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Mycology - CLLS-312

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What is Mycology?   Study of fungus  
What are fungi?   microorganisms that include molds, yeast, and mushrooms  
Mycoses   fungal diseases  
How are mycoses classified   by the tissue or body site infected  
Superficial   outermost layers of skin  
cutaneous   keratin in skin, hair, and nails. causes inflammation in the skin  
subcutaneous   soft tissue, muscles, and bones immediately below the skin  
systemic   involve deep tissue and organs of the body  
Transmission of fungi   often inhalation of spores or through a puncture wound  
fungi are   eukaryotic  
Pigment may appear in what 3 places   top of colony (surface pigment), underside of colony(reverse pigment), diffused into media (diffusible pigment)  
Rugose   have furrows radiating from the center to the edge of the colony  
umbonate   raised center but may also exhibit furrows around the central button  
verucose   wrinkled surface  
textures   wooly/cottony, granular/powdery, velvety, glabrous  
wooly/cottony   dense, high aerial mycelium  
granular/powdery   very flat mycelia but produce numbers of conidia  
velvety   low, very dense aerial mycelia  
glabrous   no aerial mycelium, fungi, and yeasts will have a waxy appearance  
Sexual spores   Ascospores, Zygospores, basidiospores  
asexual spores   Sporangiospore, and Conidiospore  
aseptate   without cross walls  
septate   with cross walls  
mycelium   mass of hyphae  
conidia   asexual spores  
conidiophore   support structure for conidia  
Superficial mucoses   infections limited to the outermost layers of the skin and hair  
cutaneous mycoses   infections that extend deeper into the epidermis, as well as invasive hair and nail diseases  
dermatophytes   homogenous group of fungi that cause a variety of infections of hair, skin & nails  
Tinea capitis   ringworm of scalp  
Tinea corporis   ringworm of trunk, arms, and legs  
Tinea cruris   Jock itch-ringworm of groin  
Tinea pedis   athletes foot. ringworm of foot  
Tinea ungulum   infection of nails  
Ectothrix   infection of hair shaft surface  
endothrix   infection of hair shaft interior  
Urease   T.mentagrophytes:(+)within 7 days T.rubrum: (-)  
in vitro hair penetration   T. mentafrophytes (+) T.rubrum (-)  
growth in polished rice grains   M.audouinii: no growth other microsporum species: growth  
growth on trichophyton agars   T.violaceium, T.verrucosum, T.tonsurans: enhanced with thiamine (agar#4)  
tinea   ringworm  
3 genre of dermatophytes   Microsporum, Tichophyton, and Epidermophyton  
subcutaneous mycoses   infections involving the dermis, subcutaneous tissues, muscle & fascia  
Initiated by trauma to the skin and difficult to treat   subcutaneous mycoses  
systemic mycoses   originate primarily in lungs and may spread to other organ systems  
What is the best specimen   direct smear: some fungi can only be distinguished by micro appearance  
Methods of direct exam methods   KOH, Calcofluor White Stain, India Ink Prep, Alcian Blue/Mucicarmine stains  
Histology stains   PAS, Methenamine Silver Stain, H&E, Modified Acid Fast, Giemsa, Papanicolaou stain.  
Molds grow best in   ambient air (room temp)  
Nutritionally rich media   asexual spores  
poor nutrition media   sexual spores  


   






 
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