Histology ASCP Fixation information
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
|
|
||||
|---|---|---|---|---|---|
| What is the definition of a fixative? | show 🗑
|
||||
| Fixative as a process of denaturation | show 🗑
|
||||
| show | A process in which denaturation causes the protein molecule to unfold and the internal bonds to become disrupted. This disruption enables the protein to combine chemically with a fixative molecule, and the protein then becomes insoluble.
🗑
|
||||
| show | A process in which denaturation causes the protein to become less capable of maintaining a relationship with water and becomes more reactive, but the fixative molecule does not combine with the protein.
🗑
|
||||
| show | 1) Kills tissue to prevent putrefaction and autolysis
2) Maintain the proper relationship between cells and extracellular substances
3) Bring out differences in refractive indexes and to increase the visibility of different tissue elements
🗑
|
||||
| Actions of fixatives | show 🗑
|
||||
| show | A process in which the protein molecule unfolds and the internal bonds become disrupted.
🗑
|
||||
| Additive fixatives | show 🗑
|
||||
| show | Act on tissue without chemically combining with the tissue to stabilize the proteins.
🗑
|
||||
| show | Establishes a network in tissue that allows solutions to readily penetrate or gain entry into the interior of the tissue.
🗑
|
||||
| show | Creates a gel that makes penetration by subsequent solutions difficult.
🗑
|
||||
| List the coagulant fixatives | show 🗑
|
||||
| show | Formaldehyde, gluataraldehyde, glyoxal, osmium tetroxide and potassium dichromate.
🗑
|
||||
| What are factors that affect fixation? | show 🗑
|
||||
| List aqueous fixatives | show 🗑
|
||||
| Best fixatives for nucleus | show 🗑
|
||||
| show | Coalescence of chromatin into strands with intervening clear spaces.
🗑
|
||||
| show | Determined by the arrangement of covalent bonds in the amino acid sequence.
🗑
|
||||
| show | Determined by hydrogen bonding between various components of the peptide chain
🗑
|
||||
| Tertiary structure | show 🗑
|
||||
| show | Osmium tetroxide and chromic acid
🗑
|
||||
| List compound fixatives | show 🗑
|
||||
| Does not fix carbohydrates | show 🗑
|
||||
| show | Acetic Acid, Picric Acid, Ethanol
🗑
|
||||
| Penetrates rapidly | show 🗑
|
||||
| show | Acetic Acid
🗑
|
||||
| show | Acetic Acid, Formalin Ammonium Bromide, Glyoxal, Bouin, Zenker
🗑
|
||||
| show | Acetic Acid
🗑
|
||||
| Used in electron microscopy | show 🗑
|
||||
| show | Formaldehyde
🗑
|
||||
| show | Formaldehyde, Glutaraldehyde
🗑
|
||||
| Hardens tissue | show 🗑
|
||||
| show | Formaldehyde, Glyoxal, B5, Hollande
🗑
|
||||
| show | 1) Black acid hematin, birefringent formalin pigment
2) Mercury pigment, birefrigent
3) Chromic pigment
🗑
|
||||
| show | Formaldehyde
🗑
|
||||
| Hypotonic | show 🗑
|
||||
| show | 10% Formalin Saline, Modified Millonig Formalin
🗑
|
||||
| Recommended fixative for phosphlipid | show 🗑
|
||||
| show | Formalin Ammonium Bromide
🗑
|
||||
| Causes false positive Schiff Reaction | show 🗑
|
||||
| Fixes as well as dehydrates | show 🗑
|
||||
| Exposure limit of .75 ppm in 8 hour period | show 🗑
|
||||
| Exposure limit of 2 ppm over 15 minute period | show 🗑
|
||||
| Cross-links proteins | show 🗑
|
||||
| show | Glutaraldehyde, Mercuric Chloride,
🗑
|
||||
