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Unit 7

Spirochetes

QuestionAnswer
What special stains are used for B. recurrentis, B. burgdorferi, and L. interrogans? B. recurrentis: Giemsa, Wrights, or Acridine Orange B. bergdorferi: Warthin-Starry silver stain and Acridine Orange L. interrogans: silver impregnation
What species causes "relapsing fever" and what transmits the disease? Borrelia recurrentis louse or ticks
Borrelia burgdorferi causes what disease and how would one know they have it? Lyme disease In the first week they would have a bulls-eye around the deer tick bite
What is a quick way to differentiate which bacteria caused rate bite fever? Do a gram stain! Spirillum minor is a spiral GNR & Streptobacillus moniliforms is a normal GNR
What are the media and growth requirements for Leptospira interrogans? plated on Fletcher's Stuart's media at 30C for 6-14 days up to 6 weeks
What can also be used to identify Spirochetes since they do not stain well? DFA (direct immunofluorescence)
What are the media and growth requirements for Borrelia burgdorferi? plated on Kelly's media at 33C for 1 month
Created by: martha.elder