Histology ASCP Special Stains
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each of the black spaces below before clicking
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| Substances attracting basic dyes | show 🗑
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| show | Acidophillic
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| show | Chromophore
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| A benzene derivative containing chromophoric groups | show 🗑
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| Ionizing group required to enable the dye to link firmly to tissue | show 🗑
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| show | Cationic dyes
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| show | Anionic dyes
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| Progressive staining | show 🗑
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| show | Mordant
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| show | The tissue is overstained and then differentiated, or decolorized, until only the desired element is left stained.
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| show | Weak acid solutions
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| Acidic, or anionic dyes, are differentiated by: | show 🗑
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| Reagents: Hematoxylin, Ethanol, Ammonium Alumnium Sulfate, Distilled Water, Mercuric Oxide | show 🗑
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| show | Harris Hematoxylin, Mayer Hematoxylin, Ehrlich Hematoxylin, Gill Hematoxylin
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| show | Delafield Hematoxylin
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| Mordant: Aluminum Ripner: Light and air | show 🗑
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| Reagents: Hematoxylin, Distilled Water, Sodium Iodate, Ammonium or potassium Aluminum Sulfate, citric Acid, chloral Hydrate | show 🗑
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| show | Ehrlich Hematoxylin
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| show | Gill Hematoxylin
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| Mucin, especially goblet cells, will be stained by which hematoxylin | show 🗑
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| show | Weigert Hematoxylin
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| Mordant and oxidizer: Ferric Chloride | show 🗑
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| show | Celestine Blue
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| show | Scott Solution
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| Fixatives that cause loss of basophilia | show 🗑
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| Demonstration of DNA | show 🗑
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| Any fixative but Bouin | show 🗑
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| show | Feulgen Reaction
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| DNA: Reddish Purple Cytoplasm (if counterstained): Light Green | show 🗑
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| Differentiation between DNA and RNA | show 🗑
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| 10% NFB preferred. B5, Helly or Zenker satisfactory. | show 🗑
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| Reagents: Acetic Acid, Sodium Acetate, Methyl Green Staining Solution, Methyl Green-Pyronin Y Staining Solution | show 🗑
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| show | Methyl Green-Pyronin Y
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| Process in which a dye forms other dyes spontaneously | show 🗑
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| show | May-Grunwald Giemsa
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| show | May-Grunwald Giemsa
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| show | May-Grunwald Giemsa
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| show | May-Grunwald Giemsa
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| Jenner Solution, Giemsa Solution, Acetic Water | show 🗑
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| Nuceli: Blue Cytoplasm of leukocytes: Pink, gray or blue Bacteria: Blue | show 🗑
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| Demonstration of polysaccharides, neutral mucosubstances and basement membranes | show 🗑
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| show | PAS, Alcian Blue pH 2.5, Alcian Blue pH 1.0
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| Control: Kidney | show 🗑
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| Reagents: Periodic Acid, Hydrochloric Acid, Potassium Metabisulfite, Schiffs | show 🗑
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| show | PAS
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| show | PAS D, Best Carmine
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| 10% NBF, formalin alcohol or absolute alcohol | show 🗑
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| Control: Liver | show 🗑
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| show | PAS D
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| show | PAS D
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| Absolute Alcohol preferred. Carnoy and Bouin satisfactory. | show 🗑
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| show | Best Carmine
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| show | Best Carmine
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| Demonstration of epithelial mucin | show 🗑
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| show | Mayer Mucicarmine, Alcian Blue pH 2.5, Alcian Blue pH 1.0, Muller-Mowry Colloidal Iron
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| show | Mayer Mucicarmine
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| Mucin: Deep rose to red Capsule of cryptococcus: Deep rose to red Nuclei: Black Other: Blue or yellow | show 🗑
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| Demonstration of acid mucopolysaccharides | show 🗑
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| show | Alcian Blue pH 2.5
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| Sulfated Mucosubstances: Dark Blue Hyaluronic Acid: Dark Blue Sialomucins: Dark Blue Background: Pink to red Nuclei: Red | show 🗑
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| Demonstration of sulfated mucosubstances | show 🗑
