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Spirochetes

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Answer
What special stains are used for B. recurrentis, B. burgdorferi, and L. interrogans?   B. recurrentis: Giemsa, Wrights, or Acridine Orange B. bergdorferi: Warthin-Starry silver stain and Acridine Orange L. interrogans: silver impregnation  
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What species causes "relapsing fever" and what transmits the disease?   Borrelia recurrentis louse or ticks  
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Borrelia burgdorferi causes what disease and how would one know they have it?   Lyme disease In the first week they would have a bulls-eye around the deer tick bite  
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What is a quick way to differentiate which bacteria caused rate bite fever?   Do a gram stain! Spirillum minor is a spiral GNR & Streptobacillus moniliforms is a normal GNR  
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What are the media and growth requirements for Leptospira interrogans?   plated on Fletcher's Stuart's media at 30C for 6-14 days up to 6 weeks  
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What can also be used to identify Spirochetes since they do not stain well?   DFA (direct immunofluorescence)  
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What are the media and growth requirements for Borrelia burgdorferi?   plated on Kelly's media at 33C for 1 month  
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