WVSOM -- DNA Replication
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
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show | DNA Synthesis
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Transcription | show 🗑
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Translation | show 🗑
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show | S phase
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show | DNA Polymerase
Mg +2
Template
dNTP
Primer
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show | 5' to 3'
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Complimentary characteristic of DNA replication | show 🗑
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DNA Polymerase | show 🗑
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DNA Replication Proofreading | show 🗑
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show | it is a cofactor required for replication
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show | New DNA strand
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Primer | show 🗑
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Bidirectional | show 🗑
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show | lagging strand replicated in short stretches
Okazaki fragments
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show | 3' to 5' exonuclease activity that double checks for mistakes
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show | Deoxyribonucleotides
Building blocks of DNA
All four are required for replication
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show | Every A is paired with a T and every G with a C
Matching, mirror image sequences are called complimentary sequences
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How many charges do A and T have? | show 🗑
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show | Three
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Semiconservative | show 🗑
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show | Initiation
Extension
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What is the ori? | show 🗑
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What is a replicon? | show 🗑
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Where does replication begin? | show 🗑
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How does replication move from the replicon? | show 🗑
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What is a helicase | show 🗑
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show | SSB
Factors that stabilize single stranded DNA by preventing it from winding back into a double helix.
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What is a DNA Replication Apparatus? | show 🗑
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What are the most important factors of DNA replication apparatus? | show 🗑
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Beta clamp | show 🗑
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Leading Strand Replication | show 🗑
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show | enzyme that replicates the leading strand.
It reads the template one base at a time incorporating complementary nucleotides, ligating their 5' phosphate to the 3' OH of the growing leading strand
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Pol | show 🗑
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Discontinuous Replication of the Lagging Strand | show 🗑
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Primase | show 🗑
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show | Pol alpha
This enzyme uses the RNA primer to synthesize an okazaki fragment
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How many Okazaki fragments does Pol alpha synthesize? | show 🗑
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How long does Pol alpha replicate the okazaki fragment? | show 🗑
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show | Primase makes the next primer at th eend of the new single stranded region and the process is repeated
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What is a consequence of unwinding by helicase? | show 🗑
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show | restores the DNA to the proper level of supercoiling
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show | RNAse
DNA polymerase beta
DNA Ligase
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show | This enzyme digests any RNA
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show | This polymerase fills in gaps in DNA.
It fills in the gaps left after the RNA primers are removed.
Leaves nicks in teh DNA
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show | Binds any free 3' hydroxyls and 5' phosphates of DNA.
It seals the nicks between the okazaki fragments left by the Pol beta.
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show | Reverse transcriptase
Uses RNA as a template to make DNA
Fills in the gap from overhang that can't be filled in by the DNA polymerase.
It also extends the length of the DNA
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Created by:
tjamrose
Popular Genetics sets