Cramming some details in for clinicals during Micro rotation BH.
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| The bacterial cell wall is made from __. | peptidoglycan
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| What enzyme catalyzes formation of the linkages in the cell wall? | transpeptidase
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| What antibiotic binds to and inhibits transpeptidase? | penicillin
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| Another name for transpeptidase is __ __ __. | penicillin binding protein
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| What are the 2 layers of a gram-pos bacterial cell? | 1) Inner cytoplasmic membrane 2) Outer THICK peptidoglycan layer (60-100% made from peptidoglycan)
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| In gram-pos organisms, the lipid content is __. | low
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| Do gram-pos organisms usually have endotoxins? | No
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| Which gram-pos organism does have an endotoxin? | Listeria monocytogenese
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| Gram-pos organisms have __ periplasmic space. | no
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| So gram-pos organisms have a porin channel? | no
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| Gram-pos organisms are vulnerable to __ and __ attack. | lysozyme and penicillin
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| What are the 3 layers of a gram-neg bacterial cell? | 1) Inner cytoplasmic membrane 2) Middle THIN peptidoglycan layer 3) Outer layer with LPS (lipopolysaccharide)
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| Gram-neg organisms have a __ lipid content. | high
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| Do gram-neg organisms have endotoxins? | Yes: LPS lipid-A
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| Do gram-neg organisms have a periplasmic space? | Yes
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| Do gram-neg organisms have a porin channel? | Yes
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| Are gram-neg organisms vulnerable to penicillin or lysozyme attack? | They are resistant.
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| Rule of thumb: Gram-pos organisms are less than gram-neg or negative most of the time, except ? | Thickness of peptidoglycan layer and presence of techoic acid.
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| Gram-neg organisms have a periplasmic space filled with a gel that has __ and __. | protein and enzymes
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| Gram-neg organisms lack techoic acid but do have __ __. | murein lipoprotein
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| The outer carbohydrate chains of oligosaccharide units can serve as an __ __ in the lab. | antigenic determinant
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| What media can be used to ID Shigella and what color will it be on that media? | HE, turns metallic green
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| What media can be used to ID Salmonella and what color will it be on that media? | HE, turns black
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| MAC grows __ __ __. | gram-neg rods
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| If a colony turns __ on MAC, it is positive for lactose. | pink
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| What media is used to grow E. coli 0157? | SMAC
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| What color will E. coli turn on SMAC if it's 0157? | Pink
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| If a colony turns pink on MAC, it is positive for __. | lactose
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| What media is used to grow Campylobacter? | CAMP (or CAMPY)
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| How long to you incubate Campylobacter? | 48 hrs
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| At what temp do you incubate Campylobacter? | 42C
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| Once Campylobacter grows on CAMPY, you test for __. | oxidase
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| After you've grown Campylobacter on CAMPY and found it to be oxidase positive, how do you know if you really have Campylobacter? | A negative gram-stain is presumptive ID for Campylobacter.
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| What media is used to grow Haemophilus? | CHOC
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| What does CHOC have the BAP and others don't have that allow Haemophilus to grow well? | X and V factors
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| What do you use for urine culture? | Split TSA/MAC
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| NAP1 is a more aggressive form of __. | Campylobacter
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| Thioglycollate broth (THIO) allows for the growth of __ __. | obligate anaerobes
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| Specifically, some versions of THIO are used as a selective growth enrichment broth for __ species from contaminated sources. | Campylobacter
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| When using THIO, strict aerobes will grow only in the __ band, while microaerophiles will grow near the __ of the band where the concentration of oxygen is lower. | pink, bottom
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| Phenylethyl alcohol agar (PEA) is a selective medium used to cultivate Gram __ organisms. | gram-positive; especially Staph and Strep
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| PEA also prevents __ species from swarming across the surface of the agar. | Proteus
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| Streptococcus species can be a contaminant in specimens taken from what areas of the body? | nasopharynx, skin, vagina
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| Staphylococcus species can be a contaminant in specimens taken from what areas of the body? | skin, nares
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| Haemophilus species can be a contaminant in specimens taken from what areas of the body? | nasopharynx, upper respiratory
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| Neisseria species can be a contaminant in samples taken from what areas of the body? | nasopharynx
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| What species can be contaminants in specimens taken from the upper bowel? | Enterobactericeae, Enterococci, Candida
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| What species can be contaminants in specimens taken from the lower bowel? | Bacteroides, Clostridium
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| What species can be contaminants in specimens taken from the nasopharynx area of the body? | Streptococcus, Haemophilus, Neisseria, Candida, Actinomyces, other aerobes
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| What species can be contaminants in samples taken from the skin? | Staphylococcus, Streptococcus, Corynebacterium, Pseudomonas aeruginosa, yeasts
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| What species can be contaminants in samples taken from the vagina? | Lactobacilli, Streptococci, Corynebacteria, Candida, Actinomyces, Mycoplasma hominis
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Created by:
IsaacJ
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