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Viral culture

Chicken embryo culture historic method for viral culture, technically difficult and labor intensive
Cell types used for culture Primary, diploid, continuous
Primary cell lines derived directly from donor, rhesus monkey or rabbit kidney, 1-2 passages
Diploid cell lines Prepared from animal tissue, lung or foreskin fibroblast, 20-50 passages, terminally differentiated-post mitotic
Continuous cell lines single cell type propogated indefinately, derived from tumor cells, unlimited passage. A549 (human lung carcinoma), HEp-2
Cytopathic effect Rounding up or detachment, lysis, nuclear swelling, cell fusion (syncytia)
Hemadsorption hemagglutinin HA produced by some viruses binds erythrocytes, uses human type O, guinnea pig, chicken, performed on culture from resp samples
Fluorescent ab test confirms CPE, some direct samples, CMV, HSV, respiratory viruses, enteroviruses
Shell vial culture cells on coverslip in shell, can have multiple cell lines, centrifuged, faster results, use for CMV, HSV, ZVZ, chlamydia, resp viruses
Created by: kmaemom