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| show | Alcian Blue pH 1.0
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| show | Alcian Blue pH 1.0
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| Differentiation of epithelial and connective tissue mucins | show 🗑
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| show | Alcian Blue with Hyaluronidase
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| show | Alcian Blue with Hyaluronidase
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| Without digestion: acid mucopolysaccharides and sialomucins: Deep Blue With digestion: mucosubstances containing hyaluronic acid and chondroitin sulfates: Marked loss of staining | show 🗑
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| Differentiation between neutral and acidic mucosubstances | show 🗑
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| 10% NBF or Zenker | show 🗑
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| Reagents; Acetic Acid, Alcian Blue,Periodic Acid, Sodium Metabisulfite | show 🗑
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| show | Alcian Blue-PAS-Hematoxylin
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| Demonstration of carboxylated and sulfated mucopolysaccharides and glycoproteins | show 🗑
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| Chemical reaction to demonstrate iron bound to tissue | show 🗑
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| 10% NBF, Carnoy Solution or alcoholic formalin | show 🗑
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| Avoid chromate fixatives | show 🗑
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| Reagents: Ferric chloride, Muller Colloidal Iron, Potassium Ferrocyanide, Hydrochloric Acid, Acetic Acid, Nuclear Fast Red | show 🗑
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| show | Muller-Mowry Colloidal Iron
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| show | Mayer Mucicarmine, Muller-Mowry Colloidal Iron, Russell Modification of the Movat Pentachrome Stain
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| Demonstration of Amyloid | show 🗑
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| Alcohol or Carnoy is preferred. 10% NBF, Bouin or Zenker satisfactory. | show 🗑
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| Control: Sections containing amyloid | show 🗑
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| Reagents; 80% Alcohol saturated with Sodium Chloride, Alkaline salt solution, Congo Red | show 🗑
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| Amyloid: Deep pink to red Elastic tissue: Pale Pink Nuclei: Blue | show 🗑
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| Birefringent bright apple green | show 🗑
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| show | Crystal Violet, Prussian Blue Stain, Turnbull Blue, Von Kossa
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| Amyloid: Purplish Violet Other: Blue | show 🗑
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| Reagents: Thioflavine T, Acetic Acid, Mayer Hematoxylin | show 🗑
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| show | Thioflavine T
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| show | Masson Trichrome, Gomori 1-Step Trichrome
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| show | Masson Trichrome
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| Internal Control | show 🗑
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| show | Masson Trichrome
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| Nuclei: Black Cytoplasm, keratin, muscle fibers; Red Collagen and Mucin: Blue | show 🗑
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| Any well fixed tissue. | show 🗑
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| Bouin used as a mordant to intensify color reactions. | show 🗑
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| show | Gomori 1-Step Trichrome
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| show | Gomori 1-Step Trichrome
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| Considered primary connective tissue stain, rarely used, except as counterstain. | show 🗑
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| show | Van Gieson Picric Acid-Acid Fuchsin
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| show | Van Gieson Picric Acid-Acid Fuchsin
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| show | Van Gieson Picric Acid-Acid Fuchsin
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| show | Verhoeff, Gomori Aldehyde Fuchsin, Russell Modification of the Movat Pentachrome Stain
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| Reagents: Lugol Iodine, Ferric Chloride, Alcoholic Hematoxylin, Verhoeff, van Gieson, Sodium Thiosulfate | show 🗑
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| show | Verhoeff
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| Control: Aorta or muscular artery | show 🗑
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| Elastic Fibers = Deep blue to purple Other = Green | show 🗑
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| Reagents: Aldehyde Fuchsin, Light Green | show 🗑
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| show | Russell Modification of the Movat Pentachrome Stain
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| show | Russell Modification of the Movat Pentachrome Stain, Mallory PTAH
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| show | Russell Modification of the Movat Pentachrome Stain
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| show | Russell Modification of the Movat Pentachrome Stain
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| Control: Lung, skin or colon | show 🗑
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| Reagents: Alcian Blue, Alkaline Alcohol, Iodine-Iodide, Absolute Alcoholic Hematoxylin, Ferric Chloride, Sodium Thiosulfate, Crocein Scarlet-Acid Fuchsin, Phosphotungstic Acid, Alcoholic Safran | show 🗑
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| Nuclei = black Elastic fibers = black Ground substance and mucin = Blue Collagen = Yellow Fibrinoid, fibrin = Intense Red Muscle = Red | show 🗑
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| show | Russell Modification of the Movat Pentachrome Stain
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| Metallic impregnation step | show 🗑
