Chp30 in Hem2 for DelTech Owens classes
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| Cytochemical stains aid in differentiating morphologically similar cells by identifying what 4 things? | 1) enzymes 2) lipids 3) glycogen 4) other substances in cells
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| Cytochemical reactions can be __ or __. | enzymatic or nonenzymatic
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| __ smears must be used to detect enzymatic activity. | fresh
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| Nonenzymatic procedures may be performed on specimens that have been stored at __ __. | room temperature
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| MPO stains __ granules. | primary
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| MPO is useful in differentiating __ from __ cells. | granulocytic from lymphoid
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| SBB stains __ and has similar results to MPO stain. | lipids
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| Cells of monocytic origin can be differentiated using __. | esterases
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| PAS stains yield positive results in specimens with __. | ALLs
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| PAS stains yield positive results in specimens with __ __ in erythroid __ and __ syndromes. | erythroid precursors and myelodysplastic syndromes
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| LAP is most useful in distinguishing __ from __ reactions. | CML from leukomoid reactions
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| Specimens from patients with CML have very low __ scores. | LAP
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| Hairy cell leukemia specimens yield positive results if stained with __ phosphatase with __ inhibition. | acid phosphatase with tartrate inhibition
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| For most cytochemical stains, a blood film containing what 3 kinds of cells is acceptable as a control sample? | neutrophils, lymphocytes, monocytes
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| SBB smears may be stable for __ if stored at room temperature. | months
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| Which cytochemical stain can be used to differentiate AML from ALL? | MPO
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| SBB stains the __ components of cells. | lipid
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| Hairy cell leukemia cells produce an abundance of isoenzyme _. | isoenzyme 5
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| An LAP score of 250 is consistent with a diagnosis of __ reaction. | leukemoid reaction
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| a-naphthyl butyrate is __ esterase. | nonspecific
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| a-naphthyl butryate reacts with cells of which WBC lineage? | monocytic
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| LAP is used to evaluate mature __ and __. | neutrophils and bands
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| What 3 stains will yield a positive result for a normal blood film? | 1) MPO 2) a-Naphthyl acetate esterase 3) LAP
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| True or false: Phsphate buffered saline is a suitable fixative for slides that are to be cytochemically stained? | false
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| Methanol, acetone, and formaldehyde are suitable for slides that are to be __ stained. | cytochemically
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Created by:
IsaacJ
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