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Mycology - CLLS-312
| Question | Answer |
|---|---|
| What is Mycology? | Study of fungus |
| What are fungi? | microorganisms that include molds, yeast, and mushrooms |
| Mycoses | fungal diseases |
| How are mycoses classified | by the tissue or body site infected |
| Superficial | outermost layers of skin |
| cutaneous | keratin in skin, hair, and nails. causes inflammation in the skin |
| subcutaneous | soft tissue, muscles, and bones immediately below the skin |
| systemic | involve deep tissue and organs of the body |
| Transmission of fungi | often inhalation of spores or through a puncture wound |
| fungi are | eukaryotic |
| Pigment may appear in what 3 places | top of colony (surface pigment), underside of colony(reverse pigment), diffused into media (diffusible pigment) |
| Rugose | have furrows radiating from the center to the edge of the colony |
| umbonate | raised center but may also exhibit furrows around the central button |
| verucose | wrinkled surface |
| textures | wooly/cottony, granular/powdery, velvety, glabrous |
| wooly/cottony | dense, high aerial mycelium |
| granular/powdery | very flat mycelia but produce numbers of conidia |
| velvety | low, very dense aerial mycelia |
| glabrous | no aerial mycelium, fungi, and yeasts will have a waxy appearance |
| Sexual spores | Ascospores, Zygospores, basidiospores |
| asexual spores | Sporangiospore, and Conidiospore |
| aseptate | without cross walls |
| septate | with cross walls |
| mycelium | mass of hyphae |
| conidia | asexual spores |
| conidiophore | support structure for conidia |
| Superficial mucoses | infections limited to the outermost layers of the skin and hair |
| cutaneous mycoses | infections that extend deeper into the epidermis, as well as invasive hair and nail diseases |
| dermatophytes | homogenous group of fungi that cause a variety of infections of hair, skin & nails |
| Tinea capitis | ringworm of scalp |
| Tinea corporis | ringworm of trunk, arms, and legs |
| Tinea cruris | Jock itch-ringworm of groin |
| Tinea pedis | athletes foot. ringworm of foot |
| Tinea ungulum | infection of nails |
| Ectothrix | infection of hair shaft surface |
| endothrix | infection of hair shaft interior |
| Urease | T.mentagrophytes:(+)within 7 days T.rubrum: (-) |
| in vitro hair penetration | T. mentafrophytes (+) T.rubrum (-) |
| growth in polished rice grains | M.audouinii: no growth other microsporum species: growth |
| growth on trichophyton agars | T.violaceium, T.verrucosum, T.tonsurans: enhanced with thiamine (agar#4) |
| tinea | ringworm |
| 3 genre of dermatophytes | Microsporum, Tichophyton, and Epidermophyton |
| subcutaneous mycoses | infections involving the dermis, subcutaneous tissues, muscle & fascia |
| Initiated by trauma to the skin and difficult to treat | subcutaneous mycoses |
| systemic mycoses | originate primarily in lungs and may spread to other organ systems |
| What is the best specimen | direct smear: some fungi can only be distinguished by micro appearance |
| Methods of direct exam methods | KOH, Calcofluor White Stain, India Ink Prep, Alcian Blue/Mucicarmine stains |
| Histology stains | PAS, Methenamine Silver Stain, H&E, Modified Acid Fast, Giemsa, Papanicolaou stain. |
| Molds grow best in | ambient air (room temp) |
| Nutritionally rich media | asexual spores |
| poor nutrition media | sexual spores |