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| Reduction step in silver impregnation | show 🗑
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| When bound metallic silver is treated with gold chloride and the color of the impregnated component is changed from brown to black | show 🗑
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| Most frequently used oxidizers | show 🗑
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| Demonstration of reticular fibers | show 🗑
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| show | Gomori Stain, Gordon and Sweets
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| Ferric ammonium sulfate is sensitizer | show 🗑
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| Reducer in all reticular fiber silver methods | show 🗑
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| Reagents: Silver Nitrate, Potassium Hydroxide, Ammoniacal Silver, Potassium Permanganate, Potassium Metabisulfite, Ferric Ammonium Sulfate, Formalin, Gold Chloride, Sodium Thiosulfate, Nuclear Fast Red | show 🗑
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| show | Gomori Stain
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| Demonstration of muscle cross-striations | show 🗑
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| Demonstration of glial fibers | show 🗑
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| Demonstration of myelin | show 🗑
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| Zenker is preferred. 10% NBF is satisfactory. | show 🗑
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| Control: skeletal or cardiac muscle | show 🗑
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| show | Mallory PTAH
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| show | Mallory PTAH
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| Cross striations, fibrin = Blue Nuclei = Blue Collagen = Red-brown | show 🗑
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| Demonstration of basement membranes | show 🗑
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| Avoid mercuric fixatives | show 🗑
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| show | Periodic Acid-Methenamine Silver
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| show | Periodic Acid-Methenamine Silver
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| Demonstration of Lipids | show 🗑
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| show | Oil Red O, Sudan Black B
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| show | Oil Red O, Sudan Black B, Cajal Stain
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| show | Oil Red O
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| Fat = Red Other - Dependent on method | show 🗑
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| Avoid alcoholic fixatives | show 🗑
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| show | Sudan Black B
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| show | Sudan Black B
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| Reagents: Osmium Tetroxide, Periodic Acid | show 🗑
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| show | Osmium Tetroxide
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| Demonstration of mast cells | show 🗑
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| When tissue stains a different color from the dye solution and the rest of the tissue | show 🗑
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| show | Metachromasia
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| Control: Section containing mast cells | show 🗑
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| show | Toluidine Blue
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| Mast cells = Deep rose-violet Background = Blue | show 🗑
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| Mast cells = orange-red | show 🗑
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| show | Cresyl Echt Violet
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| Loss of Nissl substance | show 🗑
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| Control: Spinal cord | show 🗑
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| Reagents: Cresyl Echt Violet, Balsam-Xyelene | show 🗑
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| Nissl Substance = Blue to purple Nuclei = Blue to purple Background = colorless | show 🗑
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| show | Bodian Method, Holmes Silver Nitrate Method, Bielschowsky-PAS, Churukian Bielschowsky, Sevier-Munger, Thioflavin S
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| show | Wallerian degeneration
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| show | Bodian Method
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| Reagents: Protargol, Reducing Solution (Hydroquinone, Formaldehyde), Gold Chloride, Oxalic Acid, Sodium Thiosulfate, Aqua Regia (Hydrochloric acid and Nitric Acid), Aniline Blue | show 🗑
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| Nerve Fibers = Black Nuclei = Black Background = Light gray or blue | show 🗑
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| Control: Cerebral cortex | show 🗑
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| show | Holmes Silver Nitrate Method
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| show | Holmes Silver Nitrate Method
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| Demonstration of senile plaques | show 🗑
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| show | Bielschowsky-PAS, Churukian Bielschowsky, Sevier-Munger, Thioflavin S
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| show | Bielschowsky-PAS
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| show | Bielschowsky-PAS
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| Reagents: Silver Nitrate, Nitric Acid, Developer (Formaldehyde, Citric Acid, Nitric Acid), Ammonium Hydroxide, Sodium Thiosulfate | show 🗑
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| Axons = Brown to black Cytoplasmic neurofibrils = Brown to Black Neurofibrillary tangles and plaques = Dark brown or black Neuromelanin = Black Lipofuchsin = Brown or black Background = Yellow | show 🗑
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| show | Sevier-Munger
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| show | Sevier-Munger
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| show | Thioflavin S
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| show | Thioflavin S
