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Gel & solid phase

Blood bank Exam 3

What is the principle of the gel test? The controlled centrifugation of red cells through a dextran-acrylamide gel
What are the beads in the wells do? Serve as filters for cells----agglutinated cells get caught and non-agglutinated cells go to the bottom of the well
Tests that are performed by gel (5) 1. antibody screen & ID 2. ABO & Rh 3.compatibility 4. Reverse serum grouping 5. Antigen typing
Solid phase testing Antigen or antibody is fixed on solid matrix and specimen incubated w/coated solid phase. Corresponding Ag/Ab adheres to solid phase
Solid phase phenotyping antibody coated onto micro titer plate and patients RBCs added
Solid phase phenotype--if antigen not present on RBC...... settles to the bottom of the well
Solid phase phenotype--if antigen present on RBC... adheres to sides of the well
Solid phase--Antibody ID RBC frags on walls of micro titer well--add pt serum, incubate, wash, and RBCs coated w/IgG----Neg=cells on bottom Pos=cells on sides of well
Created by: aenewsom