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Hem2 Chp30 practice
Chp30 in Hem2 for DelTech Owens classes
Question | Answer |
---|---|
Cytochemical stains aid in differentiating morphologically similar cells by identifying what 4 things? | 1) enzymes 2) lipids 3) glycogen 4) other substances in cells |
Cytochemical reactions can be __ or __. | enzymatic or nonenzymatic |
__ smears must be used to detect enzymatic activity. | fresh |
Nonenzymatic procedures may be performed on specimens that have been stored at __ __. | room temperature |
MPO stains __ granules. | primary |
MPO is useful in differentiating __ from __ cells. | granulocytic from lymphoid |
SBB stains __ and has similar results to MPO stain. | lipids |
Cells of monocytic origin can be differentiated using __. | esterases |
PAS stains yield positive results in specimens with __. | ALLs |
PAS stains yield positive results in specimens with __ __ in erythroid __ and __ syndromes. | erythroid precursors and myelodysplastic syndromes |
LAP is most useful in distinguishing __ from __ reactions. | CML from leukomoid reactions |
Specimens from patients with CML have very low __ scores. | LAP |
Hairy cell leukemia specimens yield positive results if stained with __ phosphatase with __ inhibition. | acid phosphatase with tartrate inhibition |
For most cytochemical stains, a blood film containing what 3 kinds of cells is acceptable as a control sample? | neutrophils, lymphocytes, monocytes |
SBB smears may be stable for __ if stored at room temperature. | months |
Which cytochemical stain can be used to differentiate AML from ALL? | MPO |
SBB stains the __ components of cells. | lipid |
Hairy cell leukemia cells produce an abundance of isoenzyme _. | isoenzyme 5 |
An LAP score of 250 is consistent with a diagnosis of __ reaction. | leukemoid reaction |
a-naphthyl butyrate is __ esterase. | nonspecific |
a-naphthyl butryate reacts with cells of which WBC lineage? | monocytic |
LAP is used to evaluate mature __ and __. | neutrophils and bands |
What 3 stains will yield a positive result for a normal blood film? | 1) MPO 2) a-Naphthyl acetate esterase 3) LAP |
True or false: Phsphate buffered saline is a suitable fixative for slides that are to be cytochemically stained? | false |
Methanol, acetone, and formaldehyde are suitable for slides that are to be __ stained. | cytochemically |