Use of Colonial Morphology for the Presumptive Identification of Microorganisms
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| Define plate reading | interpretation of Gram-stained smears from clinical specimens and microbial colonies. Provides initial identification of microorganism by microscopic characterization, description of physical growth characteristics of microorganisms on laboratory medium
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| Define hemolysis | dissolution or breaking apart pertaining to red blood cells; a reaction, especially caused by enzymatic or toxin activity of bacteria, observed in the medium immediately surrounding or underneath the colony.
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| List and explain all types of hemolysis | α-Hemolysis- is a partial lysing of erythrocytes around an under the colony that results in a green discoloration
β-Hemolysis- is a complete clearing of erythrocytes around or under the colonies because of the because of the complete lysis of RBCs.
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| Define colony morphology and list descriptive terms used and explain or draw them | colony characteristics and form.
size
form or margin
elevation
density
color
consistency
pigment
odor
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| Define pigment and give examples | coloring matter or substance:
green, sometimes metallic (P. aeruginosa)
brick-red (Serratia marcescens)
blue (Kluyvera spp.)
purple ( Chromobacterium violaceum)
brown-black (Prevotella melaninogenica)
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| Define odor and give examples | the odor is determined when the lid of the culture plate is removed and it dissipates into the surrounding area
old socks(S.aureus);
grapelike(P.aeruginosa);
putrid(P.mirabilis);
mousy(Haemophilus spp);
freshly plowed field (Nocardia spp)
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