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| show | Congo Red, Thioflavin T, Thioflavin S, Auramine-Rhodamine, Alizarin Red S
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| Glial Fibers = Blue Nuclei = Blue Neurons = Salmon Myelin = Blue | show 🗑
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| show | Holzer Method
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| Glial Fibers = Blue Background = Very pale blue to colorless | show 🗑
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| show | Cajal Stain
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| show | Cajal Stain
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| show | Cajal Stain
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| show | Weil Method, Luxol Fast Blue, Luxol Fast Blue-PAS-Hematoxylin
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| Control = Medulla | show 🗑
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| show | Weil Method
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| show | Weil Method
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| show | Luxol Fast Blue
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| show | Luxol Fast Blue
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| show | Luxol Fast Blue-Cresyl Echt Violet Stain
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| show | Luxol Fast Blue-Holmes Silver Nitrate Method
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| Demonstration of fungi | show 🗑
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| show | Luxol Fast Blue-PAS-Hematoxylin
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| Reagents: Luxol Fast Blue, Lithium Carbonate, Schiffs, Periodic Acid, Harris Hematoxylin | show 🗑
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| show | Luxol Fast Blue-PAS-Hematoxylin
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| Demonstration of acid-fast mycobacteria | show 🗑
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| show | Kinyoun, Ziehl-Neelsen Method, Fite
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| Control: Tissue containing acid-fast organisms | show 🗑
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| Reagents: Kinyoun Carbol-Fuchsin, Acid Alcohol, Methylene Blue | show 🗑
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| Acid fast bacteria = Bright Red Background = Light Blue | show 🗑
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| Use Millipore-filtered water in water bath | show 🗑
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| show | Ziehl-Neelsen Method
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| show | Churukian Ziehl-Neelsen Method
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| Demonstration of Mycobacterium leprae | show 🗑
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| show | Fite
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| show | Fite
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| show | Fite
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| show | Auramine-Rhodamine
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| Reagents: Auramine O-Rhodamine B solution, Acid Alcohol, Eriochrome Black T | show 🗑
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| show | Auramine-Rhodamine
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| Demonstration of gram-positive and gram-negative bacteria | show 🗑
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| show | Gram
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| Reagents: Crystal Violet, Gram Iodine, Basic Fuchsin, Gallego Solution, Picric Acid-Acetone | show 🗑
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| Gram positive = Blue Gram negative = Red Nuclei = Light Red Background = Yellow | show 🗑
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| show | Diff-Quik Giemsa, Alcian Yellow-Toluidine Blue Method, Warthin-Starry, Steiner and Steiner
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| show | Diff-Quik Giemsa, Alcian Yellow-Toluidine Blue Method, Warthin-Starry, Steiner and Steiner
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| show | Diff-Quik Giemsa
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| H Pylori = Dark Blue Other bacteria = Blue Nuclei = Dark Blue Cytoplasm = Pink | show 🗑
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| show | Alcian Yellow-Toluidine Blue Method
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| H Pylori = Blue Mucin = Yellow Background = Pale Blue | show 🗑
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| 10% NBF, Bouin or Zenker. | show 🗑
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| show | Hotchkiss-McManus PAS (PAS F), Chromic Acid-Schiff (CAS), Gridley, Grocott-Methenamine Silver Nitrate (GMS)
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| Reagents: Periodic Acid, Schiffs, Hydrochloric Acid, Sodium Metabisulfite, Sulfurous Acid Rinse, Fast Green | show 🗑
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| Fungi = Rose Background = Green | show 🗑
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| show | Chromic Acid-Schiff (CAS)
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| show | Chromic Acid-Schiff (CAS)
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| Reagents: Chromic Acid, Schiffs, Aldehyde Fucchsin, Metanil Yellow | show 🗑
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| show | Gridley
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| show | Grocott-Methenamine Silver Nitrate (GMS)
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| Reagents: Chromic Acid, Silver Nitrate, Methenamine, Borax, sodium Bisulfite, Gold Chloride, Sodium Thiosfulfate, Light Green | show 🗑
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| Fungi = Black Mucin = Taupe to dark gray Background = Green | show 🗑
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| Demonstration of spirochetes | show 🗑
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| Control: Tissue containing spirochetes | show 🗑
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| show | Warthin-Starry
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| Spirochetes = Black Other bacteria = Black Background = Pale yellow to light brown | show 🗑
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| show | Dieterle Method, Steiner and Steiner
