Mycobacterium tuberculosis and Other Nontuberculosis Mycobacteria
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
|
|
||||
|---|---|---|---|---|---|
| Mycobacterium tuberculosis | obligate pathogenic bacterial species in the family Mycobacteriaceae and the causative agent of tuberculosis; the etiologic agent of tuberculosis in humans. Humans are the only reservoir for the bacterium.
🗑
|
||||
| Mycobacterium bovis | slow-growing aerobic bacterium; etiologic agent of TB in cows and rarely in humans; both cows and humans can serve as reservoirs; humans can also be infected by the consumption of unpasteurized milk.
🗑
|
||||
| slow growing species of Mycobacterium that are photochromogens | Mycobacterium kansasii, Mycobacterium marinum, Mycobacterium asiaticum, and Mycobacterium simiae. Mycobacterium szulgai is a photochromogen when grown at 24 degrees
🗑
|
||||
| slow growing species of Mycobacterium that are scotochromogens | Mycobacterium gordonae, Mycobacterium scrofulaceum, Mycobacterium xenopi (ypung cultures may be nonchromogenic . Mycobacterium szulgai is a scotochromogen when grown at 37 degrees
🗑
|
||||
| rapid growing species of Mycobacterium | Mycobacterium phlei, Mycobacterium smegmatis, Mycobacterium fortuitum, Mycobacterium chelonae,
🗑
|
||||
| Mycobacterium leprae | also known as Hansen’s bacillus spirilly, mostly found in warm tropical countries, is a Gram-positive bacterium that causes leprosy. It is an intracellular, pleomorphic, acid-fast, pathogenic bacterium
🗑
|
||||
| Middlebrook mediums | serum albumin-based clear agar media used to support the growth of mycobacteria.
🗑
|
||||
| Tween 80 test | useful in identifying Mycobacterium kansasii (which can produce positive results within 3-6 hours); to differentiate two scotochromogens with similar appearing colonies, A positive = the liquid, not the cells, light orange to pink or red.
🗑
|
||||
| Iron Uptake test | distinguses M. chelonae which is negative from rapid growers; used to detect thse mycobacteria capable of converting ferric ammonium citrate to iron oxide
🗑
|
||||
| Arylsulfatase test | The rate by which arylsulfatase enzyme breaks down phenolphthalein disulfate into phenolphthalein and other salts; used to identify potentially pathogenic rapid growers; Slow growing are positive in the 14 day arylsulfatase test.
🗑
|
||||
| Pyrazinamidase test | Pyrazinamide (PZA) is an anti-tuberculosis drug M. bovis; used to differentiate between the species, but susceptibility testing becomes complicated, works best at a low pH in vitro; prevents growth of some mycobacteria.
🗑
|
||||
| Tellurite test | the ability of mycobacterial species to reduce tellurite in 3 to 4 days is used to distinguish members of M. avium complex from most other non-chromogenic species. All rapid growers reduce tellurite in 3 days.
🗑
|
||||
| Urease test | negative: orange/yellow; positive: bright pink; diamide of carbonic acid, hydrolyzed with release of ammonia & carbon dioxide; ammonia combines w/carbon dioxide & water to form ammonium carbonate; turns medium alkaline, phenol red- orange/yellow-pink
🗑
|
||||
| NAP test | a precursor in the synthesis of chloramphenicol; selectively inhibits the M. tuberculosis complex.
🗑
|
||||
| T2H | distinguishes M. bovis from M. tuberculosis; M. bovis is susceptible to lower concentrations of T2H than MTB,; variability in inhibition exists, depending on the concentration of the inhibitory agent and the temperature of incubation
🗑
|
Review the information in the table. When you are ready to quiz yourself you can hide individual columns or the entire table. Then you can click on the empty cells to reveal the answer. Try to recall what will be displayed before clicking the empty cell.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
Created by:
luceroapril
Popular Laboratory Science sets