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Microbiology Media
Media selection
Question | Answer |
---|---|
5% Sheep Blood Agar is non-selective but differential for what? | Differentiates alpha, beta and nonhemolytic bacteria. |
Blood Agar is composed of what nutrient? | 5% sheep blood |
What types of organisms are supported by the blood agar? | All but the most fastidious organisms. Most gram pos and gram neg bacteria |
What is Brilliant Green differential for? | Lactose pos and Lactose neg bacteria. |
Lactose pos on Brilliant Green decreases pH and is what color? | yellow green |
on Brilliant Green what colors will be seen on Lactose neg? | pink colony with red zone in medium |
What type of bacteria will Brilliant Green inhibit? | most enteric bacteria |
What kind of agar is the Chocolate Agar? | Enriched |
How is a Chocolate Agar enriched? | It is a Blood agar base with hemoglobin, hemin and co enzyme nicotine adenine dinucleotide(NAD) |
what factor is hemin in a chocolate agar? | V Factor |
What factor is NAD in a chocolate agar? | X Factor |
The chocolate auger is enriched with X factor and V factor to support the growth of what? | H. influenzae and N. gonorrhaeae, plus most other gram pos and gram neg bacteria. |
How is Columbia CNA agar differential? | it will demonstrate alpha /beta/no hemolysis |
Columbia CNA agar is selective for what? | Gram pos bacteria. |
The Columbia CNA agar has the ability to grow what? | Staph and Strep. |
On the Columbia CNA agar what inhibits gram neg bacteria from growing? | Colistan and nalidix acid |
The EMB ager contains what two dyes? | Eosin Y and Methylene Blue |
The dyes in the EMB agar cause it to be selective by what? | The dyes inhibit gram pos rods. |
The EMB agar is differential how? | Gram neg lactose fermentation |
On the EMB agar what color will a lactose pos be. | Dark purple center, Lactose neg will not have a dark purple center. |
Hektoen Enteric Agar inhibits wh | |
Hektoen Enteric agar is differential for what? | Lactose pos and neg bacteria |
On the HE agar how do you differentiate between lactose pos and neg? | decreased pH will cause yellow colonies for L+ and green blue for L-. |
What type of bacteria will the HE agar inhibit? | most normal enteric bacteria. |
Selenite F broth is used to enrich what bacteria | Salmonella and other patogenic enteric bacteria |
In Selenite F broth what is used to inhibit normal flora? | sodium selenite |
The Thayer Martin Agar is selective for what? | N. gonorrrhoeae and some other Neisseria species. |
What is the TM agar made of | colistan to inhibit other gram neg Vancomycin to inhibit gram pos nystatin to inhibit yeast |
Modified TM contains what? | trimethoprim to inhibit Proteus. |
Thioglycollate Broth is used for what? | enrichment for most all bacteria |
Mac Conkey is selective for what? | enteric gram neg |
Mac Conkey differentiate Lactose fermenters how? | Lactose + is red while Lactose- is clear |
in the Mac Conkey agar what causes inhibition of gram + bacteria? | crystal violet dye and protein. |
What species is Mannitol salt selective for? | Staph |
In Mannitol Salt Agar what is used to inhibit gram- and other gram pos bacteria besides staph? | NaCl |
Mannitol fermenters and be differentiated from non fermenters be? | fermenters are yellow and non-fermenters are pink. |
PEA agar is used for? | same as CNA |
What does SXT stand for? | Strep Selective Agar. |
What kind of culture is most likely used with a SXT agar? | throat cultures |
What is the SXT agar composed of? | Sheep blood agar with trimethoprim-sulfa an antibiotic used to inhibit most bacteria besides GpA and GpB strep. |
What kind of hemolysis is caused my S.pyogenes and agalactiae? | beta hemolysis. |