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|What special stains are used for B. recurrentis, B. burgdorferi, and L. interrogans?
|B. recurrentis: Giemsa, Wrights, or Acridine Orange B. bergdorferi: Warthin-Starry silver stain and Acridine Orange L. interrogans: silver impregnation
|What species causes "relapsing fever" and what transmits the disease?
|Borrelia recurrentis louse or ticks
|Borrelia burgdorferi causes what disease and how would one know they have it?
|Lyme disease In the first week they would have a bulls-eye around the deer tick bite
|What is a quick way to differentiate which bacteria caused rate bite fever?
|Do a gram stain! Spirillum minor is a spiral GNR & Streptobacillus moniliforms is a normal GNR
|What are the media and growth requirements for Leptospira interrogans?
|plated on Fletcher's Stuart's media at 30C for 6-14 days up to 6 weeks
|What can also be used to identify Spirochetes since they do not stain well?
|DFA (direct immunofluorescence)
|What are the media and growth requirements for Borrelia burgdorferi?
|plated on Kelly's media at 33C for 1 month