Haemophilus and Other Fastidious Gram-Negative Bacilli
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
|
|
||||
|---|---|---|---|---|---|
| Haemophilus *colony morphology* | pleomorphic, nonmotile, facultatively anaerobic
🗑
|
||||
| Haemophilus *gram stain results* | gram-negative cocobacilli or rods
🗑
|
||||
| Haemophilus *testing results* | ferment-carbohydrates, generally oxidase- and catalase-positive, reduce nitrates to nitirtes
🗑
|
||||
| Haemophilus *epidemiology* | obligate parasites on the mucous membranes of humans and animals, nonpathogenic or produce opportunistic infections
🗑
|
||||
| Haemophilus influenza *colony morphology* | small, nonmotile, facultatively anaerobic, pleomorphic
🗑
|
||||
| Haemophilus influenza *gram stain results* | gram-negative rod
🗑
|
||||
| Haemophilus influenza *testing results* | indole, urease, and ornithine secarboxylase
🗑
|
||||
| Haemophilus influenza *virulence factors* | capsule, IgA, adherance by fimbriae, and other structures, outer membrane proteins
🗑
|
||||
| Haemophilus influenza *epidemiology* | nasopharynx, upper respiratory tract
🗑
|
||||
| Haemophilus influenza *infections* | meningitis, epiglottitis, bacterial tracheitis
🗑
|
||||
| Define X factor | growth factor that some Haemophhilus spp. require in media for growth; also known as hemin or hematin
🗑
|
||||
| Define V factor | growth factor that some Haemophhilus spp. require in media for growth; also known as nicotinamide adenosine dinucleotide (NAD)
🗑
|
||||
| Porphyrin test *principle* | contains hemoglobin as a source of hemin (X factor), supplement which provides NAD (V factor), vitamins, amino acids, coenzymes, dextrose, and other nutrients provides an accurate means of determining X factor
🗑
|
||||
| Porphyrin test *reagents* | Casein Peptone,
Delta-aminolevulinic Acid,
Monopotassium Phosphate,
Hemoglobin Solution,
Dipotassium Phosphate,
Sodium Chloride,
Glucose,
L-Glutamine,
Hydrochloride,
🗑
|
||||
| Porphyrin test *results reporting* | Positive Test - orange red fluorescence
Negative Test - no fluorescence
🗑
|
||||
| HACEK group and list of members | Haemophilus,
Aggregatibacter aphrophilus
(formerly Actinobacillus actinomycetemcomitans),
Cardiobacterium hominis,
Eikenella corrodens,
Kingella sp,
🗑
|
||||
| Aggregatibacter aphrophilus *colony morphology* | star shaped centers of colonies
🗑
|
||||
| Aggregatibacter aphrophilus *gram stain results* | gram-negative bacilli
🗑
|
||||
| Aggregatibacter aphrophilus *testing results* | catalase-positive oxidase variable do not grow on MAC negative for X and V growthfactors, urease, indole, esculin, and citrate
🗑
|
||||
| Aggregatibacter aphrophilus *virulence factors* | collagenase and a leukotoxin
🗑
|
||||
| Aggregatibacter aphrophilus *epidemiology* | dental plaque and gingival scrapings
🗑
|
||||
| Aggregatibacter aphrophilus *infections* | endocarditis
🗑
|
||||
| Cardiobacterium hominis *colony morphology* | form rosettes, swelling, long filaments, or sticklike structures
🗑
|
||||
| Cardiobacterium hominis *gram stain results* | gram-negative bacilli some false gram-positive
🗑
|
||||
| Cardiobacterium hominis *testing results* | oxidase-positive, catalase-negative, and indole-positve. negative for urase, nitrate, gelatin, esculin
🗑
|
||||
| Cardiobacterium hominis *epidemiology* | attachment to heart valves usually damaged or prosthetic, normal biota of the oral cavity and nose
🗑
|
||||
| Cardiobacterium hominis *infections* | endocarditis, infect aortic valve.
