Viral culture
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| Chicken embryo culture | historic method for viral culture, technically difficult and labor intensive
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| Cell types used for culture | Primary, diploid, continuous
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| Primary cell lines | derived directly from donor, rhesus monkey or rabbit kidney, 1-2 passages
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| Diploid cell lines | Prepared from animal tissue, lung or foreskin fibroblast, 20-50 passages, terminally differentiated-post mitotic
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| Continuous cell lines | single cell type propogated indefinately, derived from tumor cells, unlimited passage. A549 (human lung carcinoma), HEp-2
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| Cytopathic effect | Rounding up or detachment, lysis, nuclear swelling, cell fusion (syncytia)
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| Hemadsorption | hemagglutinin HA produced by some viruses binds erythrocytes, uses human type O, guinnea pig, chicken, performed on culture from resp samples
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| Fluorescent ab test | confirms CPE, some direct samples, CMV, HSV, respiratory viruses, enteroviruses
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| Shell vial culture | cells on coverslip in shell, can have multiple cell lines, centrifuged, faster results, use for CMV, HSV, ZVZ, chlamydia, resp viruses
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Created by:
kmaemom
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