molecular diagnostics
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each of the black spaces below before clicking
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| A suitable method to use for quantitation of HIV in patient blood is: bDNA. DNA sequencing. SDA. Southern Blot | bDNA
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| All of the following are clinical applications of FISH except Detection of chromosome microdeletions Detection of oncogenes in tumor cells Monitoring patients with sex-mismatched bone marrow transplants for engraftment | Detection of oncogenes in tumor cells
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| Labeled antibodies are used as probes to detect proteins in: Hybrid capture assay. Northern blot. Southern blot. Western blot. | Western Blot
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| RNA differs from DNA in that RNA: Contains thymine instead of uracil. Contains an H group instead of an OH group at its number 2 carbon. Is normally single-stranded. Is used to carry the genetic information of most viruses | Contains an H group instead of an OH group at its number 2 carbon.
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| Real-time PCR differs from traditional PCR in that: Primers are not necessary. The amplicon is detected as the reaction progresses. The amplicon is detected on a special fluorescent gel. The reaction requires hybridization probes | The reaction requires hybridization probes
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| Which of the following is NOT a component of a nucleotide? 5' carbon sugar Amino acid Nitrogenous base Phosphate group | Nitrogenous base
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| Which of the following is a step in the process of translation RNA polymerase binds to the promoter sequence Introns are removed from the transcript A string of A's are added to the 3' end of the mRNA | Introns are removed from the transcript.
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| Which of the following is true about gel electrophoresis of DNA DNA migrates toward the cathode. Increasing the voltage increases the resolution of DNA fragments separated in the gel | Increasing the voltage increases the resolution of DNA fragments separated in the gel.
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Created by:
Mwortman
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