Stack #105900
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| KOH prep can be used to examine | Hair, nails, skin scrapings, fluids, exudates, biopsies
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| What does the KOH do to the specimen? | Dissolves specimen quickly (fungi slowly)
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| What happens if calcofluor white is added to KOH? | Binds to cell wall and fluoresces blue-white under UV light
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| Historical use of India Ink | CSF specimens for C. neoformans
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| What does India Ink do? | Creates black background to visualize capsul
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| Giemsa, Wright-Giemsa | H. capsulatum (intracellular)
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| Hematoxylin and Eosin (H&E) | Pink to pinkish blue
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| Meyer's Mucicarmine | C. neoformans - rose red
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| Gomori Methenamine Silver (GMS) | Fungal elements are black
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| Papanicolaou stain | Pink to blue (cervical, vaginal, some resp samples)
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| Periodic Acid Schiff (PAS) | Red or purple
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| Time need for yeast to grow | 2 to 3 days
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| Rapid growing mould grows in | Less than 5 days
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| Intermediate mould grows in | 6 to 10 days
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| Slow growing mould takes how long to grow? | More than 11 days, sometimes 8 weeks
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| 3 methods used in microscopic examination of moulds | Tease/cut preperation, Scotch tape preperation, Slide culture
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| Tease/cut preperation | Organism removed directly from plate, teased apart with teasing needles, can also cut out a piece
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| Scotch tape preperation | Scotch tape pressed onto culture plate, transferred to microscope slide
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| Slide culture | Organism is sub-cultured to small piece of agar, covered with coverslip, grows onto coverslip which is then removed and examined
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| Best microscopic method for examining moulds is | Slide culture
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| What stain is usually added for microscopic examination? | Lactophenol-cotton blue
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| Hair perforation test | Sterile hair floated on sterile water + yeast extract, conidia or hyphae inoculated onto water surface, remove hair shafts and observe weekly for 1 month
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| Trichophyton hair perforation test results | T. rubrum = Neg; T. mentagrophytes = Pos
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| Urease test | Tubes of urease agar are lightly inoculated, incubated 5 days at room temp
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| Trichophyton urease test results | T. mentagrophytes = Pos, T. rubrum = Neg or weak
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| Trichophyton agars for dermatophyte identification | T agar #1 w/o thiamine, T agar #4 w/thiamine; incubate for 10-14 days and observe for growth
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| T. mentagrophytes growth on Trichophyton agar | 4+ on #1 and #4
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| T. rubrum growth on Trichophyton agar | 4+ on #1 and #4
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| T. tonsurans growth on Trichophyton agar | +/- on #1 and 4+ on #4
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| Rice grain growth | Sterile, nonfortified rice grain media; incubate 10 days and observe for growth
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| Rice grain growth is used to differentiate | M. canis (grows) vs. M. audouinii
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