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Ch 7: Microbial Genetics

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Term
Definition
Ames test   Ames test  
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Anticodon   Portion of tRNA that is complementary to a codon on mRNA  
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Auxotrophs   A mutant organism that requires a particular additional nutrient which the wild-type strain does not.  
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Base-excision repair   A method of DNA repair where an enzyme system removes the incorrect base and fills in the gap  
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Carcinogens   Cancer-causing agents  
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Chromosome   A circular molecule of DNA associated with protein and RNA molecules  
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Codon   Triplets of mRNA nucleotides that code for specific amino acids  
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Conjugation   Plasmid genes are transferred from a living donor cell to a recipient cell via direct contact  
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Genes   Specific sequences of nucleotides that code for RNA or polypeptide molecules  
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Genetics   The study of inheritance and inheritable traits  
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Genome   The sum of all the genetic material in a cell or virus  
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Genotype   Actual set of genes in an organisms genome  
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Horizontal gene transfer   General term where DNA from a donor cell is transmitted to a recipient cell  
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Inducible operons   Type of operon that is not normally transcribed until activated by inducers  
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Lagging strand   New DNA strand that is discontinuously synthesized in a direction away from the replication fork in series of short fragments  
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Leading strand   New DNA strand that is synthesized continuously toward the replication fork.  
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Missense mutation   A mutation in which what gets transcribed and translated makes sense, but not the right sense  
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Mutagen   Physical or chemical agent that causes a mutation in DNA  
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Mutation   A heritable change in the nucleotide sequence of a genome  
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Nonsense mutation   A base-pair substitution changes an amino acid codon into a stop codon  
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Nucleotide analogs   Compounds that are structurally similar to normal nucleotides but, when incorporated into DNA, may interfere with DNA polymerase function or cause base-pair mismatching  
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Operon   A series of genes controlled by one regulatory gene  
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Phenotype   The physical and functional traits expressed by an organism's genes  
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Plasmids   Extrachromosomal DNA molecules  
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Point mutations   Mutations in which one nucleotide base pair is affected  
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Pyrimidine dimers   Adjacent pyrimidine bases to bond to one another  
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Quorum sensing   Cells detect molecules and synthesize new proteins in response to the signals  
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Recombination   Refers to the exchange of nucleotide sequences between two DNA molecules  
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Repressible operons   Type of operon that is continually transcribed until deactivated by repressors  
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Revertants   Cells that have regained wild-type phenotype  
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RNA polymerase   An enzyme that binds to a specific region of DNA called a promoter  
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RNA primer   Molecules for DNA polymerase to use during DNA replication.  
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Silent mutations   A mutation where the substitution does not change the amino acid sequence  
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Transcription   Process that produces a complementary RNA copy of the gene  
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Transduction   A bacteriophage carries DNA from a donor cell to a recipient cell  
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Transformation   A recipient cell takes up DNA from the environment  
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Translation   The process used to synthesize polypeptides  
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Transposons   DNA segments that contains palindromic sequences known as inverted repeats (IR) at each end  
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Vertical gene transfer   Transmission of genes from parents to offspring  
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Wild-type   Cells normally found in nature are called __________  
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