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Mycology - CLLS-312

What is Mycology? Study of fungus
What are fungi? microorganisms that include molds, yeast, and mushrooms
Mycoses fungal diseases
How are mycoses classified by the tissue or body site infected
Superficial outermost layers of skin
cutaneous keratin in skin, hair, and nails. causes inflammation in the skin
subcutaneous soft tissue, muscles, and bones immediately below the skin
systemic involve deep tissue and organs of the body
Transmission of fungi often inhalation of spores or through a puncture wound
fungi are eukaryotic
Pigment may appear in what 3 places top of colony (surface pigment), underside of colony(reverse pigment), diffused into media (diffusible pigment)
Rugose have furrows radiating from the center to the edge of the colony
umbonate raised center but may also exhibit furrows around the central button
verucose wrinkled surface
textures wooly/cottony, granular/powdery, velvety, glabrous
wooly/cottony dense, high aerial mycelium
granular/powdery very flat mycelia but produce numbers of conidia
velvety low, very dense aerial mycelia
glabrous no aerial mycelium, fungi, and yeasts will have a waxy appearance
Sexual spores Ascospores, Zygospores, basidiospores
asexual spores Sporangiospore, and Conidiospore
aseptate without cross walls
septate with cross walls
mycelium mass of hyphae
conidia asexual spores
conidiophore support structure for conidia
Superficial mucoses infections limited to the outermost layers of the skin and hair
cutaneous mycoses infections that extend deeper into the epidermis, as well as invasive hair and nail diseases
dermatophytes homogenous group of fungi that cause a variety of infections of hair, skin & nails
Tinea capitis ringworm of scalp
Tinea corporis ringworm of trunk, arms, and legs
Tinea cruris Jock itch-ringworm of groin
Tinea pedis athletes foot. ringworm of foot
Tinea ungulum infection of nails
Ectothrix infection of hair shaft surface
endothrix infection of hair shaft interior
Urease T.mentagrophytes:(+)within 7 days T.rubrum: (-)
in vitro hair penetration T. mentafrophytes (+) T.rubrum (-)
growth in polished rice grains M.audouinii: no growth other microsporum species: growth
growth on trichophyton agars T.violaceium, T.verrucosum, T.tonsurans: enhanced with thiamine (agar#4)
tinea ringworm
3 genre of dermatophytes Microsporum, Tichophyton, and Epidermophyton
subcutaneous mycoses infections involving the dermis, subcutaneous tissues, muscle & fascia
Initiated by trauma to the skin and difficult to treat subcutaneous mycoses
systemic mycoses originate primarily in lungs and may spread to other organ systems
What is the best specimen direct smear: some fungi can only be distinguished by micro appearance
Methods of direct exam methods KOH, Calcofluor White Stain, India Ink Prep, Alcian Blue/Mucicarmine stains
Histology stains PAS, Methenamine Silver Stain, H&E, Modified Acid Fast, Giemsa, Papanicolaou stain.
Molds grow best in ambient air (room temp)
Nutritionally rich media asexual spores
poor nutrition media sexual spores
Created by: pamela18