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| Reagents: Alcoholic Uranyl Nitrate, Alcoholic Gum Mastic, Silver Nitrate, Developer (Hydroquinone, Sodium Sulfite, Acetone, Formaldehyde, Pyridine), Formic Acid | show 🗑
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| show | Dieterle Method
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| show | Dieterle Method, Steiner and Steiner
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| Reagents: Uranyl Nitrate, Silver Nitrate, Reducing Solution (Gum Mastic, Hydroquinone, Absolute Alcohol) | show 🗑
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| Spirochetes = Dark brown to black H Pylori - Dark Brown to black Legionella = Dark Brown to Black Other nonfilamentous bacteria = Dark Brown to Black Background = Light Yellow | show 🗑
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| show | Artifact pigment
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| List exogenous pigments | show 🗑
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| List endogenous pigments that are hematogenous | show 🗑
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| show | Lipofuscin and ceroid
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| List endogenous pigments that are nonhematogenous and nonlipidic | show 🗑
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| show | Argyrophil
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| show | Argentaffin
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| Demonstration of ferric iron | show 🗑
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| Control: Tissue containing ferric iron | show 🗑
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| Reagents: Potassium Ferrocyanide, Hydrochloric Acid Solution, Nuclear Fast Red | show 🗑
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| show | Prussian Blue Stain
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| show | Turnbull Blue
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| Control: Tissue containing ferrous iron | show 🗑
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| Reagents: Hydrochloric Acid, Potassium Ferricynaide, Acetic Acid, Nuclear Fast Red | show 🗑
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| Ferrous Iron = Blue Background = Pink-red | show 🗑
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| Demonstration of melanin | show 🗑
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| Control: Tissue containing melanin or argentaffin granules | show 🗑
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| show | Schmorl Technique
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| show | Schmorl Technique
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| Demonstration of formalin pigment | show 🗑
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| show | Schmorl Technique, Fontana-Masson
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| show | Fontana-Masson
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| Reagents: Silver Nitrate, Fontana Silver, Gold Chloride, Sodium Thiosulfate, Nuclear Fast Red | show 🗑
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| Melanin = Black Argentaffin granules = Black Nuclei = Pink | show 🗑
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| show | Grimelius, Churukian-Schenk
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| show | Grimelius, Churukian-Schenk
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| Reagents: Silver Nitrate, Acetic Acid, Sodium Acetate, Acetic Acid-Sodium Acetate Buffer, Reducing Solution (Hydroquinone, Sodium Sulfite), Nuclear Fast Red | show 🗑
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| Argentaffin Granules = Dark brown to black Argyrophil Granules = Dark brown to black Nuclei = Red Background = Pale yellow-brown | show 🗑
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| Reagents: Citric Acid, Acidified Water, Silver Nitrate, Reducing Solution (Sodium Sulfite, Hydroquinone), Nuclear Fast Red | show 🗑
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| Argyrophil granules = Black Argentaffin granules = Black Nuclei = Red Background = Yellow-Brown | show 🗑
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| show | Gomori Methenamine-Silver
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| show | Gomori Methenamine-Silver
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| Control: Tissue containing urates | show 🗑
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| Reagents: Silver Nitrate, Methenamine Solution, Sodium Borate, Sodium Thiosulfate, Light Green | show 🗑
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| show | Gomori Methenamine-Silver
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| Demonstration of bilirubin | show 🗑
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| Control: Tissue containing bile | show 🗑
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| Reagents: Ferric Chloride, Fouchet Reagent, van Gieson | show 🗑
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| Bile or bilirubin = Emerald green to olive drab Background = Yellow | show 🗑
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| show | Von Kossa, Alizarin Red S
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| show | Von Kossa, Alizarin Red S
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| show | Von Kossa
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| show | Von Kossa
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| show | Alizarin Red S
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| show | Alizarin Red S
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| show | Alizarin Red S
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| show | Rhodanine
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| show | Rhodanine
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| Reagents: Rhodanine Solution, Mayer Hematoxylin, Sodium Borate | show 🗑
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| Copper = Bright red to red yellow Nuclei = Light Blue | show 🗑
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Review the information in the table. When you are ready to quiz yourself you can hide individual columns or the entire table. Then you can click on the empty cells to reveal the answer. Try to recall what will be displayed before clicking the empty cell.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
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Created by:
impossiblesum
Popular Histology sets