Chapter 1
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| A chemical that alters tissue by stabilizing protein in such a way that the tissue is resistant to further change is the definition of...? | show 🗑
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| A fixative must change the soluble/insoluble contentsof the cell into soluble/insoluble substances so that those substances are not lost during subsequent processing steps? | show 🗑
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| show | Prevent putrefaction, prevent autolysis, maintain the proper relationship between cells and extracellular substances, enhance the refractive index, enhance staining.
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| List the actions of a fixative? | show 🗑
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| Name the two methods of stabilizing proteins (fixation)? | show 🗑
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| show | Heat (microwave)and Desiccation (air-drying)
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| What is the primary method of protein stabilization (fixation)? | show 🗑
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| show | Temperature (increase in temp.increases rate which increases autolysis) , size of tissue (3mm thick), volume ratio 15-20 times tissue volume), time (length of time from incision to fixative submersion time before processing)
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| What is the volume ratio between fixative and tissue? | show 🗑
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| List four things to consider when choosing a fixative? | show 🗑
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| show | Inward
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| What fioxative penetrates faster than any other common fixative ingredient? | show 🗑
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| show | Indefinitely
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| show | Wet
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| What can be the effect on tissue if the solution drops below pH 4.0? | show 🗑
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| What is the pH range of most fixatives? | show 🗑
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| show | 7.2 to 7.4
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| show | Alkali (base)
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| show | Acidic
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| Soultions that have a pH of 7.0 are considered alkali (base), acidic, or neutral? | show 🗑
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| Is the pH of a solution more important for light microscopy or electron microscopy? | show 🗑
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| show | Osmolality
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| What is the osmolality of body fluids? | show 🗑
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| If a cell in in a solution that is more concentrated than the cell cytosol(contains more particles) is this solution considered hypertonic, hypotonic or isotonic to the cell? | show 🗑
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| If a cell is in a solution that is less concentrated than the cell cytosol(contains less particles) is this solution considered hypertonic, hypotonic or isotonic to the cell? | show 🗑
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| Fluids into which normal cells can be placed without causing either swelling or shrinkageare considered what? | show 🗑
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| show | Isotonic
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| This classification of a fixative changes the tertiary structure of a protein? | show 🗑
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| Chemicals combine with the protein molecules to change them. This means the protein is made insoluble by the addition of the fixative and then becomes immobilized, remaining at its original location in the cell. What is this the defintion of? | show 🗑
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| show | Nonadditive Fixatives
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| show | Nonadditive Fixatives
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Review the information in the table. When you are ready to quiz yourself you can hide individual columns or the entire table. Then you can click on the empty cells to reveal the answer. Try to recall what will be displayed before clicking the empty cell.
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You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
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