Lab
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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|---|---|---|---|---|---|
| show | Too little/much blade tilt,
poorly adjusted chuck,
cutting too fast or at inconsistent speeds,
old or out of adjustment microtome,
loose screws/parts
🗑
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| Crooked ribbons may be caused by: | show 🗑
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| Sections sticking to knife may be caused by: | show 🗑
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| Smeared/deformed sections may be caused by: | show 🗑
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| show | Block not clamped tightly,
too much knife tilt,
loose screws/parts,
old microtome,
under/overfilled block not sitting properly,
often happens w/ hard tissues (e.g. uterus)
🗑
|
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| show | Cold room - warm knife slightly,
too much knife angle,
too thick sections,
dull knife
🗑
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| show | Dull knife,
paraffin build-up on blade,
too little knife tilt,
cutting too fast,
warm room,
warm block
🗑
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| Knife rings on upstroke & sections are scratched | show 🗑
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| show | Too little knife angle,
dull knife,
warm room - cool block,
soft paraffin
🗑
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| show | Knife angle is too severe
🗑
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| Scratches or splits in ribbon may be caused by: | show 🗑
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| Fragmented or torn sections may be caused by: | show 🗑
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| Sections flying due to static may be helped by: | show 🗑
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| show | Dry, over-processed tissue,
too much knife tilt,
cutting too fast,
block holder shaft over-extended - vibration
🗑
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| show | Facing block too roughly, tears tissue,
can try to face further & smooth
🗑
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| Parched earth artifact caused by: | show 🗑
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| show | Not laying ribbon on bath carefully,
bath not warm enough
🗑
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| show | Wearing gloves,
not sticking fingers in bath
🗑
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| Prevent cross-contamination by: | show 🗑
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| To help keep sections on slide may use: | show 🗑
|
Review the information in the table. When you are ready to quiz yourself you can hide individual columns or the entire table. Then you can click on the empty cells to reveal the answer. Try to recall what will be displayed before clicking the empty cell.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
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Created by:
CCF
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