Blood bank Exam 3
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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What is the principle of the gel test? | The controlled centrifugation of red cells through a dextran-acrylamide gel
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What are the beads in the wells do? | Serve as filters for cells----agglutinated cells get caught and non-agglutinated cells go to the bottom of the well
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Tests that are performed by gel (5) | 1. antibody screen & ID 2. ABO & Rh 3.compatibility 4. Reverse serum grouping 5. Antigen typing
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Solid phase testing | Antigen or antibody is fixed on solid matrix and specimen incubated w/coated solid phase. Corresponding Ag/Ab adheres to solid phase
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Solid phase phenotyping | antibody coated onto micro titer plate and patients RBCs added
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Solid phase phenotype--if antigen not present on RBC...... | settles to the bottom of the well
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Solid phase phenotype--if antigen present on RBC... | adheres to sides of the well
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Solid phase--Antibody ID | RBC frags on walls of micro titer well--add pt serum, incubate, wash, and RBCs coated w/IgG----Neg=cells on bottom Pos=cells on sides of well
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