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Medical Laboratory Science Study Guide Chem Definitions

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Term
Definition
Electromagnetic Radiation   Has wave-like and particle-like properties.  
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Radiant Energy   Characterized as a spectrum from short wavelengths to long wavelengths  
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Wave Length   The distance traveled by one complete wave cycle measured in nanometers.  
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Nephelometry   The measurement of light scattered by a particulate solution.  
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Turbidometry   Measures the light blocked as a decrease in the light transmitted through the solution.  
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Fluorescence   A process where atoms absorb energy at a particular wavelength, and the electrons release energy as they return to ground state by emitting light energy of a longer wavelength and lower energy than the exciting wavelength.  
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Phosphorescence   The emission of light produced by certain substances after they absorb energy.  
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Chemiluminescence   The process where the chemical energy of a reaction produces excited atoms, and upon electron return to ground state, photons of light are emitted.  
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Bioluminescence   The process where an enzyme-catalyzed chemical reaction produces light emission.  
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Luminometer   A generic term for the type instrument that is used to measure chemiluminescence and bioluminescence  
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Chromatography   A technique where solutes in a sample are separated based on physical differences that allow their differential distribution between a mobile and stationary phase.  
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Mass Spectrometer   An instrument that uses the principle of charged particles moving through a magnetic or electric field, with ions being separated from other charged particles according to their mass to charge ratio.  
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Potentiometry   Technique used to determine the concentration of a substance in solution employing an electrochemical cell that consists of two half-cells, where the potential difference between an indicator electrode and a reference electrode is measured.  
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