coagulase-negative isolates from urine cultures? | show 🗑
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| An anaerobic atmosphere is without __________ and an example of an anaerobic organism is ______________. | show 🗑
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| What type of media are MAC & EMB? What is the purpose of using the media? | show 🗑
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| What is another name for Rothia mucilaginosus (Stomatococcus)? | show 🗑
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| show | 1/10 dilution of bleach (NaClO); clean 1st (soap & water) & then disinfect
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| Agar media melts at _________ and stays liquid until 45-50o C (or solidifies at 45-50o C). | show 🗑
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| show | 15 psi for 121 C degrees for 15 minutes
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| show | 35 to 37 degrees C (body temperature)
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| What are the three most common temperatures used in the laboratory to isolate bacteria? | show 🗑
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| show | 7% Ferric chloride
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| What the genus names for satelliting strep? | show 🗑
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| An animal infectious disease transmitted to humans is referred to as zoonosis. Which zoonotic organisms have we studied? | show 🗑
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| show | Bacillus anthracis
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| show | Bacillus cereus and Listeria
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| Name an aerobic, spore-forming genus | show 🗑
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| show | Bacillus species
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| Motility = negative Catalase = positive Hemolysis = usually gamma Gram stain = "Chinese letters" What is the identification? | show 🗑
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| show | Enterococcus species
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| show | Enterococcus & satelliting streptococcus
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| show | Enterococcus species
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| show | Erysipelothrix
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| show | Erysipelothrix
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| show | Lactobacillus
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| show | Listeria monocytogenes
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| show | Listeria monocytogenes
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| Which streptococcus-like bacteria are vancomycin resistant? | show 🗑
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| show | Rothia mucilaginosus (Stomatococcus)
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| Tube coagulase positive What is the identification ? | show 🗑
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| Slide coagulase result: saline = no clumps plasma = clumps What is the identification? | show 🗑
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| show | Streptococcus agalactiae
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| Bile-esculin = No color change 6.5% salt = No growth P disk = S What is the identification ? | show 🗑
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| show | Streptococcus pneumoniae
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| A disk = S CAMP = negative PYR = positive SXT = R What is the identification ? | show 🗑
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| A physician sends a throat swab to the laboratory, requesting antigen testing for Group A streptococcus. What are the correct responses to positive antigen test results and negative test results? | show 🗑
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| show | A & SXT disks; PYR; CAMP or hippurate hydrolysis & Serogrouping
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| show | Aerobically & anaerobically
Staphylococcus & Streptococcus
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| What type of hemolysis has a zone of greenish discoloration? | show 🗑
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| show | Alpha and Gamma
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| What type of hemolysis is demonstrated by Streptococcus pneumoniae, viridans streptococcus, Enterococcus species, & nonenterococcal Group D streptococcus? | show 🗑
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| show | Bacillus anthracis
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| What reagent is in the A disk? | show 🗑
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| What media are used to isolate Corynebacterium diphtheriae? | show 🗑
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| What type of hemolysis involves the complete lysis of RBCs? | show 🗑
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| What type of hemolysis is demonstrated by Groups A, B, C, F, & G Streptococcus? | show 🗑
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| What type of hemolysis is exhibited by Arcanobacterium haemolyticum? | show 🗑
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| What type of hemolysis is exhibited by Listeria monocytogenes? | show 🗑
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| show | Beta hemolytic, + for hippurate & CAMP & cause meningitis/bacteremia in babies
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| Which organism is used to perform the CAMP test? | show 🗑
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| show | Bile salts (sodium deoxycholate)
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| Name two examples of nonselective media. | show 🗑
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| show | Blue-purple
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| Slide coagulase test detects __________________ . | show 🗑
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| The staphylococcus latex aggulutination test detects ________ & _________. In addition some tests detect capsular polysaccharides. | show 🗑
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| How are CO2 levels checked in CO2 incubators? | show 🗑
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| What tests are appropriate for gamma-hemolytic, catalase negative, GPC organisms? | show 🗑
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| show | Chocolate agar, selective media for pathogenic Neisseria, & Campylobacter agar
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| Capnophilic organisms require an increased amount of _____________ and an example of a capnophilic organism is ___________ _____________. | show 🗑
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| Which organisms are referred to as having diphtheroid or coryneform morphology? | show 🗑
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| List the Gram stain reagents in the correct order for test performance. | show 🗑
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| show | Daily
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| Which test is used to determines C. diphtheriae toxin formers? | show 🗑
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| show | Exotoxin
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| Tube coagulase test detects __________________ . | show 🗑
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| show | Gamma hemolysis
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| show | Gamma hemolysis
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| show | Group A Streptococcus
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| show | Gram-positive cocci in clumps (clusters)
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| show | Gram-positive cocci in pairs (lancet shaped)
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| show | Gram-positive cocci in tetreds
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| show | Gram-positive, small pleomorphic rods ("Chinese" letters)
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| show | Gram-positive, small rods (may be coccobacillary)
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| show | Group A Streptococcus
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| A disk = R Hippurate = Positive PYR = Negative SXT = R What is the identification ? | show 🗑
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| What reagent is used for the catalase test? | show 🗑
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| Humidophilic organisms require increased ___________ and an example of a humidophilic organism is _____________ _____________ | show 🗑
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| show | MAC, EMB, CNA, PEA, & BAP
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| What reagent is used for the hippurate test if glycine is detected? | show 🗑
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| Bile-esculin = Positive 6.5% salt = No growth Optochin = R What is the identification ? | show 🗑
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| show | Nutrient agar & trypticase soy broth
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| What reagent is in the P disk? | show 🗑
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| Microaerobic organisms require a decreased amount of ___________ and an example of a microaerobic organism is _______________. | show 🗑
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| What tests are appropriate for alpha-hemolytic, catalase negative, GPC organisms? | show 🗑
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| Slide coagulase results: saline = clumps plasma = clumps What should be the next step? | show 🗑
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| show | Pink to red
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| show | Plasma
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| Slide coagulase negative What is the identification? | show 🗑
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| show | pyridoxal (vitamin B6)
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| Appearance of Staphylococcus coagulase negative & Micrococcus on MSA agar (MOs don't ferment mannitol) | show 🗑
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| show | Satelliting streptococci
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| show | Selective media; used to isolate GP organisms (most GNs are inhibited)
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| PYR = Red Hippurate = Purple What is the identification ? | show 🗑
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| Which organism is known to cause an aggressive form of endocarditis? Which specimens would the organisms most likely be detected in? | show 🗑
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| What reagents are used for the modified oxidase test? | show 🗑
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| show | The autoclaved materials were not sterilized; the process has to be repeated.
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| Bile-esculin = No color change 6.5% salt = Purple P disk = R What is the identification ? | show 🗑
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| Appearance of Staphylococcus aureus on mannitol salt agar - ferments mannitol | show 🗑
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Created by:
Mwortman
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