🗑
|
||||
| Eikenella corrodens *colony morphology* | nonhemolytic on SBA, slight gree effect secondaryto growth, nonmotile
🗑
|
||||
| Eikenella corrodens *gram stain results* | gram-negative coccobacilli
🗑
|
||||
| Eikenella corrodens *testing results* | oxidase-positive, catalase-negative
🗑
|
||||
| Eikenella corrodens *virulence factors* | resistant to clindamycin and narrow-spectrum cephalosporins
🗑
|
||||
| Eikenella corrodens *epidemiology* | attachment to heart valves usually damaged or prosthetic, normal biota of the oral and bowel cavity
🗑
|
||||
| Eikenella corrodens *infections* | endocarditis, periodontitis, meningitis, empyema, pneumonia, osteomyelitis, arthritis, and postoperative tissue infections
🗑
|
||||
| Kingella *colony morphology* | nonmotile, large white β-hemolytic colonies on SBA, smooth, convex
🗑
|
||||
| Kingella *gram stain results* | gram-negative bacilli
🗑
|
||||
| Kingella *testing results* | oxidase-positive, catalase-negative, negative for urease, indole, esculin, gelatin and citrate
🗑
|
||||
| Kingella *epidemiology* | attachment to heart valves usually damaged or prosthetic, normal biota of the oral cavity, upper respiratory tract
🗑
|
||||
| Kingella *infections* | endocarditis, degenerative joint and bone infections
🗑
|
||||
| Capnocytophaga *colony morphology* | faculatatively anaerobic, thin often fusiform, spindle shapedgliding motility on solid surfaces, nonhemolytic
🗑
|
||||
| Capnocytophaga *gram stain results* | gram-negative bacilli
🗑
|
||||
| Capnocytophaga *testing results* | ferment sucrose, glucose, maltose, and lactose, negative for most biochemical reactions, may reduce nitrates and hydrolyze esculin
🗑
|
||||
| Capnocytophaga *virulence factors* | resistant to aminoglycosides
🗑
|
||||
| Capnocytophaga *epidemiology* | normal inhabitant of oral cavity
🗑
|
||||
| Capnocytophaga *infections* | periodontis and endocarditis
🗑
|
||||
| Pasteurella *colony morphology* | nonmotile, facultative, anaerobic, ovoid, filamentous
🗑
|
||||
| Pasteurella *gram stain results* | gram-negative cocbacilli
🗑
|
||||
| Pasteurella *testing results* | catalase and oxidae positive ferment glucose with weak to moderate acid production without gas.
🗑
|
||||
| Pasteurella *virulence factors* | capnophilic
🗑
|
||||
| Pasteurella *epidemiology* | infected animals
🗑
|
||||
| Pasteurella *infections* | septicemia, arthritis, endocarditis, osteomyelitis, meningitis
🗑
|
||||
| Brucella *colony morphology* | aerobic, nonmotile,
🗑
|
||||
| Brucella *gram stain results* | gram-negative coccobacilli or bacilli
🗑
|
||||
| Brucella *testing results* | oxidase and catalase positive urease positive within 2 hoursgrow on SBA amd CHOC agar isolated and modified on Thayer-Martin or Martin-Lewis
🗑
|
||||
| Brucella *virulence factors* | unencapsulated
🗑
|
||||
| Brucella *epidemiology* | zoonotic disease found throughput the world
🗑
|
||||
| Brucella *infections* | acute, subchronic, and chronic
🗑
|
||||
| Francisella *colony morphology* | small, nonmotile, non-spore-forming, aerobic, gray-white, raised smooth appearance
🗑
|
||||
| Francisella *gram stain results* | gram-negative bacilli or coccoid
🗑
|
||||
| Francisella *testing results* | oxidase, urease, and satellite or X and V test negative and weakly positive for catalase and β-lactamase activity
🗑
|
||||
| Francisella *epidemiology* | zoonotic disease through ingestion, inhalation, arthropod bite, or contact with infected tissue
🗑
|
||||
| Francisella *infections* | ularemia infections, ulceroglandula, pneumonia
🗑
|
||||
| infections caused by Legionella | Legionnaires disease, pontiac fever
🗑
|
||||
| Bordetella *colony morphology* | areobic
🗑
|
||||
| Bordetella *gram stain results* | gram-negative bacilli or coccobacilli
🗑
|
||||
| Bordetella *testing results* | do not ferment carbohydrates, oxidize amino acids, relatively inactive in biochemical tests, produce catalase
🗑
|
||||
| Bordetella *virulence factors* | filamentous hemagglutinin and pertacin, pertussis toxin, adenylate cyclase toxin, tracheal cytotoxin
🗑
|
||||
| Bordetella *epidemiology* | respiratory tract
🗑
|
||||
| Bordetella *infections* | classic pertussis
🗑
|
||||
| Bordetella pertussis *colony morphology* | small, nonmotile, obligately aerobic
🗑
|
||||
| Bordetella pertussis *gram stain results* | gram-negative coccobacilli
🗑
|
||||
| Bordetella pertussis *testing results* | oxidase-positive, urease-negative, nitrate-negative,
🗑
|
||||
| Bordetella pertussis *virulence factors* | filamentous hemagglutinin and pertacin, pertussis toxin, adenylate cyclase toxin, tracheal cytotoxin
🗑
|
||||
| Bordetella pertussis *epidemiology* | human disease no animal reservoir or vector
🗑
|
||||
| Bordetella pertussis *infections* | classic pertussis "whooping cough"
🗑
|
Review the information in the table. When you are ready to quiz yourself you can hide individual columns or the entire table. Then you can click on the empty cells to reveal the answer. Try to recall what will be displayed before clicking the empty cell.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
Created by:
luceroapril
Popular Laboratory Science sets