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MLT Molecular

molecular diagnostics

QuestionAnswer
A suitable method to use for quantitation of HIV in patient blood is: bDNA. DNA sequencing. SDA. Southern Blot bDNA
All of the following are clinical applications of FISH except Detection of chromosome microdeletions Detection of oncogenes in tumor cells Monitoring patients with sex-mismatched bone marrow transplants for engraftment Detection of oncogenes in tumor cells
Labeled antibodies are used as probes to detect proteins in: Hybrid capture assay. Northern blot. Southern blot. Western blot. Western Blot
RNA differs from DNA in that RNA: Contains thymine instead of uracil. Contains an H group instead of an OH group at its number 2 carbon. Is normally single-stranded. Is used to carry the genetic information of most viruses Contains an H group instead of an OH group at its number 2 carbon.
Real-time PCR differs from traditional PCR in that: Primers are not necessary. The amplicon is detected as the reaction progresses. The amplicon is detected on a special fluorescent gel. The reaction requires hybridization probes The reaction requires hybridization probes
Which of the following is NOT a component of a nucleotide? 5' carbon sugar Amino acid Nitrogenous base Phosphate group Nitrogenous base
Which of the following is a step in the process of translation RNA polymerase binds to the promoter sequence Introns are removed from the transcript A string of A's are added to the 3' end of the mRNA Introns are removed from the transcript.
Which of the following is true about gel electrophoresis of DNA DNA migrates toward the cathode. Increasing the voltage increases the resolution of DNA fragments separated in the gel Increasing the voltage increases the resolution of DNA fragments separated in the gel.
Created by: Mwortman