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ClinMicro Cram 1

Cramming some details in for clinicals during Micro rotation BH.

QuestionAnswer
The bacterial cell wall is made from __. peptidoglycan
What enzyme catalyzes formation of the linkages in the cell wall? transpeptidase
What antibiotic binds to and inhibits transpeptidase? penicillin
Another name for transpeptidase is __ __ __. penicillin binding protein
What are the 2 layers of a gram-pos bacterial cell? 1) Inner cytoplasmic membrane 2) Outer THICK peptidoglycan layer (60-100% made from peptidoglycan)
In gram-pos organisms, the lipid content is __. low
Do gram-pos organisms usually have endotoxins? No
Which gram-pos organism does have an endotoxin? Listeria monocytogenese
Gram-pos organisms have __ periplasmic space. no
So gram-pos organisms have a porin channel? no
Gram-pos organisms are vulnerable to __ and __ attack. lysozyme and penicillin
What are the 3 layers of a gram-neg bacterial cell? 1) Inner cytoplasmic membrane 2) Middle THIN peptidoglycan layer 3) Outer layer with LPS (lipopolysaccharide)
Gram-neg organisms have a __ lipid content. high
Do gram-neg organisms have endotoxins? Yes: LPS lipid-A
Do gram-neg organisms have a periplasmic space? Yes
Do gram-neg organisms have a porin channel? Yes
Are gram-neg organisms vulnerable to penicillin or lysozyme attack? They are resistant.
Rule of thumb: Gram-pos organisms are less than gram-neg or negative most of the time, except ? Thickness of peptidoglycan layer and presence of techoic acid.
Gram-neg organisms have a periplasmic space filled with a gel that has __ and __. protein and enzymes
Gram-neg organisms lack techoic acid but do have __ __. murein lipoprotein
The outer carbohydrate chains of oligosaccharide units can serve as an __ __ in the lab. antigenic determinant
What media can be used to ID Shigella and what color will it be on that media? HE, turns metallic green
What media can be used to ID Salmonella and what color will it be on that media? HE, turns black
MAC grows __ __ __. gram-neg rods
If a colony turns __ on MAC, it is positive for lactose. pink
What media is used to grow E. coli 0157? SMAC
What color will E. coli turn on SMAC if it's 0157? Pink
If a colony turns pink on MAC, it is positive for __. lactose
What media is used to grow Campylobacter? CAMP (or CAMPY)
How long to you incubate Campylobacter? 48 hrs
At what temp do you incubate Campylobacter? 42C
Once Campylobacter grows on CAMPY, you test for __. oxidase
After you've grown Campylobacter on CAMPY and found it to be oxidase positive, how do you know if you really have Campylobacter? A negative gram-stain is presumptive ID for Campylobacter.
What media is used to grow Haemophilus? CHOC
What does CHOC have the BAP and others don't have that allow Haemophilus to grow well? X and V factors
What do you use for urine culture? Split TSA/MAC
NAP1 is a more aggressive form of __. Campylobacter
Thioglycollate broth (THIO) allows for the growth of __ __. obligate anaerobes
Specifically, some versions of THIO are used as a selective growth enrichment broth for __ species from contaminated sources. Campylobacter
When using THIO, strict aerobes will grow only in the __ band, while microaerophiles will grow near the __ of the band where the concentration of oxygen is lower. pink, bottom
Phenylethyl alcohol agar (PEA) is a selective medium used to cultivate Gram __ organisms. gram-positive; especially Staph and Strep
PEA also prevents __ species from swarming across the surface of the agar. Proteus
Streptococcus species can be a contaminant in specimens taken from what areas of the body? nasopharynx, skin, vagina
Staphylococcus species can be a contaminant in specimens taken from what areas of the body? skin, nares
Haemophilus species can be a contaminant in specimens taken from what areas of the body? nasopharynx, upper respiratory
Neisseria species can be a contaminant in samples taken from what areas of the body? nasopharynx
What species can be contaminants in specimens taken from the upper bowel? Enterobactericeae, Enterococci, Candida
What species can be contaminants in specimens taken from the lower bowel? Bacteroides, Clostridium
What species can be contaminants in specimens taken from the nasopharynx area of the body? Streptococcus, Haemophilus, Neisseria, Candida, Actinomyces, other aerobes
What species can be contaminants in samples taken from the skin? Staphylococcus, Streptococcus, Corynebacterium, Pseudomonas aeruginosa, yeasts
What species can be contaminants in samples taken from the vagina? Lactobacilli, Streptococci, Corynebacteria, Candida, Actinomyces, Mycoplasma hominis
Created by: IsaacJ
 

 



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