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Module 2 Lab Info
Stack #105900
| Question | Answer |
|---|---|
| KOH prep can be used to examine | Hair, nails, skin scrapings, fluids, exudates, biopsies |
| What does the KOH do to the specimen? | Dissolves specimen quickly (fungi slowly) |
| What happens if calcofluor white is added to KOH? | Binds to cell wall and fluoresces blue-white under UV light |
| Historical use of India Ink | CSF specimens for C. neoformans |
| What does India Ink do? | Creates black background to visualize capsul |
| Giemsa, Wright-Giemsa | H. capsulatum (intracellular) |
| Hematoxylin and Eosin (H&E) | Pink to pinkish blue |
| Meyer's Mucicarmine | C. neoformans - rose red |
| Gomori Methenamine Silver (GMS) | Fungal elements are black |
| Papanicolaou stain | Pink to blue (cervical, vaginal, some resp samples) |
| Periodic Acid Schiff (PAS) | Red or purple |
| Time need for yeast to grow | 2 to 3 days |
| Rapid growing mould grows in | Less than 5 days |
| Intermediate mould grows in | 6 to 10 days |
| Slow growing mould takes how long to grow? | More than 11 days, sometimes 8 weeks |
| 3 methods used in microscopic examination of moulds | Tease/cut preperation, Scotch tape preperation, Slide culture |
| Tease/cut preperation | Organism removed directly from plate, teased apart with teasing needles, can also cut out a piece |
| Scotch tape preperation | Scotch tape pressed onto culture plate, transferred to microscope slide |
| Slide culture | Organism is sub-cultured to small piece of agar, covered with coverslip, grows onto coverslip which is then removed and examined |
| Best microscopic method for examining moulds is | Slide culture |
| What stain is usually added for microscopic examination? | Lactophenol-cotton blue |
| Hair perforation test | Sterile hair floated on sterile water + yeast extract, conidia or hyphae inoculated onto water surface, remove hair shafts and observe weekly for 1 month |
| Trichophyton hair perforation test results | T. rubrum = Neg; T. mentagrophytes = Pos |
| Urease test | Tubes of urease agar are lightly inoculated, incubated 5 days at room temp |
| Trichophyton urease test results | T. mentagrophytes = Pos, T. rubrum = Neg or weak |
| Trichophyton agars for dermatophyte identification | T agar #1 w/o thiamine, T agar #4 w/thiamine; incubate for 10-14 days and observe for growth |
| T. mentagrophytes growth on Trichophyton agar | 4+ on #1 and #4 |
| T. rubrum growth on Trichophyton agar | 4+ on #1 and #4 |
| T. tonsurans growth on Trichophyton agar | +/- on #1 and 4+ on #4 |
| Rice grain growth | Sterile, nonfortified rice grain media; incubate 10 days and observe for growth |
| Rice grain growth is used to differentiate | M. canis (grows) vs. M. audouinii |
Created by:
blackicecreamcat
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