Media selection
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| 5% Sheep Blood Agar is non-selective but differential for what? | Differentiates alpha, beta and nonhemolytic bacteria.
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| Blood Agar is composed of what nutrient? | 5% sheep blood
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| What types of organisms are supported by the blood agar? | All but the most fastidious organisms. Most gram pos and gram neg bacteria
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| What is Brilliant Green differential for? | Lactose pos and Lactose neg bacteria.
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| Lactose pos on Brilliant Green decreases pH and is what color? | yellow green
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| on Brilliant Green what colors will be seen on Lactose neg? | pink colony with red zone in medium
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| What type of bacteria will Brilliant Green inhibit? | most enteric bacteria
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| What kind of agar is the Chocolate Agar? | Enriched
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| How is a Chocolate Agar enriched? | It is a Blood agar base with hemoglobin, hemin and co enzyme nicotine adenine dinucleotide(NAD)
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| what factor is hemin in a chocolate agar? | V Factor
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| What factor is NAD in a chocolate agar? | X Factor
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| The chocolate auger is enriched with X factor and V factor to support the growth of what? | H. influenzae and N. gonorrhaeae, plus most other gram pos and gram neg bacteria.
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| How is Columbia CNA agar differential? | it will demonstrate alpha /beta/no hemolysis
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| Columbia CNA agar is selective for what? | Gram pos bacteria.
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| The Columbia CNA agar has the ability to grow what? | Staph and Strep.
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| On the Columbia CNA agar what inhibits gram neg bacteria from growing? | Colistan and nalidix acid
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| The EMB ager contains what two dyes? | Eosin Y and Methylene Blue
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| The dyes in the EMB agar cause it to be selective by what? | The dyes inhibit gram pos rods.
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| The EMB agar is differential how? | Gram neg lactose fermentation
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| On the EMB agar what color will a lactose pos be. | Dark purple center, Lactose neg will not have a dark purple center.
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| Hektoen Enteric Agar inhibits wh |
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| Hektoen Enteric agar is differential for what? | Lactose pos and neg bacteria
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| On the HE agar how do you differentiate between lactose pos and neg? | decreased pH will cause yellow colonies for L+ and green blue for L-.
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| What type of bacteria will the HE agar inhibit? | most normal enteric bacteria.
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| Selenite F broth is used to enrich what bacteria | Salmonella and other patogenic enteric bacteria
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| In Selenite F broth what is used to inhibit normal flora? | sodium selenite
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| The Thayer Martin Agar is selective for what? | N. gonorrrhoeae and some other Neisseria species.
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| What is the TM agar made of | colistan to inhibit other gram neg
Vancomycin to inhibit gram pos
nystatin to inhibit yeast
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| Modified TM contains what? | trimethoprim to inhibit Proteus.
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| Thioglycollate Broth is used for what? | enrichment for most all bacteria
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| Mac Conkey is selective for what? | enteric gram neg
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| Mac Conkey differentiate Lactose fermenters how? | Lactose + is red while Lactose- is clear
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| in the Mac Conkey agar what causes inhibition of gram + bacteria? | crystal violet dye and protein.
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| What species is Mannitol salt selective for? | Staph
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| In Mannitol Salt Agar what is used to inhibit gram- and other gram pos bacteria besides staph? | NaCl
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| Mannitol fermenters and be differentiated from non fermenters be? | fermenters are yellow and non-fermenters are pink.
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| PEA agar is used for? | same as CNA
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| What does SXT stand for? | Strep Selective Agar.
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| What kind of culture is most likely used with a SXT agar? | throat cultures
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| What is the SXT agar composed of? | Sheep blood agar with trimethoprim-sulfa an antibiotic used to inhibit most bacteria besides GpA and GpB strep.
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| What kind of hemolysis is caused my S.pyogenes and agalactiae? | beta hemolysis.
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