| Staining of H. Pylori unsatisfactory | show 🗑
|
||||
| Produces shrinkage | show 🗑
|
||||
| show | Osmium Tetroxide
🗑
|
||||
| Both a fixative and dye | show 🗑
|
||||
| show | Picric Acid,Ethanol, Methanol, Acetone, Gendre, Carnoy
🗑
|
||||
| show | Picric Acid, Bouin, Hollande, Zenker
🗑
|
||||
| Used for hematopoietic and lymphoreticular tissue | show 🗑
|
||||
| Leaches iron | show 🗑
|
||||
| Preserves tissue antigenicity | show 🗑
|
||||
| show | Bouin
🗑
|
||||
| Recommended for GI | show 🗑
|
||||
| show | Zenker, Helly
🗑
|
||||
| Preserves enzymes | show 🗑
|
||||
| show | Acetone
🗑
|
||||
| Fixative for touch prep and blood smears | show 🗑
|
||||
| Fixative for urate crystals | show 🗑
|
||||
| Recommended fixative over Carnoy | show 🗑
|
||||
| Mercuric Chloride, Anhydrous Sodium Acetate, Distilled Water, Formaldehyde | show 🗑
|
||||
| show | Bouin
🗑
|
||||
| show | Gendre
🗑
|
||||
| show | Hollande
🗑
|
||||
| show | Zenker
🗑
|
||||
| Mercuric Chloride, Potassium Dichromate, Sodium Sulfate, Distilled Water, Formaldehyde | show 🗑
|
||||
| show | Orth
🗑
|
||||
| Paraformaldehyde, Picric Acid, Sodium Hydroxide, Phosphate Buffer, Distilled Water | show 🗑
|
||||
| show | Carnoy
🗑
|
||||
| Absolute Methyl Alcohol, Chloroform, Glacial Acetic Acid | show 🗑
|
||||
| show | Acetic Acid
🗑
|
||||
| show | Acetic Acid,Glyoxal, Glutaraldehyde, Mercuric Chloride, Potassium Dichromate, Osmium Tetroxide, Picric Acid
🗑
|
||||
| Oxidizes carbohydrates to aldehydes | show 🗑
|
||||
| Penetrates slowly | show 🗑
|
||||
| show | Acetic Acid
🗑
|
||||
| Mordant for trichrome | show 🗑
|
||||
| Preserves chromatin and membranes | show 🗑
|
||||
| Avoid Bouin | show 🗑
|
||||
| show | Methyl Green-Pyronin Y
🗑
|
||||
| Helly satisfactory | show 🗑
|
||||
| show | Methyl Green-Pyronin Y, Congo Red
🗑
|
||||
| show | May-Grunwald Giemsa, Alcian Blue-PAS-Hematoxylin, Verhoeff, Mallory PTAH, Hotchikiss McManus PAS
🗑
|
||||
| B5 is preferred | show 🗑
|
||||
| 10% NBF satisfactory | show 🗑
|
||||
| show | PAS, Alcian Blue, Masson Trichrome, Hotchkiss-McManus PAS
🗑
|
||||
| show | Blood smears for PAS
🗑
|
||||
| Alcoholic Formalin is preferred | show 🗑
|
||||
| show | PAS D, Best Carmine, Congo Red, Crystal Violet, Turnbull Blue, Von Kossa
🗑
|
||||
| show | Best Carmine
🗑
|
||||
| Bouin is satisfactory | show 🗑
|
||||
| show | Muller Mowry Colloidal Iron, Congo Red
🗑
|
||||
| show | Muller Mowry Colloidal Iron, Aldehyde Fuchsin, Periodic Acid-Methenamine Silver, Steiner and Steiner
🗑
|
||||
| Avoid Zenker | show 🗑
|
||||
| show | Gomori 1-Step Trichrome, Van Gieson Picric Acid-Acid Fuchsin, Verhoeff, Ziehl-Neelsen
🗑
|
||||
| show | Periodic Acid-Methenamine Silver, Steiner and Steiner
🗑
|
||||
| Calcium Formalin preferred | show 🗑
|
||||
| show | Oil Red O, Sudan Black B, Fontana Masson
🗑
|
||||
| Avoid Carnoy | show 🗑
|
||||
| Avoid Methacarn | show 🗑
|
||||
| show | Oil Red O, Sudan Black B, Fontana Masson
🗑
|
||||
| show | Gomori Methenamine-Silver
🗑
|
||||
| show | Grocott Methenamine Silver
🗑
|
Review the information in the table. When you are ready to quiz yourself you can hide individual columns or the entire table. Then you can click on the empty cells to reveal the answer. Try to recall what will be displayed before clicking the empty cell.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
Created by:
impossiblesum
Popular Histology sets